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Alginate nanobeads interspersed fibrin network as in situ forming hydrogel for soft tissue engineering
Hydrogels are a class of materials that has the property of injectability and in situ gel formation. This property of hydrogels is manipulated in this study to develop a biomimetic bioresorbable injectable system of alginate nanobeads interspersed in fibrin network. Alginate nanobeads developed by c...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5935774/ https://www.ncbi.nlm.nih.gov/pubmed/29744457 http://dx.doi.org/10.1016/j.bioactmat.2017.09.005 |
_version_ | 1783320328212054016 |
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author | Deepthi, S. Jayakumar, R. |
author_facet | Deepthi, S. Jayakumar, R. |
author_sort | Deepthi, S. |
collection | PubMed |
description | Hydrogels are a class of materials that has the property of injectability and in situ gel formation. This property of hydrogels is manipulated in this study to develop a biomimetic bioresorbable injectable system of alginate nanobeads interspersed in fibrin network. Alginate nanobeads developed by calcium cross-linking yielded a size of 200–500 nm. The alginate nanobeads fibrin hydrogel was formed using dual syringe apparatus. Characterization of the in situ injectable hydrogel was done by SEM, FTIR and Rheometer. The developed hydrogel showed mechanical strength of 19 kPa which provides the suitable compliance for soft tissue engineering. Cytocompatibility studies using human umbilical cord blood derived mesenchymal stem cells showed good attachment, proliferation and infiltration within the hydrogel similar to fibrin gel. The developed in situ forming hydrogel could be a suitable delivery carrier of stem cells for soft tissue regeneration. |
format | Online Article Text |
id | pubmed-5935774 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | KeAi Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-59357742018-05-09 Alginate nanobeads interspersed fibrin network as in situ forming hydrogel for soft tissue engineering Deepthi, S. Jayakumar, R. Bioact Mater Article Hydrogels are a class of materials that has the property of injectability and in situ gel formation. This property of hydrogels is manipulated in this study to develop a biomimetic bioresorbable injectable system of alginate nanobeads interspersed in fibrin network. Alginate nanobeads developed by calcium cross-linking yielded a size of 200–500 nm. The alginate nanobeads fibrin hydrogel was formed using dual syringe apparatus. Characterization of the in situ injectable hydrogel was done by SEM, FTIR and Rheometer. The developed hydrogel showed mechanical strength of 19 kPa which provides the suitable compliance for soft tissue engineering. Cytocompatibility studies using human umbilical cord blood derived mesenchymal stem cells showed good attachment, proliferation and infiltration within the hydrogel similar to fibrin gel. The developed in situ forming hydrogel could be a suitable delivery carrier of stem cells for soft tissue regeneration. KeAi Publishing 2017-09-30 /pmc/articles/PMC5935774/ /pubmed/29744457 http://dx.doi.org/10.1016/j.bioactmat.2017.09.005 Text en © 2017 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Deepthi, S. Jayakumar, R. Alginate nanobeads interspersed fibrin network as in situ forming hydrogel for soft tissue engineering |
title | Alginate nanobeads interspersed fibrin network as in situ forming hydrogel for soft tissue engineering |
title_full | Alginate nanobeads interspersed fibrin network as in situ forming hydrogel for soft tissue engineering |
title_fullStr | Alginate nanobeads interspersed fibrin network as in situ forming hydrogel for soft tissue engineering |
title_full_unstemmed | Alginate nanobeads interspersed fibrin network as in situ forming hydrogel for soft tissue engineering |
title_short | Alginate nanobeads interspersed fibrin network as in situ forming hydrogel for soft tissue engineering |
title_sort | alginate nanobeads interspersed fibrin network as in situ forming hydrogel for soft tissue engineering |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5935774/ https://www.ncbi.nlm.nih.gov/pubmed/29744457 http://dx.doi.org/10.1016/j.bioactmat.2017.09.005 |
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