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Vitamin D(3) increased intestinal Na/Pi-IIb and CYP27B1 mRNA level in rats fed low-phosphorus diets
The objective of the study was to determine the role of vitamin D(3) (VD(3)) in regulating adaptation and mechanism of rats to low-phosphorus (P) diets. Rats were assigned to 4 diets containing 0.2%, 0.4%, 0.6%, or 0.8% P consisting of 5 replicate cages with 6 rats per replicate cage and fed for 7 d...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5941033/ https://www.ncbi.nlm.nih.gov/pubmed/29767113 http://dx.doi.org/10.1016/j.aninu.2016.06.001 |
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author | Cao, Manhu Fang, Rejun Chen, Juan He, Jianhua |
author_facet | Cao, Manhu Fang, Rejun Chen, Juan He, Jianhua |
author_sort | Cao, Manhu |
collection | PubMed |
description | The objective of the study was to determine the role of vitamin D(3) (VD(3)) in regulating adaptation and mechanism of rats to low-phosphorus (P) diets. Rats were assigned to 4 diets containing 0.2%, 0.4%, 0.6%, or 0.8% P consisting of 5 replicate cages with 6 rats per replicate cage and fed for 7 days. Four rats from each replicate cage were treated with ethane-1-hydroxy-1,1-diphosphonicacid, tetrasodium salt (EHDP) and 2 rats remained untreated. Twelve hours prior to preparation on d 7, two of the EHDP-treated rats received an intraperitoneal injection of VD(3) [1,25-(OH)(2)D(3)] at 600 ng per kg body weight, while two rats did not receive the injection. Rats that did not receive VD(3) injection had decreased (P < 0.001) P absorption, but injection of VD(3) resulted in increased (P < 0.001) absorption. The effect of VD(3) injection was greater (P < 0.001) for rats fed 0.2% P diet than rats fed 0.8% P diet in ileum. Sodium dependent phosphate cotransporter type Ⅱb (Na/Pi-II b) and 25-hydroxyvitamin D 1-α hydroxylase (CYP27B1) mRNA level showed the same trend with P absorption. Serum concentration of VD(3) and 1α-hydroxylase activity in rats fed 0.2% P diet were lower than those fed 0.8% P diet. The injection of VD(3) increased (P < 0.001) serum concentration of VD(3) and 1α-hydroxylase activity. Thus, VD(3) increased Na/Pi–IIb and CYP27B1 mRNA level and improved serum concentration of VD(3) and 1α-hydroxylase activity in rats fed low-P diets. |
format | Online Article Text |
id | pubmed-5941033 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | KeAi Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-59410332018-05-14 Vitamin D(3) increased intestinal Na/Pi-IIb and CYP27B1 mRNA level in rats fed low-phosphorus diets Cao, Manhu Fang, Rejun Chen, Juan He, Jianhua Anim Nutr Molecular Nutrition The objective of the study was to determine the role of vitamin D(3) (VD(3)) in regulating adaptation and mechanism of rats to low-phosphorus (P) diets. Rats were assigned to 4 diets containing 0.2%, 0.4%, 0.6%, or 0.8% P consisting of 5 replicate cages with 6 rats per replicate cage and fed for 7 days. Four rats from each replicate cage were treated with ethane-1-hydroxy-1,1-diphosphonicacid, tetrasodium salt (EHDP) and 2 rats remained untreated. Twelve hours prior to preparation on d 7, two of the EHDP-treated rats received an intraperitoneal injection of VD(3) [1,25-(OH)(2)D(3)] at 600 ng per kg body weight, while two rats did not receive the injection. Rats that did not receive VD(3) injection had decreased (P < 0.001) P absorption, but injection of VD(3) resulted in increased (P < 0.001) absorption. The effect of VD(3) injection was greater (P < 0.001) for rats fed 0.2% P diet than rats fed 0.8% P diet in ileum. Sodium dependent phosphate cotransporter type Ⅱb (Na/Pi-II b) and 25-hydroxyvitamin D 1-α hydroxylase (CYP27B1) mRNA level showed the same trend with P absorption. Serum concentration of VD(3) and 1α-hydroxylase activity in rats fed 0.2% P diet were lower than those fed 0.8% P diet. The injection of VD(3) increased (P < 0.001) serum concentration of VD(3) and 1α-hydroxylase activity. Thus, VD(3) increased Na/Pi–IIb and CYP27B1 mRNA level and improved serum concentration of VD(3) and 1α-hydroxylase activity in rats fed low-P diets. KeAi Publishing 2016-09 2016-06-17 /pmc/articles/PMC5941033/ /pubmed/29767113 http://dx.doi.org/10.1016/j.aninu.2016.06.001 Text en © 2016, Chinese Association of Animal Science and Veterinary Medicine. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Molecular Nutrition Cao, Manhu Fang, Rejun Chen, Juan He, Jianhua Vitamin D(3) increased intestinal Na/Pi-IIb and CYP27B1 mRNA level in rats fed low-phosphorus diets |
title | Vitamin D(3) increased intestinal Na/Pi-IIb and CYP27B1 mRNA level in rats fed low-phosphorus diets |
title_full | Vitamin D(3) increased intestinal Na/Pi-IIb and CYP27B1 mRNA level in rats fed low-phosphorus diets |
title_fullStr | Vitamin D(3) increased intestinal Na/Pi-IIb and CYP27B1 mRNA level in rats fed low-phosphorus diets |
title_full_unstemmed | Vitamin D(3) increased intestinal Na/Pi-IIb and CYP27B1 mRNA level in rats fed low-phosphorus diets |
title_short | Vitamin D(3) increased intestinal Na/Pi-IIb and CYP27B1 mRNA level in rats fed low-phosphorus diets |
title_sort | vitamin d(3) increased intestinal na/pi-iib and cyp27b1 mrna level in rats fed low-phosphorus diets |
topic | Molecular Nutrition |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5941033/ https://www.ncbi.nlm.nih.gov/pubmed/29767113 http://dx.doi.org/10.1016/j.aninu.2016.06.001 |
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