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Bordetella pertussis isolates vary in their interactions with human complement components

Whooping cough is a re-emerging respiratory tract infection. It has become clear that there is a need for better understanding of protective immune responses and variation between Bordetella pertussis strains to aid the development of improved vaccines. In order to survive in the host, B. pertussis...

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Autores principales: Brookes, Charlotte, Freire-Martin, Irene, Cavell, Breeze, Alexander, Frances, Taylor, Stephen, Persaud, Ruby, Fry, Norman, Preston, Andrew, Diavatopoulos, Dimitri, Gorringe, Andrew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5940884/
https://www.ncbi.nlm.nih.gov/pubmed/29739922
http://dx.doi.org/10.1038/s41426-018-0084-3
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author Brookes, Charlotte
Freire-Martin, Irene
Cavell, Breeze
Alexander, Frances
Taylor, Stephen
Persaud, Ruby
Fry, Norman
Preston, Andrew
Diavatopoulos, Dimitri
Gorringe, Andrew
author_facet Brookes, Charlotte
Freire-Martin, Irene
Cavell, Breeze
Alexander, Frances
Taylor, Stephen
Persaud, Ruby
Fry, Norman
Preston, Andrew
Diavatopoulos, Dimitri
Gorringe, Andrew
author_sort Brookes, Charlotte
collection PubMed
description Whooping cough is a re-emerging respiratory tract infection. It has become clear that there is a need for better understanding of protective immune responses and variation between Bordetella pertussis strains to aid the development of improved vaccines. In order to survive in the host, B. pertussis has evolved mechanisms to evade complement-mediated killing, including the ability to bind complement-regulatory proteins. Here we evaluate the variation in interactions with the complement system among recently isolated strains. Isolates whose genomes appear highly similar and cluster together on a SNP-based dendrogram were found to vary significantly in resistance to complement-mediated killing and in the deposition of C3b/iC3b, C5b-9 and C1 esterase inhibitor (C1-INH). The key role of Vag8 as a receptor for C1-INH was confirmed and its expression was shown to vary in a panel of isolates. A Vag8 knockout mutant showed increased sensitivity to complement-mediated killing. Antibodies in convalescent sera blocked C1-INH binding to B. pertussis and may play an important role in natural immunity.
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spelling pubmed-59408842018-05-09 Bordetella pertussis isolates vary in their interactions with human complement components Brookes, Charlotte Freire-Martin, Irene Cavell, Breeze Alexander, Frances Taylor, Stephen Persaud, Ruby Fry, Norman Preston, Andrew Diavatopoulos, Dimitri Gorringe, Andrew Emerg Microbes Infect Article Whooping cough is a re-emerging respiratory tract infection. It has become clear that there is a need for better understanding of protective immune responses and variation between Bordetella pertussis strains to aid the development of improved vaccines. In order to survive in the host, B. pertussis has evolved mechanisms to evade complement-mediated killing, including the ability to bind complement-regulatory proteins. Here we evaluate the variation in interactions with the complement system among recently isolated strains. Isolates whose genomes appear highly similar and cluster together on a SNP-based dendrogram were found to vary significantly in resistance to complement-mediated killing and in the deposition of C3b/iC3b, C5b-9 and C1 esterase inhibitor (C1-INH). The key role of Vag8 as a receptor for C1-INH was confirmed and its expression was shown to vary in a panel of isolates. A Vag8 knockout mutant showed increased sensitivity to complement-mediated killing. Antibodies in convalescent sera blocked C1-INH binding to B. pertussis and may play an important role in natural immunity. Nature Publishing Group UK 2018-05-09 /pmc/articles/PMC5940884/ /pubmed/29739922 http://dx.doi.org/10.1038/s41426-018-0084-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Brookes, Charlotte
Freire-Martin, Irene
Cavell, Breeze
Alexander, Frances
Taylor, Stephen
Persaud, Ruby
Fry, Norman
Preston, Andrew
Diavatopoulos, Dimitri
Gorringe, Andrew
Bordetella pertussis isolates vary in their interactions with human complement components
title Bordetella pertussis isolates vary in their interactions with human complement components
title_full Bordetella pertussis isolates vary in their interactions with human complement components
title_fullStr Bordetella pertussis isolates vary in their interactions with human complement components
title_full_unstemmed Bordetella pertussis isolates vary in their interactions with human complement components
title_short Bordetella pertussis isolates vary in their interactions with human complement components
title_sort bordetella pertussis isolates vary in their interactions with human complement components
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5940884/
https://www.ncbi.nlm.nih.gov/pubmed/29739922
http://dx.doi.org/10.1038/s41426-018-0084-3
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