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Nanostructured Ag(+)-substituted fluorhydroxyapatite-TiO(2) coatings for enhanced bactericidal effects and osteoinductivity of Ti for biomedical applications
BACKGROUND: Poor mechanical properties, undesirable fast dissolution rate, and lack of antibacterial activity limit the application of hydroxyapatite (HA) as an implant coating material. To overcome these limitations, a hybrid coating of Ag(+)-substituted fluorhydroxyapatite and titania nanotube (TN...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5937497/ https://www.ncbi.nlm.nih.gov/pubmed/29760549 http://dx.doi.org/10.2147/IJN.S162558 |
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author | Huang, Yong Song, Guiqin Chang, Xiaotong Wang, Zhenhui Zhang, Xuejiao Han, Shuguang Su, Zhuobin Yang, Hejie Yang, Dongdong Zhang, Xiaojun |
author_facet | Huang, Yong Song, Guiqin Chang, Xiaotong Wang, Zhenhui Zhang, Xuejiao Han, Shuguang Su, Zhuobin Yang, Hejie Yang, Dongdong Zhang, Xiaojun |
author_sort | Huang, Yong |
collection | PubMed |
description | BACKGROUND: Poor mechanical properties, undesirable fast dissolution rate, and lack of antibacterial activity limit the application of hydroxyapatite (HA) as an implant coating material. To overcome these limitations, a hybrid coating of Ag(+)-substituted fluorhydroxyapatite and titania nanotube (TNT) was prepared. METHODS: The incorporation of silver into the HA-TiO(2) hybrid coating improves its antimicrobial properties. The addition of F as a second binary element increases the structural stability of the coating. The TNT/F-and-Ag-substituted HA (FAgHA) bilayer coating on the Ti substrate was confirmed by X-ray diffraction, scanning electron microscope, energy-dispersive X-ray spectroscopy (EDS), and X-ray photoelectron spectroscopy (XPS). RESULTS: The results indicate that the FAgHA/TNT nanocomposite coating has a dense and uniform morphology with a nano-rod-like structure. The solubility measurement result shows that the substitution of F(−) ions into the AgHA structure has a positive effect on the dissolution resistance of HA. The adhesion strength of FAgHA/TNT has significantly increased because of the interlocking of the roughened surface with nano-rod-like particles that entered into the voids of the TiO(2) nanotubes. Compared with that of the bare Ti, the corrosion current density of FAgHA/TNT-coated Ti substrate decreased from 3.71 to 0.18 μA, and its corrosion resistance increased by almost two orders of magnitude. Moreover, despite pure HA, the FAgHA killed all viable Staphylococcus aureus after 24 hours of incubation. Although the fabricated FAgHA/TNT coating is hydrophobic, it induced deposition of the typical spherical apatite when immersed in a simulated body fluid (SBF); the osteoblasts spread very well on the surface of the coating. In addition, in vitro cell culture tests demonstrated cell viability and alkaline phosphatase (ALP) similar to pure HA, which indicated good cytocompatibility. Interestingly, compared with bare Ti, FAgHA/TNT-coated Ti surface was innocent for cell vitality and even more beneficial for cell osteogenesis in vitro. CONCLUSION: Enhancing the osseointegration and preventing infection in implants, the FAgHA/TNT-coated Ti makes implants more successful. |
format | Online Article Text |
id | pubmed-5937497 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-59374972018-05-14 Nanostructured Ag(+)-substituted fluorhydroxyapatite-TiO(2) coatings for enhanced bactericidal effects and osteoinductivity of Ti for biomedical applications Huang, Yong Song, Guiqin Chang, Xiaotong Wang, Zhenhui Zhang, Xuejiao Han, Shuguang Su, Zhuobin Yang, Hejie Yang, Dongdong Zhang, Xiaojun Int J Nanomedicine Original Research BACKGROUND: Poor mechanical properties, undesirable fast dissolution rate, and lack of antibacterial activity limit the application of hydroxyapatite (HA) as an implant coating material. To overcome these limitations, a hybrid coating of Ag(+)-substituted fluorhydroxyapatite and titania nanotube (TNT) was prepared. METHODS: The incorporation of silver into the HA-TiO(2) hybrid coating improves its antimicrobial properties. The addition of F as a second binary element increases the structural stability of the coating. The TNT/F-and-Ag-substituted HA (FAgHA) bilayer coating on the Ti substrate was confirmed by X-ray diffraction, scanning electron microscope, energy-dispersive X-ray spectroscopy (EDS), and X-ray photoelectron spectroscopy (XPS). RESULTS: The results indicate that the FAgHA/TNT nanocomposite coating has a dense and uniform morphology with a nano-rod-like structure. The solubility measurement result shows that the substitution of F(−) ions into the AgHA structure has a positive effect on the dissolution resistance of HA. The adhesion strength of FAgHA/TNT has significantly increased because of the interlocking of the roughened surface with nano-rod-like particles that entered into the voids of the TiO(2) nanotubes. Compared with that of the bare Ti, the corrosion current density of FAgHA/TNT-coated Ti substrate decreased from 3.71 to 0.18 μA, and its corrosion resistance increased by almost two orders of magnitude. Moreover, despite pure HA, the FAgHA killed all viable Staphylococcus aureus after 24 hours of incubation. Although the fabricated FAgHA/TNT coating is hydrophobic, it induced deposition of the typical spherical apatite when immersed in a simulated body fluid (SBF); the osteoblasts spread very well on the surface of the coating. In addition, in vitro cell culture tests demonstrated cell viability and alkaline phosphatase (ALP) similar to pure HA, which indicated good cytocompatibility. Interestingly, compared with bare Ti, FAgHA/TNT-coated Ti surface was innocent for cell vitality and even more beneficial for cell osteogenesis in vitro. CONCLUSION: Enhancing the osseointegration and preventing infection in implants, the FAgHA/TNT-coated Ti makes implants more successful. Dove Medical Press 2018-05-03 /pmc/articles/PMC5937497/ /pubmed/29760549 http://dx.doi.org/10.2147/IJN.S162558 Text en © 2018 Huang et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Huang, Yong Song, Guiqin Chang, Xiaotong Wang, Zhenhui Zhang, Xuejiao Han, Shuguang Su, Zhuobin Yang, Hejie Yang, Dongdong Zhang, Xiaojun Nanostructured Ag(+)-substituted fluorhydroxyapatite-TiO(2) coatings for enhanced bactericidal effects and osteoinductivity of Ti for biomedical applications |
title | Nanostructured Ag(+)-substituted fluorhydroxyapatite-TiO(2) coatings for enhanced bactericidal effects and osteoinductivity of Ti for biomedical applications |
title_full | Nanostructured Ag(+)-substituted fluorhydroxyapatite-TiO(2) coatings for enhanced bactericidal effects and osteoinductivity of Ti for biomedical applications |
title_fullStr | Nanostructured Ag(+)-substituted fluorhydroxyapatite-TiO(2) coatings for enhanced bactericidal effects and osteoinductivity of Ti for biomedical applications |
title_full_unstemmed | Nanostructured Ag(+)-substituted fluorhydroxyapatite-TiO(2) coatings for enhanced bactericidal effects and osteoinductivity of Ti for biomedical applications |
title_short | Nanostructured Ag(+)-substituted fluorhydroxyapatite-TiO(2) coatings for enhanced bactericidal effects and osteoinductivity of Ti for biomedical applications |
title_sort | nanostructured ag(+)-substituted fluorhydroxyapatite-tio(2) coatings for enhanced bactericidal effects and osteoinductivity of ti for biomedical applications |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5937497/ https://www.ncbi.nlm.nih.gov/pubmed/29760549 http://dx.doi.org/10.2147/IJN.S162558 |
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