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Pinus Roxburghii essential oil anticancer activity and chemical composition evaluation
The present study was conducted to appraise the anticancer activity of Pinus roxburghii essential oil along with chemical composition evaluation. MTT assay revealed cytotoxicity induction in colon, leukemia, multiple myeloma, pancreatic, head and neck and lung cancer cells exposed to essential oil....
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Leibniz Research Centre for Working Environment and Human Factors
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5938542/ https://www.ncbi.nlm.nih.gov/pubmed/29743861 http://dx.doi.org/10.17179/excli2016-670 |
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author | Sajid, Arfaa Manzoor, Qaisar Iqbal, Munawar Tyagi, Amit Kumar Sarfraz, Raja Adil Sajid, Anam |
author_facet | Sajid, Arfaa Manzoor, Qaisar Iqbal, Munawar Tyagi, Amit Kumar Sarfraz, Raja Adil Sajid, Anam |
author_sort | Sajid, Arfaa |
collection | PubMed |
description | The present study was conducted to appraise the anticancer activity of Pinus roxburghii essential oil along with chemical composition evaluation. MTT assay revealed cytotoxicity induction in colon, leukemia, multiple myeloma, pancreatic, head and neck and lung cancer cells exposed to essential oil. Cancer cell death was also observed through live/dead cell viability assay and FACS analysis. Apoptosis induced by essential oil was confirmed by cleavage of PARP and caspase-3 that suppressed the colony-forming ability of tumor cells and 50 % inhibition occurred at a dose of 25 μg/mL. Moreover, essential oil inhibited the activation of inflammatory transcription factor NF-κB and inhibited expression of NF-κB regulated gene products linked to cell survival (survivin, c-FLIP, Bcl-2, Bcl-xL, c-Myc, c-IAP2), proliferation (Cyclin D1) and metastasis (MMP-9). P. roxburghii essential oil has considerable anticancer activity and could be used as anticancer agent, which needs further investigation to identify and purify the bioactive compounds followed by in vivo studies. |
format | Online Article Text |
id | pubmed-5938542 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Leibniz Research Centre for Working Environment and Human Factors |
record_format | MEDLINE/PubMed |
spelling | pubmed-59385422018-05-09 Pinus Roxburghii essential oil anticancer activity and chemical composition evaluation Sajid, Arfaa Manzoor, Qaisar Iqbal, Munawar Tyagi, Amit Kumar Sarfraz, Raja Adil Sajid, Anam EXCLI J Original Article The present study was conducted to appraise the anticancer activity of Pinus roxburghii essential oil along with chemical composition evaluation. MTT assay revealed cytotoxicity induction in colon, leukemia, multiple myeloma, pancreatic, head and neck and lung cancer cells exposed to essential oil. Cancer cell death was also observed through live/dead cell viability assay and FACS analysis. Apoptosis induced by essential oil was confirmed by cleavage of PARP and caspase-3 that suppressed the colony-forming ability of tumor cells and 50 % inhibition occurred at a dose of 25 μg/mL. Moreover, essential oil inhibited the activation of inflammatory transcription factor NF-κB and inhibited expression of NF-κB regulated gene products linked to cell survival (survivin, c-FLIP, Bcl-2, Bcl-xL, c-Myc, c-IAP2), proliferation (Cyclin D1) and metastasis (MMP-9). P. roxburghii essential oil has considerable anticancer activity and could be used as anticancer agent, which needs further investigation to identify and purify the bioactive compounds followed by in vivo studies. Leibniz Research Centre for Working Environment and Human Factors 2018-03-12 /pmc/articles/PMC5938542/ /pubmed/29743861 http://dx.doi.org/10.17179/excli2016-670 Text en Copyright © 2018 Sajid et al. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0/) You are free to copy, distribute and transmit the work, provided the original author and source are credited. |
spellingShingle | Original Article Sajid, Arfaa Manzoor, Qaisar Iqbal, Munawar Tyagi, Amit Kumar Sarfraz, Raja Adil Sajid, Anam Pinus Roxburghii essential oil anticancer activity and chemical composition evaluation |
title | Pinus Roxburghii essential oil anticancer activity and chemical composition evaluation |
title_full | Pinus Roxburghii essential oil anticancer activity and chemical composition evaluation |
title_fullStr | Pinus Roxburghii essential oil anticancer activity and chemical composition evaluation |
title_full_unstemmed | Pinus Roxburghii essential oil anticancer activity and chemical composition evaluation |
title_short | Pinus Roxburghii essential oil anticancer activity and chemical composition evaluation |
title_sort | pinus roxburghii essential oil anticancer activity and chemical composition evaluation |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5938542/ https://www.ncbi.nlm.nih.gov/pubmed/29743861 http://dx.doi.org/10.17179/excli2016-670 |
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