Cargando…

High-throughput evaluation of T7 promoter variants using biased randomization and DNA barcoding

Cis-regulatory elements (CREs) are one of the important factors in controlling gene expression and elucidation of their roles has been attracting great interest. We have developed an improved method for analyzing a large variety of mutant CRE sequences in a simple and high-throughput manner. In our...

Descripción completa

Detalles Bibliográficos
Autores principales: Komura, Ryo, Aoki, Wataru, Motone, Keisuke, Satomura, Atsushi, Ueda, Mitsuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5937735/
https://www.ncbi.nlm.nih.gov/pubmed/29734387
http://dx.doi.org/10.1371/journal.pone.0196905