Cargando…
Simultaneous quantification of serum monounsaturated and polyunsaturated phosphatidylcholines as potential biomarkers for diagnosing non-small cell lung cancer
Non-small cell lung cancer (NSCLC) is one of the most common malignancies worldwide. In this study, we investigated Ultrahigh Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry and Gas Chromatography Time-of-Flight/Mass Spectrometry-based non-targeted metabolomic profiles...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5940703/ https://www.ncbi.nlm.nih.gov/pubmed/29740076 http://dx.doi.org/10.1038/s41598-018-25552-z |
_version_ | 1783321136177610752 |
---|---|
author | Chen, Yingrong Ma, Zhihong Zhong, Jing Li, Liqin Min, Lishan Xu, Limin Li, Hongwei Zhang, Jianbin Wu, Wei Dai, Licheng |
author_facet | Chen, Yingrong Ma, Zhihong Zhong, Jing Li, Liqin Min, Lishan Xu, Limin Li, Hongwei Zhang, Jianbin Wu, Wei Dai, Licheng |
author_sort | Chen, Yingrong |
collection | PubMed |
description | Non-small cell lung cancer (NSCLC) is one of the most common malignancies worldwide. In this study, we investigated Ultrahigh Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry and Gas Chromatography Time-of-Flight/Mass Spectrometry-based non-targeted metabolomic profiles of serum samples obtained from early-stage NSCLC patients and healthy controls (HC). Metabolic pathways and the biological relevance of potential biomarkers were extensively studied to gain insights into dysregulated metabolism in NSCLC. The identified biomarker candidates were further externally validated via a targeted metabolomics analysis. The global metabolomics profiles could clearly distinguish NSCLC patients from HC. Phosphatidylcholine (PC) levels were found to be dysregulated in glycerophospholipid (GPL) metabolism, which was the top altered pathway in early-stage NSCLC. Compared with those in HC, significant increases in the levels of saturated and monounsaturated PCs such as PC (15:0/18:1), PC (18:0/16:0) and PC (18:0/20:1) were observed in NSCLC. Additionally, relative to those in HC, the levels of 9 polyunsaturated PCs, namely, PC (17:2/2:0), PC (18:4/3:0), and PC (15:0/18:2), and so on were significantly decreased in NSCLC patients. A panel of 12 altered PCs had good diagnostic performance in differentiating early-stage NSCLC patients from HC, and these PCs may thus be used as serum biomarkers for the early diagnosis of NSCLC. |
format | Online Article Text |
id | pubmed-5940703 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59407032018-05-11 Simultaneous quantification of serum monounsaturated and polyunsaturated phosphatidylcholines as potential biomarkers for diagnosing non-small cell lung cancer Chen, Yingrong Ma, Zhihong Zhong, Jing Li, Liqin Min, Lishan Xu, Limin Li, Hongwei Zhang, Jianbin Wu, Wei Dai, Licheng Sci Rep Article Non-small cell lung cancer (NSCLC) is one of the most common malignancies worldwide. In this study, we investigated Ultrahigh Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry and Gas Chromatography Time-of-Flight/Mass Spectrometry-based non-targeted metabolomic profiles of serum samples obtained from early-stage NSCLC patients and healthy controls (HC). Metabolic pathways and the biological relevance of potential biomarkers were extensively studied to gain insights into dysregulated metabolism in NSCLC. The identified biomarker candidates were further externally validated via a targeted metabolomics analysis. The global metabolomics profiles could clearly distinguish NSCLC patients from HC. Phosphatidylcholine (PC) levels were found to be dysregulated in glycerophospholipid (GPL) metabolism, which was the top altered pathway in early-stage NSCLC. Compared with those in HC, significant increases in the levels of saturated and monounsaturated PCs such as PC (15:0/18:1), PC (18:0/16:0) and PC (18:0/20:1) were observed in NSCLC. Additionally, relative to those in HC, the levels of 9 polyunsaturated PCs, namely, PC (17:2/2:0), PC (18:4/3:0), and PC (15:0/18:2), and so on were significantly decreased in NSCLC patients. A panel of 12 altered PCs had good diagnostic performance in differentiating early-stage NSCLC patients from HC, and these PCs may thus be used as serum biomarkers for the early diagnosis of NSCLC. Nature Publishing Group UK 2018-05-08 /pmc/articles/PMC5940703/ /pubmed/29740076 http://dx.doi.org/10.1038/s41598-018-25552-z Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Chen, Yingrong Ma, Zhihong Zhong, Jing Li, Liqin Min, Lishan Xu, Limin Li, Hongwei Zhang, Jianbin Wu, Wei Dai, Licheng Simultaneous quantification of serum monounsaturated and polyunsaturated phosphatidylcholines as potential biomarkers for diagnosing non-small cell lung cancer |
title | Simultaneous quantification of serum monounsaturated and polyunsaturated phosphatidylcholines as potential biomarkers for diagnosing non-small cell lung cancer |
title_full | Simultaneous quantification of serum monounsaturated and polyunsaturated phosphatidylcholines as potential biomarkers for diagnosing non-small cell lung cancer |
title_fullStr | Simultaneous quantification of serum monounsaturated and polyunsaturated phosphatidylcholines as potential biomarkers for diagnosing non-small cell lung cancer |
title_full_unstemmed | Simultaneous quantification of serum monounsaturated and polyunsaturated phosphatidylcholines as potential biomarkers for diagnosing non-small cell lung cancer |
title_short | Simultaneous quantification of serum monounsaturated and polyunsaturated phosphatidylcholines as potential biomarkers for diagnosing non-small cell lung cancer |
title_sort | simultaneous quantification of serum monounsaturated and polyunsaturated phosphatidylcholines as potential biomarkers for diagnosing non-small cell lung cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5940703/ https://www.ncbi.nlm.nih.gov/pubmed/29740076 http://dx.doi.org/10.1038/s41598-018-25552-z |
work_keys_str_mv | AT chenyingrong simultaneousquantificationofserummonounsaturatedandpolyunsaturatedphosphatidylcholinesaspotentialbiomarkersfordiagnosingnonsmallcelllungcancer AT mazhihong simultaneousquantificationofserummonounsaturatedandpolyunsaturatedphosphatidylcholinesaspotentialbiomarkersfordiagnosingnonsmallcelllungcancer AT zhongjing simultaneousquantificationofserummonounsaturatedandpolyunsaturatedphosphatidylcholinesaspotentialbiomarkersfordiagnosingnonsmallcelllungcancer AT liliqin simultaneousquantificationofserummonounsaturatedandpolyunsaturatedphosphatidylcholinesaspotentialbiomarkersfordiagnosingnonsmallcelllungcancer AT minlishan simultaneousquantificationofserummonounsaturatedandpolyunsaturatedphosphatidylcholinesaspotentialbiomarkersfordiagnosingnonsmallcelllungcancer AT xulimin simultaneousquantificationofserummonounsaturatedandpolyunsaturatedphosphatidylcholinesaspotentialbiomarkersfordiagnosingnonsmallcelllungcancer AT lihongwei simultaneousquantificationofserummonounsaturatedandpolyunsaturatedphosphatidylcholinesaspotentialbiomarkersfordiagnosingnonsmallcelllungcancer AT zhangjianbin simultaneousquantificationofserummonounsaturatedandpolyunsaturatedphosphatidylcholinesaspotentialbiomarkersfordiagnosingnonsmallcelllungcancer AT wuwei simultaneousquantificationofserummonounsaturatedandpolyunsaturatedphosphatidylcholinesaspotentialbiomarkersfordiagnosingnonsmallcelllungcancer AT dailicheng simultaneousquantificationofserummonounsaturatedandpolyunsaturatedphosphatidylcholinesaspotentialbiomarkersfordiagnosingnonsmallcelllungcancer |