Application of a Homogenous Assay for the Detection of 2,4,6-Trinitrotoluene to Environmental Water Samples

A homogeneous assay was used to detect 2,4,6-trinitrotoluene (TNT) spiked into environmental water samples. This assay is based on changes in fluorescence emission intensity when TNT competitively displaces a fluorescently labeled, TNT analog bound to an anti-TNT antibody. The effectiveness of the a...

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Autores principales: Goldman, Ellen R., Egge, Adrienne L., Medintz, Igor L., Lassman, Michael E., Anderson, George P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2005
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5936517/
https://www.ncbi.nlm.nih.gov/pubmed/15915298
http://dx.doi.org/10.1100/tsw.2005.58
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author Goldman, Ellen R.
Egge, Adrienne L.
Medintz, Igor L.
Lassman, Michael E.
Anderson, George P.
author_facet Goldman, Ellen R.
Egge, Adrienne L.
Medintz, Igor L.
Lassman, Michael E.
Anderson, George P.
author_sort Goldman, Ellen R.
collection PubMed
description A homogeneous assay was used to detect 2,4,6-trinitrotoluene (TNT) spiked into environmental water samples. This assay is based on changes in fluorescence emission intensity when TNT competitively displaces a fluorescently labeled, TNT analog bound to an anti-TNT antibody. The effectiveness of the assay was highly dependent on the source of the sample being tested. As no correlation between pH and assay performance was observed, ionic strength was assumed to be the reason for variation in assay results. Addition of 10x phosphate-buffered saline to samples to increase their ionic strength to that of our standard laboratory buffer (about 0.17 M) significantly improved the range over which the assay functioned in several river water samples.
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spelling pubmed-59365172018-06-03 Application of a Homogenous Assay for the Detection of 2,4,6-Trinitrotoluene to Environmental Water Samples Goldman, Ellen R. Egge, Adrienne L. Medintz, Igor L. Lassman, Michael E. Anderson, George P. ScientificWorldJournal Research Article A homogeneous assay was used to detect 2,4,6-trinitrotoluene (TNT) spiked into environmental water samples. This assay is based on changes in fluorescence emission intensity when TNT competitively displaces a fluorescently labeled, TNT analog bound to an anti-TNT antibody. The effectiveness of the assay was highly dependent on the source of the sample being tested. As no correlation between pH and assay performance was observed, ionic strength was assumed to be the reason for variation in assay results. Addition of 10x phosphate-buffered saline to samples to increase their ionic strength to that of our standard laboratory buffer (about 0.17 M) significantly improved the range over which the assay functioned in several river water samples. TheScientificWorldJOURNAL 2005-05-23 /pmc/articles/PMC5936517/ /pubmed/15915298 http://dx.doi.org/10.1100/tsw.2005.58 Text en Copyright © 2005 Ellen R. Goldman et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Goldman, Ellen R.
Egge, Adrienne L.
Medintz, Igor L.
Lassman, Michael E.
Anderson, George P.
Application of a Homogenous Assay for the Detection of 2,4,6-Trinitrotoluene to Environmental Water Samples
title Application of a Homogenous Assay for the Detection of 2,4,6-Trinitrotoluene to Environmental Water Samples
title_full Application of a Homogenous Assay for the Detection of 2,4,6-Trinitrotoluene to Environmental Water Samples
title_fullStr Application of a Homogenous Assay for the Detection of 2,4,6-Trinitrotoluene to Environmental Water Samples
title_full_unstemmed Application of a Homogenous Assay for the Detection of 2,4,6-Trinitrotoluene to Environmental Water Samples
title_short Application of a Homogenous Assay for the Detection of 2,4,6-Trinitrotoluene to Environmental Water Samples
title_sort application of a homogenous assay for the detection of 2,4,6-trinitrotoluene to environmental water samples
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5936517/
https://www.ncbi.nlm.nih.gov/pubmed/15915298
http://dx.doi.org/10.1100/tsw.2005.58
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