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Antibody Mediated Transduction of Therapeutic Proteins into Living Cells
Protein therapy refers to the direct delivery of therapeutic proteins to cells and tissues with the goal of ameliorating or modifying a disease process. Current techniques for delivering proteins across cell membranes include taking advantage of receptor-mediated endocytosis or using protein transdu...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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TheScientificWorldJOURNAL
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5936519/ https://www.ncbi.nlm.nih.gov/pubmed/16170440 http://dx.doi.org/10.1100/tsw.2005.98 |
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author | Hansen, James E. Weisbart, Richard H. Nishimura, Robert N. |
author_facet | Hansen, James E. Weisbart, Richard H. Nishimura, Robert N. |
author_sort | Hansen, James E. |
collection | PubMed |
description | Protein therapy refers to the direct delivery of therapeutic proteins to cells and tissues with the goal of ameliorating or modifying a disease process. Current techniques for delivering proteins across cell membranes include taking advantage of receptor-mediated endocytosis or using protein transduction domains that penetrate directly into cells. The most commonly used protein transduction domains are small cell-penetrating peptides derived from such proteins as the HIV-1 Tat protein. A novel protein transduction domain developed as the single chain fragment (Fv) of a murine anti-DNA autoantibody, mAb 3E10, has recently been developed and used to deliver biologically active proteins to living cells in vitro. This review will provide a brief overview of the development of the Fv fragment and provide a summary of recent studies using Fv to deliver therapeutic peptides and proteins (such as a C-terminal p53 peptide, C-terminal p53 antibody fragment, full-length p53, and micro-dystrophin) to cells. |
format | Online Article Text |
id | pubmed-5936519 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | TheScientificWorldJOURNAL |
record_format | MEDLINE/PubMed |
spelling | pubmed-59365192018-06-03 Antibody Mediated Transduction of Therapeutic Proteins into Living Cells Hansen, James E. Weisbart, Richard H. Nishimura, Robert N. ScientificWorldJournal Mini-Review Article Protein therapy refers to the direct delivery of therapeutic proteins to cells and tissues with the goal of ameliorating or modifying a disease process. Current techniques for delivering proteins across cell membranes include taking advantage of receptor-mediated endocytosis or using protein transduction domains that penetrate directly into cells. The most commonly used protein transduction domains are small cell-penetrating peptides derived from such proteins as the HIV-1 Tat protein. A novel protein transduction domain developed as the single chain fragment (Fv) of a murine anti-DNA autoantibody, mAb 3E10, has recently been developed and used to deliver biologically active proteins to living cells in vitro. This review will provide a brief overview of the development of the Fv fragment and provide a summary of recent studies using Fv to deliver therapeutic peptides and proteins (such as a C-terminal p53 peptide, C-terminal p53 antibody fragment, full-length p53, and micro-dystrophin) to cells. TheScientificWorldJOURNAL 2005-09-16 /pmc/articles/PMC5936519/ /pubmed/16170440 http://dx.doi.org/10.1100/tsw.2005.98 Text en Copyright © 2005 James E. Hansen et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Mini-Review Article Hansen, James E. Weisbart, Richard H. Nishimura, Robert N. Antibody Mediated Transduction of Therapeutic Proteins into Living Cells |
title | Antibody Mediated Transduction of Therapeutic Proteins into Living Cells |
title_full | Antibody Mediated Transduction of Therapeutic Proteins into Living Cells |
title_fullStr | Antibody Mediated Transduction of Therapeutic Proteins into Living Cells |
title_full_unstemmed | Antibody Mediated Transduction of Therapeutic Proteins into Living Cells |
title_short | Antibody Mediated Transduction of Therapeutic Proteins into Living Cells |
title_sort | antibody mediated transduction of therapeutic proteins into living cells |
topic | Mini-Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5936519/ https://www.ncbi.nlm.nih.gov/pubmed/16170440 http://dx.doi.org/10.1100/tsw.2005.98 |
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