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Alginate nanobeads interspersed fibrin network as in situ forming hydrogel for soft tissue engineering

Hydrogels are a class of materials that has the property of injectability and in situ gel formation. This property of hydrogels is manipulated in this study to develop a biomimetic bioresorbable injectable system of alginate nanobeads interspersed in fibrin network. Alginate nanobeads developed by c...

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Detalles Bibliográficos
Autores principales: Deepthi, S., Jayakumar, R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5935774/
https://www.ncbi.nlm.nih.gov/pubmed/29744457
http://dx.doi.org/10.1016/j.bioactmat.2017.09.005
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author Deepthi, S.
Jayakumar, R.
author_facet Deepthi, S.
Jayakumar, R.
author_sort Deepthi, S.
collection PubMed
description Hydrogels are a class of materials that has the property of injectability and in situ gel formation. This property of hydrogels is manipulated in this study to develop a biomimetic bioresorbable injectable system of alginate nanobeads interspersed in fibrin network. Alginate nanobeads developed by calcium cross-linking yielded a size of 200–500 nm. The alginate nanobeads fibrin hydrogel was formed using dual syringe apparatus. Characterization of the in situ injectable hydrogel was done by SEM, FTIR and Rheometer. The developed hydrogel showed mechanical strength of 19 kPa which provides the suitable compliance for soft tissue engineering. Cytocompatibility studies using human umbilical cord blood derived mesenchymal stem cells showed good attachment, proliferation and infiltration within the hydrogel similar to fibrin gel. The developed in situ forming hydrogel could be a suitable delivery carrier of stem cells for soft tissue regeneration.
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spelling pubmed-59357742018-05-09 Alginate nanobeads interspersed fibrin network as in situ forming hydrogel for soft tissue engineering Deepthi, S. Jayakumar, R. Bioact Mater Article Hydrogels are a class of materials that has the property of injectability and in situ gel formation. This property of hydrogels is manipulated in this study to develop a biomimetic bioresorbable injectable system of alginate nanobeads interspersed in fibrin network. Alginate nanobeads developed by calcium cross-linking yielded a size of 200–500 nm. The alginate nanobeads fibrin hydrogel was formed using dual syringe apparatus. Characterization of the in situ injectable hydrogel was done by SEM, FTIR and Rheometer. The developed hydrogel showed mechanical strength of 19 kPa which provides the suitable compliance for soft tissue engineering. Cytocompatibility studies using human umbilical cord blood derived mesenchymal stem cells showed good attachment, proliferation and infiltration within the hydrogel similar to fibrin gel. The developed in situ forming hydrogel could be a suitable delivery carrier of stem cells for soft tissue regeneration. KeAi Publishing 2017-09-30 /pmc/articles/PMC5935774/ /pubmed/29744457 http://dx.doi.org/10.1016/j.bioactmat.2017.09.005 Text en © 2017 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Deepthi, S.
Jayakumar, R.
Alginate nanobeads interspersed fibrin network as in situ forming hydrogel for soft tissue engineering
title Alginate nanobeads interspersed fibrin network as in situ forming hydrogel for soft tissue engineering
title_full Alginate nanobeads interspersed fibrin network as in situ forming hydrogel for soft tissue engineering
title_fullStr Alginate nanobeads interspersed fibrin network as in situ forming hydrogel for soft tissue engineering
title_full_unstemmed Alginate nanobeads interspersed fibrin network as in situ forming hydrogel for soft tissue engineering
title_short Alginate nanobeads interspersed fibrin network as in situ forming hydrogel for soft tissue engineering
title_sort alginate nanobeads interspersed fibrin network as in situ forming hydrogel for soft tissue engineering
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5935774/
https://www.ncbi.nlm.nih.gov/pubmed/29744457
http://dx.doi.org/10.1016/j.bioactmat.2017.09.005
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