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Different patterns of epileptiform-like activity are generated in the sclerotic hippocampus from patients with drug-resistant temporal lobe epilepsy

Human hippocampal slice preparations from patients with temporal lobe epilepsy (TLE) associated with hippocampal sclerosis (HS) are excellent material for the characterization of epileptiform-like activity. However, it is still unknown if hippocampal regions as cornu Ammonis (CA) 1, CA3 and CA4, gen...

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Autores principales: Reyes-Garcia, Selvin Z., Scorza, Carla A., Araújo, Noemi S., Ortiz-Villatoro, Nancy N., Jardim, Anaclara Prada, Centeno, Ricardo, Yacubian, Elza Márcia Targas, Faber, Jean, Cavalheiro, Esper A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5940759/
https://www.ncbi.nlm.nih.gov/pubmed/29740014
http://dx.doi.org/10.1038/s41598-018-25378-9
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author Reyes-Garcia, Selvin Z.
Scorza, Carla A.
Araújo, Noemi S.
Ortiz-Villatoro, Nancy N.
Jardim, Anaclara Prada
Centeno, Ricardo
Yacubian, Elza Márcia Targas
Faber, Jean
Cavalheiro, Esper A.
author_facet Reyes-Garcia, Selvin Z.
Scorza, Carla A.
Araújo, Noemi S.
Ortiz-Villatoro, Nancy N.
Jardim, Anaclara Prada
Centeno, Ricardo
Yacubian, Elza Márcia Targas
Faber, Jean
Cavalheiro, Esper A.
author_sort Reyes-Garcia, Selvin Z.
collection PubMed
description Human hippocampal slice preparations from patients with temporal lobe epilepsy (TLE) associated with hippocampal sclerosis (HS) are excellent material for the characterization of epileptiform-like activity. However, it is still unknown if hippocampal regions as cornu Ammonis (CA) 1, CA3 and CA4, generate population epileptiform-like activity. Here, we investigated epileptiform activities of the subiculum, CA1, CA2, CA3, CA4 (induced by elevation of extracellular potassium concentration) and the dentate gyrus (induced with hilar stimulation and elevation of potassium concentration) from sclerotic hippocampi of patients with drug-resistant TLE. Five types of epileptiform-like activity were observed: interictal-like events; periodic ictal spiking; seizure-like events; spreading depression-like events; tonic seizure-like events and no activity. Different susceptibilities to generate epileptiform activity among hippocampal regions were observed; the dentate gyrus was the most susceptible region followed by the subiculum, CA4, CA1, CA2 and CA3. The incidence of epileptiform activity pattern was associated with specific regions of the hippocampal formation. Moreover, it was observed that each region of the hippocampal formation exhibits frequency-specific ranges in each subfield of the sclerotic human tissue. In conclusion, this study demonstrates that epileptiform-like activity may be induced in different regions of the hippocampal formation, including regions that are severely affected by neuronal loss.
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spelling pubmed-59407592018-05-11 Different patterns of epileptiform-like activity are generated in the sclerotic hippocampus from patients with drug-resistant temporal lobe epilepsy Reyes-Garcia, Selvin Z. Scorza, Carla A. Araújo, Noemi S. Ortiz-Villatoro, Nancy N. Jardim, Anaclara Prada Centeno, Ricardo Yacubian, Elza Márcia Targas Faber, Jean Cavalheiro, Esper A. Sci Rep Article Human hippocampal slice preparations from patients with temporal lobe epilepsy (TLE) associated with hippocampal sclerosis (HS) are excellent material for the characterization of epileptiform-like activity. However, it is still unknown if hippocampal regions as cornu Ammonis (CA) 1, CA3 and CA4, generate population epileptiform-like activity. Here, we investigated epileptiform activities of the subiculum, CA1, CA2, CA3, CA4 (induced by elevation of extracellular potassium concentration) and the dentate gyrus (induced with hilar stimulation and elevation of potassium concentration) from sclerotic hippocampi of patients with drug-resistant TLE. Five types of epileptiform-like activity were observed: interictal-like events; periodic ictal spiking; seizure-like events; spreading depression-like events; tonic seizure-like events and no activity. Different susceptibilities to generate epileptiform activity among hippocampal regions were observed; the dentate gyrus was the most susceptible region followed by the subiculum, CA4, CA1, CA2 and CA3. The incidence of epileptiform activity pattern was associated with specific regions of the hippocampal formation. Moreover, it was observed that each region of the hippocampal formation exhibits frequency-specific ranges in each subfield of the sclerotic human tissue. In conclusion, this study demonstrates that epileptiform-like activity may be induced in different regions of the hippocampal formation, including regions that are severely affected by neuronal loss. Nature Publishing Group UK 2018-05-08 /pmc/articles/PMC5940759/ /pubmed/29740014 http://dx.doi.org/10.1038/s41598-018-25378-9 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Reyes-Garcia, Selvin Z.
Scorza, Carla A.
Araújo, Noemi S.
Ortiz-Villatoro, Nancy N.
Jardim, Anaclara Prada
Centeno, Ricardo
Yacubian, Elza Márcia Targas
Faber, Jean
Cavalheiro, Esper A.
Different patterns of epileptiform-like activity are generated in the sclerotic hippocampus from patients with drug-resistant temporal lobe epilepsy
title Different patterns of epileptiform-like activity are generated in the sclerotic hippocampus from patients with drug-resistant temporal lobe epilepsy
title_full Different patterns of epileptiform-like activity are generated in the sclerotic hippocampus from patients with drug-resistant temporal lobe epilepsy
title_fullStr Different patterns of epileptiform-like activity are generated in the sclerotic hippocampus from patients with drug-resistant temporal lobe epilepsy
title_full_unstemmed Different patterns of epileptiform-like activity are generated in the sclerotic hippocampus from patients with drug-resistant temporal lobe epilepsy
title_short Different patterns of epileptiform-like activity are generated in the sclerotic hippocampus from patients with drug-resistant temporal lobe epilepsy
title_sort different patterns of epileptiform-like activity are generated in the sclerotic hippocampus from patients with drug-resistant temporal lobe epilepsy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5940759/
https://www.ncbi.nlm.nih.gov/pubmed/29740014
http://dx.doi.org/10.1038/s41598-018-25378-9
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