The Diagnosis of Gastric Mucosa-associated Lymphoid Tissue Lymphoma by Flow Cytometry and Fluorescence in situ Hybridization of Biopsy Specimens

Mucosa-associated lymphoid tissue (MALT) lymphoma and reactive inflammatory lymphoid changes are frequently difficult to distinguish based on a routine histological differential diagnosis. We were unable to diagnose gastric MALT lymphoma histologically using specimens obtained by endoscopy, although...

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Autores principales: Matsueda, Katsunori, Omote, Sizuma, Sakata, Masahiro, Fujita, Isao, Horii, Jouichiro, Toyokawa, Tatsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Internal Medicine 2017
Materias:
Case Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5938496/
https://www.ncbi.nlm.nih.gov/pubmed/29269666
http://dx.doi.org/10.2169/internalmedicine.9617-17
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author Matsueda, Katsunori
Omote, Sizuma
Sakata, Masahiro
Fujita, Isao
Horii, Jouichiro
Toyokawa, Tatsuya
author_facet Matsueda, Katsunori
Omote, Sizuma
Sakata, Masahiro
Fujita, Isao
Horii, Jouichiro
Toyokawa, Tatsuya
author_sort Matsueda, Katsunori
collection PubMed
description Mucosa-associated lymphoid tissue (MALT) lymphoma and reactive inflammatory lymphoid changes are frequently difficult to distinguish based on a routine histological differential diagnosis. We were unable to diagnose gastric MALT lymphoma histologically using specimens obtained by endoscopy, although a flow cytometry (FCM) analysis demonstrated clonality of neoplastic cells by separating cells by CD45 gating. Furthermore, a fluorescence in situ hybridization (FISH) analysis showed trisomy 18. We therefore diagnosed gastric MALT lymphoma with trisomy 18. We recommend that FCM and FISH analyses of biopsy specimens be considered for diagnosing gastric MALT lymphoma if this diagnosis is suspected based on endoscopic findings.
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spelling pubmed-59384962018-05-08 The Diagnosis of Gastric Mucosa-associated Lymphoid Tissue Lymphoma by Flow Cytometry and Fluorescence in situ Hybridization of Biopsy Specimens Matsueda, Katsunori Omote, Sizuma Sakata, Masahiro Fujita, Isao Horii, Jouichiro Toyokawa, Tatsuya Intern Med Case Report Mucosa-associated lymphoid tissue (MALT) lymphoma and reactive inflammatory lymphoid changes are frequently difficult to distinguish based on a routine histological differential diagnosis. We were unable to diagnose gastric MALT lymphoma histologically using specimens obtained by endoscopy, although a flow cytometry (FCM) analysis demonstrated clonality of neoplastic cells by separating cells by CD45 gating. Furthermore, a fluorescence in situ hybridization (FISH) analysis showed trisomy 18. We therefore diagnosed gastric MALT lymphoma with trisomy 18. We recommend that FCM and FISH analyses of biopsy specimens be considered for diagnosing gastric MALT lymphoma if this diagnosis is suspected based on endoscopic findings. The Japanese Society of Internal Medicine 2017-12-21 2018-04-15 /pmc/articles/PMC5938496/ /pubmed/29269666 http://dx.doi.org/10.2169/internalmedicine.9617-17 Text en Copyright © 2018 by The Japanese Society of Internal Medicine https://creativecommons.org/licenses/by-nc-nd/4.0/ The Internal Medicine is an Open Access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. To view the details of this license, please visit (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Case Report
Matsueda, Katsunori
Omote, Sizuma
Sakata, Masahiro
Fujita, Isao
Horii, Jouichiro
Toyokawa, Tatsuya
The Diagnosis of Gastric Mucosa-associated Lymphoid Tissue Lymphoma by Flow Cytometry and Fluorescence in situ Hybridization of Biopsy Specimens
title The Diagnosis of Gastric Mucosa-associated Lymphoid Tissue Lymphoma by Flow Cytometry and Fluorescence in situ Hybridization of Biopsy Specimens
title_full The Diagnosis of Gastric Mucosa-associated Lymphoid Tissue Lymphoma by Flow Cytometry and Fluorescence in situ Hybridization of Biopsy Specimens
title_fullStr The Diagnosis of Gastric Mucosa-associated Lymphoid Tissue Lymphoma by Flow Cytometry and Fluorescence in situ Hybridization of Biopsy Specimens
title_full_unstemmed The Diagnosis of Gastric Mucosa-associated Lymphoid Tissue Lymphoma by Flow Cytometry and Fluorescence in situ Hybridization of Biopsy Specimens
title_short The Diagnosis of Gastric Mucosa-associated Lymphoid Tissue Lymphoma by Flow Cytometry and Fluorescence in situ Hybridization of Biopsy Specimens
title_sort diagnosis of gastric mucosa-associated lymphoid tissue lymphoma by flow cytometry and fluorescence in situ hybridization of biopsy specimens
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5938496/
https://www.ncbi.nlm.nih.gov/pubmed/29269666
http://dx.doi.org/10.2169/internalmedicine.9617-17
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