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High-oleate yeast oil without polyunsaturated fatty acids
BACKGROUND: Oleate-enriched triacylglycerides are well-suited for lubricant applications that require high oxidative stability. Fatty acid carbon chain length and degree of desaturation are key determinants of triacylglyceride properties and the ability to manipulate fatty acid composition in living...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5941336/ https://www.ncbi.nlm.nih.gov/pubmed/29760773 http://dx.doi.org/10.1186/s13068-018-1131-y |
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author | Tsakraklides, Vasiliki Kamineni, Annapurna Consiglio, Andrew L. MacEwen, Kyle Friedlander, Jonathan Blitzblau, Hannah G. Hamilton, Maureen A. Crabtree, Donald V. Su, Austin Afshar, Jonathan Sullivan, John E. LaTouf, W. Greg South, Colin R. Greenhagen, Emily H. Shaw, A. Joe Brevnova, Elena E. |
author_facet | Tsakraklides, Vasiliki Kamineni, Annapurna Consiglio, Andrew L. MacEwen, Kyle Friedlander, Jonathan Blitzblau, Hannah G. Hamilton, Maureen A. Crabtree, Donald V. Su, Austin Afshar, Jonathan Sullivan, John E. LaTouf, W. Greg South, Colin R. Greenhagen, Emily H. Shaw, A. Joe Brevnova, Elena E. |
author_sort | Tsakraklides, Vasiliki |
collection | PubMed |
description | BACKGROUND: Oleate-enriched triacylglycerides are well-suited for lubricant applications that require high oxidative stability. Fatty acid carbon chain length and degree of desaturation are key determinants of triacylglyceride properties and the ability to manipulate fatty acid composition in living organisms is critical to developing a source of bio-based oil tailored to meet specific application requirements. RESULTS: We sought to engineer the oleaginous yeast Yarrowia lipolytica for production of high-oleate triacylglyceride oil. We studied the effect of deletions and overexpressions in the fatty acid and triacylglyceride synthesis pathways to identify modifications that increase oleate levels. Oleic acid accumulation in triacylglycerides was promoted by exchanging the native ∆9 fatty acid desaturase and glycerol-3-phosphate acyltransferase with heterologous enzymes, as well as deletion of the Δ12 fatty acid desaturase and expression of a fatty acid elongase. By combining these engineering steps, we eliminated polyunsaturated fatty acids and created a Y. lipolytica strain that accumulates triglycerides with > 90% oleate content. CONCLUSIONS: High-oleate content and lack of polyunsaturates distinguish this triacylglyceride oil from plant and algal derived oils. Its composition renders the oil suitable for applications that require high oxidative stability and further demonstrates the potential of Y. lipolytica as a producer of tailored lipid profiles. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13068-018-1131-y) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5941336 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-59413362018-05-14 High-oleate yeast oil without polyunsaturated fatty acids Tsakraklides, Vasiliki Kamineni, Annapurna Consiglio, Andrew L. MacEwen, Kyle Friedlander, Jonathan Blitzblau, Hannah G. Hamilton, Maureen A. Crabtree, Donald V. Su, Austin Afshar, Jonathan Sullivan, John E. LaTouf, W. Greg South, Colin R. Greenhagen, Emily H. Shaw, A. Joe Brevnova, Elena E. Biotechnol Biofuels Research BACKGROUND: Oleate-enriched triacylglycerides are well-suited for lubricant applications that require high oxidative stability. Fatty acid carbon chain length and degree of desaturation are key determinants of triacylglyceride properties and the ability to manipulate fatty acid composition in living organisms is critical to developing a source of bio-based oil tailored to meet specific application requirements. RESULTS: We sought to engineer the oleaginous yeast Yarrowia lipolytica for production of high-oleate triacylglyceride oil. We studied the effect of deletions and overexpressions in the fatty acid and triacylglyceride synthesis pathways to identify modifications that increase oleate levels. Oleic acid accumulation in triacylglycerides was promoted by exchanging the native ∆9 fatty acid desaturase and glycerol-3-phosphate acyltransferase with heterologous enzymes, as well as deletion of the Δ12 fatty acid desaturase and expression of a fatty acid elongase. By combining these engineering steps, we eliminated polyunsaturated fatty acids and created a Y. lipolytica strain that accumulates triglycerides with > 90% oleate content. CONCLUSIONS: High-oleate content and lack of polyunsaturates distinguish this triacylglyceride oil from plant and algal derived oils. Its composition renders the oil suitable for applications that require high oxidative stability and further demonstrates the potential of Y. lipolytica as a producer of tailored lipid profiles. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13068-018-1131-y) contains supplementary material, which is available to authorized users. BioMed Central 2018-05-09 /pmc/articles/PMC5941336/ /pubmed/29760773 http://dx.doi.org/10.1186/s13068-018-1131-y Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Tsakraklides, Vasiliki Kamineni, Annapurna Consiglio, Andrew L. MacEwen, Kyle Friedlander, Jonathan Blitzblau, Hannah G. Hamilton, Maureen A. Crabtree, Donald V. Su, Austin Afshar, Jonathan Sullivan, John E. LaTouf, W. Greg South, Colin R. Greenhagen, Emily H. Shaw, A. Joe Brevnova, Elena E. High-oleate yeast oil without polyunsaturated fatty acids |
title | High-oleate yeast oil without polyunsaturated fatty acids |
title_full | High-oleate yeast oil without polyunsaturated fatty acids |
title_fullStr | High-oleate yeast oil without polyunsaturated fatty acids |
title_full_unstemmed | High-oleate yeast oil without polyunsaturated fatty acids |
title_short | High-oleate yeast oil without polyunsaturated fatty acids |
title_sort | high-oleate yeast oil without polyunsaturated fatty acids |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5941336/ https://www.ncbi.nlm.nih.gov/pubmed/29760773 http://dx.doi.org/10.1186/s13068-018-1131-y |
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