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Discovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostome
Neuropeptides are diverse and evolutionarily ancient regulators of physiological/behavioural processes in animals. Here we have investigated the evolution and comparative physiology of luqin-type neuropeptide signalling, which has been characterised previously in protostomian invertebrates. Phylogen...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5940834/ https://www.ncbi.nlm.nih.gov/pubmed/29740074 http://dx.doi.org/10.1038/s41598-018-25606-2 |
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author | Yañez-Guerra, Luis Alfonso Delroisse, Jérôme Barreiro-Iglesias, Antón Slade, Susan E. Scrivens, James H. Elphick, Maurice R. |
author_facet | Yañez-Guerra, Luis Alfonso Delroisse, Jérôme Barreiro-Iglesias, Antón Slade, Susan E. Scrivens, James H. Elphick, Maurice R. |
author_sort | Yañez-Guerra, Luis Alfonso |
collection | PubMed |
description | Neuropeptides are diverse and evolutionarily ancient regulators of physiological/behavioural processes in animals. Here we have investigated the evolution and comparative physiology of luqin-type neuropeptide signalling, which has been characterised previously in protostomian invertebrates. Phylogenetic analysis indicates that luqin-type receptors and tachykinin-type receptors are paralogous and probably originated in a common ancestor of the Bilateria. In the deuterostomian lineage, luqin-type signalling has been lost in chordates but interestingly it has been retained in ambulacrarians. Therefore, here we characterised luqin-type signalling for the first time in an ambulacrarian – the starfish Asterias rubens (phylum Echinodermata). A luqin-like neuropeptide with a C-terminal RWamide motif (ArLQ; EEKTRFPKFMRW-NH(2)) was identified as the ligand for two luqin-type receptors in A. rubens, ArLQR1 and ArLQR2. Furthermore, analysis of the expression of the ArLQ precursor using mRNA in situ hybridisation revealed expression in the nervous system, digestive system and locomotory organs (tube feet) and in vitro pharmacology revealed that ArLQ causes dose-dependent relaxation of tube feet. Accordingly, previous studies have revealed that luqin-type signalling regulates feeding and locomotor activity in protostomes. In conclusion, our phylogenetic analysis combined with characterisation of luqin-type signalling in a deuterostome has provided new insights into neuropeptide evolution and function in the animal kingdom. |
format | Online Article Text |
id | pubmed-5940834 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59408342018-05-11 Discovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostome Yañez-Guerra, Luis Alfonso Delroisse, Jérôme Barreiro-Iglesias, Antón Slade, Susan E. Scrivens, James H. Elphick, Maurice R. Sci Rep Article Neuropeptides are diverse and evolutionarily ancient regulators of physiological/behavioural processes in animals. Here we have investigated the evolution and comparative physiology of luqin-type neuropeptide signalling, which has been characterised previously in protostomian invertebrates. Phylogenetic analysis indicates that luqin-type receptors and tachykinin-type receptors are paralogous and probably originated in a common ancestor of the Bilateria. In the deuterostomian lineage, luqin-type signalling has been lost in chordates but interestingly it has been retained in ambulacrarians. Therefore, here we characterised luqin-type signalling for the first time in an ambulacrarian – the starfish Asterias rubens (phylum Echinodermata). A luqin-like neuropeptide with a C-terminal RWamide motif (ArLQ; EEKTRFPKFMRW-NH(2)) was identified as the ligand for two luqin-type receptors in A. rubens, ArLQR1 and ArLQR2. Furthermore, analysis of the expression of the ArLQ precursor using mRNA in situ hybridisation revealed expression in the nervous system, digestive system and locomotory organs (tube feet) and in vitro pharmacology revealed that ArLQ causes dose-dependent relaxation of tube feet. Accordingly, previous studies have revealed that luqin-type signalling regulates feeding and locomotor activity in protostomes. In conclusion, our phylogenetic analysis combined with characterisation of luqin-type signalling in a deuterostome has provided new insights into neuropeptide evolution and function in the animal kingdom. Nature Publishing Group UK 2018-05-08 /pmc/articles/PMC5940834/ /pubmed/29740074 http://dx.doi.org/10.1038/s41598-018-25606-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Yañez-Guerra, Luis Alfonso Delroisse, Jérôme Barreiro-Iglesias, Antón Slade, Susan E. Scrivens, James H. Elphick, Maurice R. Discovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostome |
title | Discovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostome |
title_full | Discovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostome |
title_fullStr | Discovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostome |
title_full_unstemmed | Discovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostome |
title_short | Discovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostome |
title_sort | discovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5940834/ https://www.ncbi.nlm.nih.gov/pubmed/29740074 http://dx.doi.org/10.1038/s41598-018-25606-2 |
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