Cargando…

A guide to (13)C metabolic flux analysis for the cancer biologist

Cancer metabolism is significantly altered from normal cellular metabolism allowing cancer cells to adapt to changing microenvironments and maintain high rates of proliferation. In the past decade, stable-isotope tracing and network analysis have become powerful tools for uncovering metabolic pathwa...

Descripción completa

Detalles Bibliográficos
Autor principal: Antoniewicz, Maciek R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5938039/
https://www.ncbi.nlm.nih.gov/pubmed/29657327
http://dx.doi.org/10.1038/s12276-018-0060-y
_version_ 1783320726349021184
author Antoniewicz, Maciek R.
author_facet Antoniewicz, Maciek R.
author_sort Antoniewicz, Maciek R.
collection PubMed
description Cancer metabolism is significantly altered from normal cellular metabolism allowing cancer cells to adapt to changing microenvironments and maintain high rates of proliferation. In the past decade, stable-isotope tracing and network analysis have become powerful tools for uncovering metabolic pathways that are differentially activated in cancer cells. In particular, (13)C metabolic flux analysis ((13)C-MFA) has emerged as the primary technique for quantifying intracellular fluxes in cancer cells. In this review, we provide a practical guide for investigators interested in getting started with (13)C-MFA. We describe best practices in (13)C-MFA, highlight potential pitfalls and alternative approaches, and conclude with new developments that can further enhance our understanding of cancer metabolism.
format Online
Article
Text
id pubmed-5938039
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-59380392018-05-15 A guide to (13)C metabolic flux analysis for the cancer biologist Antoniewicz, Maciek R. Exp Mol Med Review Article Cancer metabolism is significantly altered from normal cellular metabolism allowing cancer cells to adapt to changing microenvironments and maintain high rates of proliferation. In the past decade, stable-isotope tracing and network analysis have become powerful tools for uncovering metabolic pathways that are differentially activated in cancer cells. In particular, (13)C metabolic flux analysis ((13)C-MFA) has emerged as the primary technique for quantifying intracellular fluxes in cancer cells. In this review, we provide a practical guide for investigators interested in getting started with (13)C-MFA. We describe best practices in (13)C-MFA, highlight potential pitfalls and alternative approaches, and conclude with new developments that can further enhance our understanding of cancer metabolism. Nature Publishing Group UK 2018-04-16 /pmc/articles/PMC5938039/ /pubmed/29657327 http://dx.doi.org/10.1038/s12276-018-0060-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Review Article
Antoniewicz, Maciek R.
A guide to (13)C metabolic flux analysis for the cancer biologist
title A guide to (13)C metabolic flux analysis for the cancer biologist
title_full A guide to (13)C metabolic flux analysis for the cancer biologist
title_fullStr A guide to (13)C metabolic flux analysis for the cancer biologist
title_full_unstemmed A guide to (13)C metabolic flux analysis for the cancer biologist
title_short A guide to (13)C metabolic flux analysis for the cancer biologist
title_sort guide to (13)c metabolic flux analysis for the cancer biologist
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5938039/
https://www.ncbi.nlm.nih.gov/pubmed/29657327
http://dx.doi.org/10.1038/s12276-018-0060-y
work_keys_str_mv AT antoniewiczmaciekr aguideto13cmetabolicfluxanalysisforthecancerbiologist
AT antoniewiczmaciekr guideto13cmetabolicfluxanalysisforthecancerbiologist