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High-throughput evaluation of T7 promoter variants using biased randomization and DNA barcoding
Cis-regulatory elements (CREs) are one of the important factors in controlling gene expression and elucidation of their roles has been attracting great interest. We have developed an improved method for analyzing a large variety of mutant CRE sequences in a simple and high-throughput manner. In our...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5937735/ https://www.ncbi.nlm.nih.gov/pubmed/29734387 http://dx.doi.org/10.1371/journal.pone.0196905 |