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Generation of human iPSCs from urine derived cells of patient with a novel heterozygous PAI-1 mutation
We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subjects heterozygous for a novel Plasminogen Activator Inhibitor-1 (PAI-1) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanak...
| Autores principales: | , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5939958/ https://www.ncbi.nlm.nih.gov/pubmed/28395801 http://dx.doi.org/10.1016/j.scr.2016.12.003 |
| _version_ | 1783321028539187200 |
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| author | Afzal, Muhammad Zeeshan Gartz, Melanie Klyachko, Ekaterina A. Khan, Sadiya Sana Shah, Sanjiv J. Gupta, Sweta Shapiro, Amy D. Vaughan, Douglas E. Strande, Jennifer L. |
| author_facet | Afzal, Muhammad Zeeshan Gartz, Melanie Klyachko, Ekaterina A. Khan, Sadiya Sana Shah, Sanjiv J. Gupta, Sweta Shapiro, Amy D. Vaughan, Douglas E. Strande, Jennifer L. |
| author_sort | Afzal, Muhammad Zeeshan |
| collection | PubMed |
| description | We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subjects heterozygous for a novel Plasminogen Activator Inhibitor-1 (PAI-1) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the PAI-1 UCs. |
| format | Online Article Text |
| id | pubmed-5939958 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2016 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-59399582018-05-08 Generation of human iPSCs from urine derived cells of patient with a novel heterozygous PAI-1 mutation Afzal, Muhammad Zeeshan Gartz, Melanie Klyachko, Ekaterina A. Khan, Sadiya Sana Shah, Sanjiv J. Gupta, Sweta Shapiro, Amy D. Vaughan, Douglas E. Strande, Jennifer L. Stem Cell Res Article We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subjects heterozygous for a novel Plasminogen Activator Inhibitor-1 (PAI-1) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the PAI-1 UCs. 2016-12-09 2017-01 /pmc/articles/PMC5939958/ /pubmed/28395801 http://dx.doi.org/10.1016/j.scr.2016.12.003 Text en This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| spellingShingle | Article Afzal, Muhammad Zeeshan Gartz, Melanie Klyachko, Ekaterina A. Khan, Sadiya Sana Shah, Sanjiv J. Gupta, Sweta Shapiro, Amy D. Vaughan, Douglas E. Strande, Jennifer L. Generation of human iPSCs from urine derived cells of patient with a novel heterozygous PAI-1 mutation |
| title | Generation of human iPSCs from urine derived cells of patient with a novel heterozygous PAI-1 mutation |
| title_full | Generation of human iPSCs from urine derived cells of patient with a novel heterozygous PAI-1 mutation |
| title_fullStr | Generation of human iPSCs from urine derived cells of patient with a novel heterozygous PAI-1 mutation |
| title_full_unstemmed | Generation of human iPSCs from urine derived cells of patient with a novel heterozygous PAI-1 mutation |
| title_short | Generation of human iPSCs from urine derived cells of patient with a novel heterozygous PAI-1 mutation |
| title_sort | generation of human ipscs from urine derived cells of patient with a novel heterozygous pai-1 mutation |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5939958/ https://www.ncbi.nlm.nih.gov/pubmed/28395801 http://dx.doi.org/10.1016/j.scr.2016.12.003 |
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