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Cold Shock as a Screen for Genes Involved in Cold Acclimatization in Neurospora crassa
When subjected to rapid drops of temperature (cold shock), Neurospora responds with a temporary shift in its morphology. This report is the first to examine this response genetically. We report here the results of a screen of selected mutants from the Neurospora knockout library for alterations in t...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Genetics Society of America
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5940138/ https://www.ncbi.nlm.nih.gov/pubmed/29563189 http://dx.doi.org/10.1534/g3.118.200112 |
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author | Watters, Michael K. Manzanilla, Victor Howell, Holly Mehreteab, Alexander Rose, Erik Walters, Nicole Seitz, Nicholas Nava, Jacob Kekelik, Sienna Knuth, Laura Scivinsky, Brianna |
author_facet | Watters, Michael K. Manzanilla, Victor Howell, Holly Mehreteab, Alexander Rose, Erik Walters, Nicole Seitz, Nicholas Nava, Jacob Kekelik, Sienna Knuth, Laura Scivinsky, Brianna |
author_sort | Watters, Michael K. |
collection | PubMed |
description | When subjected to rapid drops of temperature (cold shock), Neurospora responds with a temporary shift in its morphology. This report is the first to examine this response genetically. We report here the results of a screen of selected mutants from the Neurospora knockout library for alterations in their morphological response to cold shock. Three groups of knockouts were selected to be subject to this screen: genes previously suspected to be involved in hyphal development as well as knockouts resulting in morphological changes; transcription factors; and genes homologous to E. coli genes known to alter their expression in response to cold shock. A total of 344 knockout strains were subjected to cold shock. Of those, 118 strains were identified with altered responses. We report here the cold shock morphologies and GO categorizations of strains subjected to this screen. Of strains with knockouts in genes associated with hyphal growth or morphology, 33 of 131 tested (25%) showed an altered response to cold shock. Of strains with knockouts in transcription factor genes, 30 of 145 (20%) showed an altered response to cold shock. Of strains with knockouts in genes homologous to E. coli genes which display altered levels of transcription in response to cold shock, a total of 55 of 68 tested (81%) showed an altered cold shock response. This suggests that the response to cold shock in these two organisms is largely shared in common. |
format | Online Article Text |
id | pubmed-5940138 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Genetics Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-59401382018-05-10 Cold Shock as a Screen for Genes Involved in Cold Acclimatization in Neurospora crassa Watters, Michael K. Manzanilla, Victor Howell, Holly Mehreteab, Alexander Rose, Erik Walters, Nicole Seitz, Nicholas Nava, Jacob Kekelik, Sienna Knuth, Laura Scivinsky, Brianna G3 (Bethesda) Mutant Screen Report When subjected to rapid drops of temperature (cold shock), Neurospora responds with a temporary shift in its morphology. This report is the first to examine this response genetically. We report here the results of a screen of selected mutants from the Neurospora knockout library for alterations in their morphological response to cold shock. Three groups of knockouts were selected to be subject to this screen: genes previously suspected to be involved in hyphal development as well as knockouts resulting in morphological changes; transcription factors; and genes homologous to E. coli genes known to alter their expression in response to cold shock. A total of 344 knockout strains were subjected to cold shock. Of those, 118 strains were identified with altered responses. We report here the cold shock morphologies and GO categorizations of strains subjected to this screen. Of strains with knockouts in genes associated with hyphal growth or morphology, 33 of 131 tested (25%) showed an altered response to cold shock. Of strains with knockouts in transcription factor genes, 30 of 145 (20%) showed an altered response to cold shock. Of strains with knockouts in genes homologous to E. coli genes which display altered levels of transcription in response to cold shock, a total of 55 of 68 tested (81%) showed an altered cold shock response. This suggests that the response to cold shock in these two organisms is largely shared in common. Genetics Society of America 2018-03-21 /pmc/articles/PMC5940138/ /pubmed/29563189 http://dx.doi.org/10.1534/g3.118.200112 Text en Copyright © 2018 Watters et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Mutant Screen Report Watters, Michael K. Manzanilla, Victor Howell, Holly Mehreteab, Alexander Rose, Erik Walters, Nicole Seitz, Nicholas Nava, Jacob Kekelik, Sienna Knuth, Laura Scivinsky, Brianna Cold Shock as a Screen for Genes Involved in Cold Acclimatization in Neurospora crassa |
title | Cold Shock as a Screen for Genes Involved in Cold Acclimatization in Neurospora crassa |
title_full | Cold Shock as a Screen for Genes Involved in Cold Acclimatization in Neurospora crassa |
title_fullStr | Cold Shock as a Screen for Genes Involved in Cold Acclimatization in Neurospora crassa |
title_full_unstemmed | Cold Shock as a Screen for Genes Involved in Cold Acclimatization in Neurospora crassa |
title_short | Cold Shock as a Screen for Genes Involved in Cold Acclimatization in Neurospora crassa |
title_sort | cold shock as a screen for genes involved in cold acclimatization in neurospora crassa |
topic | Mutant Screen Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5940138/ https://www.ncbi.nlm.nih.gov/pubmed/29563189 http://dx.doi.org/10.1534/g3.118.200112 |
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