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LncRNA PVT1 promotes the growth of HPV positive and negative cervical squamous cell carcinoma by inhibiting TGF-β1

BACKGROUND: Our study aimed to investigate the role of lncRNA PVT1 in cervical squamous cell carcinoma. MATERIALS AND METHODS: A total of 156 patients with cervical squamous cell carcinoma were enrolled in this study and human papillomavirus (HPV) infection was detected by highly sensitive PCR techn...

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Autores principales: Wang, Xiuli, Wang, Guichan, Zhang, Lijuan, Cong, Jianglin, Hou, Jianqing, Liu, Chunyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5941374/
https://www.ncbi.nlm.nih.gov/pubmed/29760583
http://dx.doi.org/10.1186/s12935-018-0567-2
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author Wang, Xiuli
Wang, Guichan
Zhang, Lijuan
Cong, Jianglin
Hou, Jianqing
Liu, Chunyan
author_facet Wang, Xiuli
Wang, Guichan
Zhang, Lijuan
Cong, Jianglin
Hou, Jianqing
Liu, Chunyan
author_sort Wang, Xiuli
collection PubMed
description BACKGROUND: Our study aimed to investigate the role of lncRNA PVT1 in cervical squamous cell carcinoma. MATERIALS AND METHODS: A total of 156 patients with cervical squamous cell carcinoma were enrolled in this study and human papillomavirus (HPV) infection was detected by highly sensitive PCR techniques. Serum levels of PVT1 in patients infected with different HPVs and healthy controls was detected by qRT-PCR and compared. Serum levels of PVT1 were also compared among patients with different sizes of tumor. ROC curve analysis was performed to evaluate the diagnostic values of serum for cervical squamous cell carcinoma. Survival curves were plotted by Kaplan–Meier method and compared to evaluate the prognostic values of serum PVT1 for this disease. Effects of PVT1 siRNA silencing and overexpression on proliferation of cervical squamous cell carcinoma cells were explored by CCK-8 assay. Western blot was performed to detect the expression of TGF-β1 after PVT1 siRNA silencing and overexpression. RESULTS: No significant differences in serum levels of PVT1 were detected among patients infected with different HPVs and HPV-negative patients. However, serum levels of PVT1 were significantly higher in all patient groups than in healthy control group. Serum level of PVT1 increased with the increased sizes of primary tumor. Serum PVT1 accurately predicted the disease and its prognosis. PVT1 siRNA silencing inhibited the proliferation of cancer cells and reduced the expression of TGF-β1, while PVT1 overexpression played an opposite role. CONCLUSION: LncRNA PVT1 promotes the growth HPV positive and negative cervical squamous cell carcinoma by inhibiting TGF-β1.
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spelling pubmed-59413742018-05-14 LncRNA PVT1 promotes the growth of HPV positive and negative cervical squamous cell carcinoma by inhibiting TGF-β1 Wang, Xiuli Wang, Guichan Zhang, Lijuan Cong, Jianglin Hou, Jianqing Liu, Chunyan Cancer Cell Int Primary Research BACKGROUND: Our study aimed to investigate the role of lncRNA PVT1 in cervical squamous cell carcinoma. MATERIALS AND METHODS: A total of 156 patients with cervical squamous cell carcinoma were enrolled in this study and human papillomavirus (HPV) infection was detected by highly sensitive PCR techniques. Serum levels of PVT1 in patients infected with different HPVs and healthy controls was detected by qRT-PCR and compared. Serum levels of PVT1 were also compared among patients with different sizes of tumor. ROC curve analysis was performed to evaluate the diagnostic values of serum for cervical squamous cell carcinoma. Survival curves were plotted by Kaplan–Meier method and compared to evaluate the prognostic values of serum PVT1 for this disease. Effects of PVT1 siRNA silencing and overexpression on proliferation of cervical squamous cell carcinoma cells were explored by CCK-8 assay. Western blot was performed to detect the expression of TGF-β1 after PVT1 siRNA silencing and overexpression. RESULTS: No significant differences in serum levels of PVT1 were detected among patients infected with different HPVs and HPV-negative patients. However, serum levels of PVT1 were significantly higher in all patient groups than in healthy control group. Serum level of PVT1 increased with the increased sizes of primary tumor. Serum PVT1 accurately predicted the disease and its prognosis. PVT1 siRNA silencing inhibited the proliferation of cancer cells and reduced the expression of TGF-β1, while PVT1 overexpression played an opposite role. CONCLUSION: LncRNA PVT1 promotes the growth HPV positive and negative cervical squamous cell carcinoma by inhibiting TGF-β1. BioMed Central 2018-05-08 /pmc/articles/PMC5941374/ /pubmed/29760583 http://dx.doi.org/10.1186/s12935-018-0567-2 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Primary Research
Wang, Xiuli
Wang, Guichan
Zhang, Lijuan
Cong, Jianglin
Hou, Jianqing
Liu, Chunyan
LncRNA PVT1 promotes the growth of HPV positive and negative cervical squamous cell carcinoma by inhibiting TGF-β1
title LncRNA PVT1 promotes the growth of HPV positive and negative cervical squamous cell carcinoma by inhibiting TGF-β1
title_full LncRNA PVT1 promotes the growth of HPV positive and negative cervical squamous cell carcinoma by inhibiting TGF-β1
title_fullStr LncRNA PVT1 promotes the growth of HPV positive and negative cervical squamous cell carcinoma by inhibiting TGF-β1
title_full_unstemmed LncRNA PVT1 promotes the growth of HPV positive and negative cervical squamous cell carcinoma by inhibiting TGF-β1
title_short LncRNA PVT1 promotes the growth of HPV positive and negative cervical squamous cell carcinoma by inhibiting TGF-β1
title_sort lncrna pvt1 promotes the growth of hpv positive and negative cervical squamous cell carcinoma by inhibiting tgf-β1
topic Primary Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5941374/
https://www.ncbi.nlm.nih.gov/pubmed/29760583
http://dx.doi.org/10.1186/s12935-018-0567-2
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