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Feed supplementation with arginine and zinc on antioxidant status and inflammatory response in challenged weanling piglets
Although supplementing the diet with zinc oxide and arginine is known to improve growth in weanling piglets, the mechanism of action is not well understood. We measured the antioxidant status and inflammatory response in 48 weanling castrated male piglets fed diets supplemented with or without zinc...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5941224/ https://www.ncbi.nlm.nih.gov/pubmed/29767161 http://dx.doi.org/10.1016/j.aninu.2017.06.009 |
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author | Bergeron, Nadia Robert, Claude Guay, Frédéric |
author_facet | Bergeron, Nadia Robert, Claude Guay, Frédéric |
author_sort | Bergeron, Nadia |
collection | PubMed |
description | Although supplementing the diet with zinc oxide and arginine is known to improve growth in weanling piglets, the mechanism of action is not well understood. We measured the antioxidant status and inflammatory response in 48 weanling castrated male piglets fed diets supplemented with or without zinc oxide (2,500 mg Zn oxide per kg) and arginine (1%) starting at the age of 20 days. The animals were injected with lipopolysaccharide (100 μg/kg) on day 5. Half of them received another injection on day 12. Blood samples were taken just before and 6, 24 and 48 h after injection and the mucosa lining the ileum was recovered following euthanizing on days 7 and 14. Zinc supplementation increased reduced and total glutathione (GSH) (reduced and total) during days 5 to 7 and arginine decreased oxidized GSH measured on days 5 and 12 and the ratio of total antioxidant capacity to total oxidative status during days 12 to 14. Zinc decreased plasma malondialdehyde measured on days 5 and 12 and serum haptoglobin measured on day 12 and increased both metallothionein-1 expression and total antioxidant capacity measured in the ileal mucosa on day 14. Tumour necrosis factor α concentration decreased from days 5 to 12 (all effects were significant at P < 0.05). This study shows that the zinc supplement reduced lipid oxidation and lipopolysaccharide-induced inflammation during the post-weaning period, while the arginine supplementation had only a limited effect. |
format | Online Article Text |
id | pubmed-5941224 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | KeAi Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-59412242018-05-14 Feed supplementation with arginine and zinc on antioxidant status and inflammatory response in challenged weanling piglets Bergeron, Nadia Robert, Claude Guay, Frédéric Anim Nutr Swine Nutrition Although supplementing the diet with zinc oxide and arginine is known to improve growth in weanling piglets, the mechanism of action is not well understood. We measured the antioxidant status and inflammatory response in 48 weanling castrated male piglets fed diets supplemented with or without zinc oxide (2,500 mg Zn oxide per kg) and arginine (1%) starting at the age of 20 days. The animals were injected with lipopolysaccharide (100 μg/kg) on day 5. Half of them received another injection on day 12. Blood samples were taken just before and 6, 24 and 48 h after injection and the mucosa lining the ileum was recovered following euthanizing on days 7 and 14. Zinc supplementation increased reduced and total glutathione (GSH) (reduced and total) during days 5 to 7 and arginine decreased oxidized GSH measured on days 5 and 12 and the ratio of total antioxidant capacity to total oxidative status during days 12 to 14. Zinc decreased plasma malondialdehyde measured on days 5 and 12 and serum haptoglobin measured on day 12 and increased both metallothionein-1 expression and total antioxidant capacity measured in the ileal mucosa on day 14. Tumour necrosis factor α concentration decreased from days 5 to 12 (all effects were significant at P < 0.05). This study shows that the zinc supplement reduced lipid oxidation and lipopolysaccharide-induced inflammation during the post-weaning period, while the arginine supplementation had only a limited effect. KeAi Publishing 2017-09 2017-07-13 /pmc/articles/PMC5941224/ /pubmed/29767161 http://dx.doi.org/10.1016/j.aninu.2017.06.009 Text en © 2017, Chinese Association of Animal Science and Veterinary Medicine. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Swine Nutrition Bergeron, Nadia Robert, Claude Guay, Frédéric Feed supplementation with arginine and zinc on antioxidant status and inflammatory response in challenged weanling piglets |
title | Feed supplementation with arginine and zinc on antioxidant status and inflammatory response in challenged weanling piglets |
title_full | Feed supplementation with arginine and zinc on antioxidant status and inflammatory response in challenged weanling piglets |
title_fullStr | Feed supplementation with arginine and zinc on antioxidant status and inflammatory response in challenged weanling piglets |
title_full_unstemmed | Feed supplementation with arginine and zinc on antioxidant status and inflammatory response in challenged weanling piglets |
title_short | Feed supplementation with arginine and zinc on antioxidant status and inflammatory response in challenged weanling piglets |
title_sort | feed supplementation with arginine and zinc on antioxidant status and inflammatory response in challenged weanling piglets |
topic | Swine Nutrition |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5941224/ https://www.ncbi.nlm.nih.gov/pubmed/29767161 http://dx.doi.org/10.1016/j.aninu.2017.06.009 |
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