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Biofilm formation is not associated with worse outcome in Acinetobacter baumannii bacteraemic pneumonia
The effect of biofilm formation on bacteraemic pneumonia caused by A. baumannii is unknown. We conducted a 4-year multi-center retrospective study to analyze 71 and 202 patients with A. baumannii bacteraemic pneumonia caused by biofilm-forming and non-biofilm-forming isolates, respectively. The clin...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5940913/ https://www.ncbi.nlm.nih.gov/pubmed/29740176 http://dx.doi.org/10.1038/s41598-018-25661-9 |
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author | Wang, Yung-Chih Huang, Tzu-Wen Yang, Ya-Sung Kuo, Shu-Chen Chen, Chung-Ting Liu, Chang-Pan Liu, Yuag-Meng Chen, Te-Li Chang, Feng-Yee Wu, Shih-Hsiung How, Chorng-Kuang Lee, Yi-Tzu |
author_facet | Wang, Yung-Chih Huang, Tzu-Wen Yang, Ya-Sung Kuo, Shu-Chen Chen, Chung-Ting Liu, Chang-Pan Liu, Yuag-Meng Chen, Te-Li Chang, Feng-Yee Wu, Shih-Hsiung How, Chorng-Kuang Lee, Yi-Tzu |
author_sort | Wang, Yung-Chih |
collection | PubMed |
description | The effect of biofilm formation on bacteraemic pneumonia caused by A. baumannii is unknown. We conducted a 4-year multi-center retrospective study to analyze 71 and 202 patients with A. baumannii bacteraemic pneumonia caused by biofilm-forming and non-biofilm-forming isolates, respectively. The clinical features and outcomes of patients were investigated. Biofilm formation was determined by a microtitre plate assay. The antimicrobial susceptibilities of biofilm-associated cells were assessed using the minimum biofilm eradication concentration (MBEC) assay. Whole-genome sequencing was conducted to identify biofilm-associated genes and their promoters. Quantitative reverse transcription polymerase chain reaction was performed to confirm the expression difference of biofilm-associated genes. There was no significant difference in the clinical characteristics or the outcomes between patients infected with biofilm-forming and non-biofilm-forming strains. Compared with non-biofilm-forming isolates, biofilm-forming isolates exhibited lower resistance to most antimicrobials tested, including imipenem, meropenem, ceftazidime, ciprofloxacin and gentamicin; however, the MBEC assay confirmed the increased antibiotic resistance of the biofilm-embedded bacteria. Biofilm-associated genes and their promoters were detected in most isolates, including the non-biofilm-forming strains. Biofilm-forming isolates showed higher levels of expression of the biofilm-associated genes than non-biofilm-forming isolates. The biofilm-forming ability of A. baumannii isolates might not be associated with worse outcomes in patients with bacteraemic pneumonia. |
format | Online Article Text |
id | pubmed-5940913 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59409132018-05-14 Biofilm formation is not associated with worse outcome in Acinetobacter baumannii bacteraemic pneumonia Wang, Yung-Chih Huang, Tzu-Wen Yang, Ya-Sung Kuo, Shu-Chen Chen, Chung-Ting Liu, Chang-Pan Liu, Yuag-Meng Chen, Te-Li Chang, Feng-Yee Wu, Shih-Hsiung How, Chorng-Kuang Lee, Yi-Tzu Sci Rep Article The effect of biofilm formation on bacteraemic pneumonia caused by A. baumannii is unknown. We conducted a 4-year multi-center retrospective study to analyze 71 and 202 patients with A. baumannii bacteraemic pneumonia caused by biofilm-forming and non-biofilm-forming isolates, respectively. The clinical features and outcomes of patients were investigated. Biofilm formation was determined by a microtitre plate assay. The antimicrobial susceptibilities of biofilm-associated cells were assessed using the minimum biofilm eradication concentration (MBEC) assay. Whole-genome sequencing was conducted to identify biofilm-associated genes and their promoters. Quantitative reverse transcription polymerase chain reaction was performed to confirm the expression difference of biofilm-associated genes. There was no significant difference in the clinical characteristics or the outcomes between patients infected with biofilm-forming and non-biofilm-forming strains. Compared with non-biofilm-forming isolates, biofilm-forming isolates exhibited lower resistance to most antimicrobials tested, including imipenem, meropenem, ceftazidime, ciprofloxacin and gentamicin; however, the MBEC assay confirmed the increased antibiotic resistance of the biofilm-embedded bacteria. Biofilm-associated genes and their promoters were detected in most isolates, including the non-biofilm-forming strains. Biofilm-forming isolates showed higher levels of expression of the biofilm-associated genes than non-biofilm-forming isolates. The biofilm-forming ability of A. baumannii isolates might not be associated with worse outcomes in patients with bacteraemic pneumonia. Nature Publishing Group UK 2018-05-08 /pmc/articles/PMC5940913/ /pubmed/29740176 http://dx.doi.org/10.1038/s41598-018-25661-9 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Wang, Yung-Chih Huang, Tzu-Wen Yang, Ya-Sung Kuo, Shu-Chen Chen, Chung-Ting Liu, Chang-Pan Liu, Yuag-Meng Chen, Te-Li Chang, Feng-Yee Wu, Shih-Hsiung How, Chorng-Kuang Lee, Yi-Tzu Biofilm formation is not associated with worse outcome in Acinetobacter baumannii bacteraemic pneumonia |
title | Biofilm formation is not associated with worse outcome in Acinetobacter baumannii bacteraemic pneumonia |
title_full | Biofilm formation is not associated with worse outcome in Acinetobacter baumannii bacteraemic pneumonia |
title_fullStr | Biofilm formation is not associated with worse outcome in Acinetobacter baumannii bacteraemic pneumonia |
title_full_unstemmed | Biofilm formation is not associated with worse outcome in Acinetobacter baumannii bacteraemic pneumonia |
title_short | Biofilm formation is not associated with worse outcome in Acinetobacter baumannii bacteraemic pneumonia |
title_sort | biofilm formation is not associated with worse outcome in acinetobacter baumannii bacteraemic pneumonia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5940913/ https://www.ncbi.nlm.nih.gov/pubmed/29740176 http://dx.doi.org/10.1038/s41598-018-25661-9 |
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