Cargando…

MicroRNA-567 inhibits cell proliferation, migration and invasion by targeting FGF5 in osteosarcoma

MicroRNAs (miRNAs) have been widely reported to have important regulatory roles in various human tumors, including osteosarcoma (OS). The aim of this study was to focus on the role of less well-known miRNA-567 (miR-567) in OS. We found the expression of miR-567 was significantly reduced in OS tissue...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Daodong, Zhang, Chaoju, Li, Xiaolin, Zhang, Hongmei, Pang, Qixiong, Wan, An
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Leibniz Research Centre for Working Environment and Human Factors 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5938541/
https://www.ncbi.nlm.nih.gov/pubmed/29743851
http://dx.doi.org/10.17179/excli2017-932
_version_ 1783320805242830848
author Liu, Daodong
Zhang, Chaoju
Li, Xiaolin
Zhang, Hongmei
Pang, Qixiong
Wan, An
author_facet Liu, Daodong
Zhang, Chaoju
Li, Xiaolin
Zhang, Hongmei
Pang, Qixiong
Wan, An
author_sort Liu, Daodong
collection PubMed
description MicroRNAs (miRNAs) have been widely reported to have important regulatory roles in various human tumors, including osteosarcoma (OS). The aim of this study was to focus on the role of less well-known miRNA-567 (miR-567) in OS. We found the expression of miR-567 was significantly reduced in OS tissues and cell lines (MG-63, U2OS and Saos-2) compared with the adjacent normal tissues and normal osteoblastic cells (hFOB), respectively. Moreover, exogenous miR-567 overexpression inhibited OS cell proliferation, migration and invasion by CCK-8, Transwell assays, respectively. We further explored the mechanism underlying the suppressive effects of miR-567 on OS cells and identified a potential target of miR-567 binds to the 3'UTR of fibroblast growth factor 5 (FGF5) using TargetScan program. Furthermore, enforced expression of miR-567 decreased the expression of FGF5 in both MG-63 and U2OS cells using luciferase reporter assay and Western blotting. We also showed that overexpression of FGF5 could partially antagonize the suppressive effects of miR-567 on OS cell proliferation, migration and invasion. Taken together, our data indicated that miR-567 may function as a tumor suppressor by negatively regulating FGF5 and be potential therapeutic targets for the treatment of OS.
format Online
Article
Text
id pubmed-5938541
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Leibniz Research Centre for Working Environment and Human Factors
record_format MEDLINE/PubMed
spelling pubmed-59385412018-05-09 MicroRNA-567 inhibits cell proliferation, migration and invasion by targeting FGF5 in osteosarcoma Liu, Daodong Zhang, Chaoju Li, Xiaolin Zhang, Hongmei Pang, Qixiong Wan, An EXCLI J Original Article MicroRNAs (miRNAs) have been widely reported to have important regulatory roles in various human tumors, including osteosarcoma (OS). The aim of this study was to focus on the role of less well-known miRNA-567 (miR-567) in OS. We found the expression of miR-567 was significantly reduced in OS tissues and cell lines (MG-63, U2OS and Saos-2) compared with the adjacent normal tissues and normal osteoblastic cells (hFOB), respectively. Moreover, exogenous miR-567 overexpression inhibited OS cell proliferation, migration and invasion by CCK-8, Transwell assays, respectively. We further explored the mechanism underlying the suppressive effects of miR-567 on OS cells and identified a potential target of miR-567 binds to the 3'UTR of fibroblast growth factor 5 (FGF5) using TargetScan program. Furthermore, enforced expression of miR-567 decreased the expression of FGF5 in both MG-63 and U2OS cells using luciferase reporter assay and Western blotting. We also showed that overexpression of FGF5 could partially antagonize the suppressive effects of miR-567 on OS cell proliferation, migration and invasion. Taken together, our data indicated that miR-567 may function as a tumor suppressor by negatively regulating FGF5 and be potential therapeutic targets for the treatment of OS. Leibniz Research Centre for Working Environment and Human Factors 2018-01-15 /pmc/articles/PMC5938541/ /pubmed/29743851 http://dx.doi.org/10.17179/excli2017-932 Text en Copyright © 2018 Liu et al. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0/) You are free to copy, distribute and transmit the work, provided the original author and source are credited.
spellingShingle Original Article
Liu, Daodong
Zhang, Chaoju
Li, Xiaolin
Zhang, Hongmei
Pang, Qixiong
Wan, An
MicroRNA-567 inhibits cell proliferation, migration and invasion by targeting FGF5 in osteosarcoma
title MicroRNA-567 inhibits cell proliferation, migration and invasion by targeting FGF5 in osteosarcoma
title_full MicroRNA-567 inhibits cell proliferation, migration and invasion by targeting FGF5 in osteosarcoma
title_fullStr MicroRNA-567 inhibits cell proliferation, migration and invasion by targeting FGF5 in osteosarcoma
title_full_unstemmed MicroRNA-567 inhibits cell proliferation, migration and invasion by targeting FGF5 in osteosarcoma
title_short MicroRNA-567 inhibits cell proliferation, migration and invasion by targeting FGF5 in osteosarcoma
title_sort microrna-567 inhibits cell proliferation, migration and invasion by targeting fgf5 in osteosarcoma
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5938541/
https://www.ncbi.nlm.nih.gov/pubmed/29743851
http://dx.doi.org/10.17179/excli2017-932
work_keys_str_mv AT liudaodong microrna567inhibitscellproliferationmigrationandinvasionbytargetingfgf5inosteosarcoma
AT zhangchaoju microrna567inhibitscellproliferationmigrationandinvasionbytargetingfgf5inosteosarcoma
AT lixiaolin microrna567inhibitscellproliferationmigrationandinvasionbytargetingfgf5inosteosarcoma
AT zhanghongmei microrna567inhibitscellproliferationmigrationandinvasionbytargetingfgf5inosteosarcoma
AT pangqixiong microrna567inhibitscellproliferationmigrationandinvasionbytargetingfgf5inosteosarcoma
AT wanan microrna567inhibitscellproliferationmigrationandinvasionbytargetingfgf5inosteosarcoma