Cargando…
Genome-wide analysis of the SPL/miR156 module and its interaction with the AP2/miR172 unit in barley
The SQUAMOSA-promoter binding like (SPL) gene family encodes transcription factors that have been shown in many species to influence plant growth and development, but information about these genes in barley (Hordeum vulgare L.) is limited. This study identified 17 barley SPL genes, within eight dist...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5935748/ https://www.ncbi.nlm.nih.gov/pubmed/29728569 http://dx.doi.org/10.1038/s41598-018-25349-0 |
_version_ | 1783320322460614656 |
---|---|
author | Tripathi, Rajiv K. Bregitzer, Phil Singh, Jaswinder |
author_facet | Tripathi, Rajiv K. Bregitzer, Phil Singh, Jaswinder |
author_sort | Tripathi, Rajiv K. |
collection | PubMed |
description | The SQUAMOSA-promoter binding like (SPL) gene family encodes transcription factors that have been shown in many species to influence plant growth and development, but information about these genes in barley (Hordeum vulgare L.) is limited. This study identified 17 barley SPL genes, within eight distinct groups, that are orthologs of SPL genes described in Arabidopsis, wheat, and rice. Sixteen barley SPLs undergo alternative splicing. Seven SPLs contain a putative miR156 target site and the transcript levels of the miR156-targeted HvSPLs (HvSPL3, 13 and 23) were lower in vegetative than in reproductive phase but this was true also for some SPL genes such as HvSPL6 that were not regulated by miR156. Because SPL gene products regulate miR172, which is also involved in floral development, the expression of miR172 was studied. An antagonistic expression pattern of miR156 and miR172b during the vegetative and the reproductive phases signifies their apparent function in barley growth phase transition. Characterization of a barley mir172 mutant having an abnormal, indeterminate spikelet phenotype suggests the possible feedback role of AP2/miR172 module on HvSPL genes. This is the first comprehensive analysis of the miR156/SPL/miR172 axis in barley that provides a basis to elucidate their roles in various biological processes. |
format | Online Article Text |
id | pubmed-5935748 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59357482018-05-10 Genome-wide analysis of the SPL/miR156 module and its interaction with the AP2/miR172 unit in barley Tripathi, Rajiv K. Bregitzer, Phil Singh, Jaswinder Sci Rep Article The SQUAMOSA-promoter binding like (SPL) gene family encodes transcription factors that have been shown in many species to influence plant growth and development, but information about these genes in barley (Hordeum vulgare L.) is limited. This study identified 17 barley SPL genes, within eight distinct groups, that are orthologs of SPL genes described in Arabidopsis, wheat, and rice. Sixteen barley SPLs undergo alternative splicing. Seven SPLs contain a putative miR156 target site and the transcript levels of the miR156-targeted HvSPLs (HvSPL3, 13 and 23) were lower in vegetative than in reproductive phase but this was true also for some SPL genes such as HvSPL6 that were not regulated by miR156. Because SPL gene products regulate miR172, which is also involved in floral development, the expression of miR172 was studied. An antagonistic expression pattern of miR156 and miR172b during the vegetative and the reproductive phases signifies their apparent function in barley growth phase transition. Characterization of a barley mir172 mutant having an abnormal, indeterminate spikelet phenotype suggests the possible feedback role of AP2/miR172 module on HvSPL genes. This is the first comprehensive analysis of the miR156/SPL/miR172 axis in barley that provides a basis to elucidate their roles in various biological processes. Nature Publishing Group UK 2018-05-04 /pmc/articles/PMC5935748/ /pubmed/29728569 http://dx.doi.org/10.1038/s41598-018-25349-0 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Tripathi, Rajiv K. Bregitzer, Phil Singh, Jaswinder Genome-wide analysis of the SPL/miR156 module and its interaction with the AP2/miR172 unit in barley |
title | Genome-wide analysis of the SPL/miR156 module and its interaction with the AP2/miR172 unit in barley |
title_full | Genome-wide analysis of the SPL/miR156 module and its interaction with the AP2/miR172 unit in barley |
title_fullStr | Genome-wide analysis of the SPL/miR156 module and its interaction with the AP2/miR172 unit in barley |
title_full_unstemmed | Genome-wide analysis of the SPL/miR156 module and its interaction with the AP2/miR172 unit in barley |
title_short | Genome-wide analysis of the SPL/miR156 module and its interaction with the AP2/miR172 unit in barley |
title_sort | genome-wide analysis of the spl/mir156 module and its interaction with the ap2/mir172 unit in barley |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5935748/ https://www.ncbi.nlm.nih.gov/pubmed/29728569 http://dx.doi.org/10.1038/s41598-018-25349-0 |
work_keys_str_mv | AT tripathirajivk genomewideanalysisofthesplmir156moduleanditsinteractionwiththeap2mir172unitinbarley AT bregitzerphil genomewideanalysisofthesplmir156moduleanditsinteractionwiththeap2mir172unitinbarley AT singhjaswinder genomewideanalysisofthesplmir156moduleanditsinteractionwiththeap2mir172unitinbarley |