Cargando…
LncRNA PVT1 promotes the growth of HPV positive and negative cervical squamous cell carcinoma by inhibiting TGF-β1
BACKGROUND: Our study aimed to investigate the role of lncRNA PVT1 in cervical squamous cell carcinoma. MATERIALS AND METHODS: A total of 156 patients with cervical squamous cell carcinoma were enrolled in this study and human papillomavirus (HPV) infection was detected by highly sensitive PCR techn...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5941374/ https://www.ncbi.nlm.nih.gov/pubmed/29760583 http://dx.doi.org/10.1186/s12935-018-0567-2 |
_version_ | 1783321273308282880 |
---|---|
author | Wang, Xiuli Wang, Guichan Zhang, Lijuan Cong, Jianglin Hou, Jianqing Liu, Chunyan |
author_facet | Wang, Xiuli Wang, Guichan Zhang, Lijuan Cong, Jianglin Hou, Jianqing Liu, Chunyan |
author_sort | Wang, Xiuli |
collection | PubMed |
description | BACKGROUND: Our study aimed to investigate the role of lncRNA PVT1 in cervical squamous cell carcinoma. MATERIALS AND METHODS: A total of 156 patients with cervical squamous cell carcinoma were enrolled in this study and human papillomavirus (HPV) infection was detected by highly sensitive PCR techniques. Serum levels of PVT1 in patients infected with different HPVs and healthy controls was detected by qRT-PCR and compared. Serum levels of PVT1 were also compared among patients with different sizes of tumor. ROC curve analysis was performed to evaluate the diagnostic values of serum for cervical squamous cell carcinoma. Survival curves were plotted by Kaplan–Meier method and compared to evaluate the prognostic values of serum PVT1 for this disease. Effects of PVT1 siRNA silencing and overexpression on proliferation of cervical squamous cell carcinoma cells were explored by CCK-8 assay. Western blot was performed to detect the expression of TGF-β1 after PVT1 siRNA silencing and overexpression. RESULTS: No significant differences in serum levels of PVT1 were detected among patients infected with different HPVs and HPV-negative patients. However, serum levels of PVT1 were significantly higher in all patient groups than in healthy control group. Serum level of PVT1 increased with the increased sizes of primary tumor. Serum PVT1 accurately predicted the disease and its prognosis. PVT1 siRNA silencing inhibited the proliferation of cancer cells and reduced the expression of TGF-β1, while PVT1 overexpression played an opposite role. CONCLUSION: LncRNA PVT1 promotes the growth HPV positive and negative cervical squamous cell carcinoma by inhibiting TGF-β1. |
format | Online Article Text |
id | pubmed-5941374 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-59413742018-05-14 LncRNA PVT1 promotes the growth of HPV positive and negative cervical squamous cell carcinoma by inhibiting TGF-β1 Wang, Xiuli Wang, Guichan Zhang, Lijuan Cong, Jianglin Hou, Jianqing Liu, Chunyan Cancer Cell Int Primary Research BACKGROUND: Our study aimed to investigate the role of lncRNA PVT1 in cervical squamous cell carcinoma. MATERIALS AND METHODS: A total of 156 patients with cervical squamous cell carcinoma were enrolled in this study and human papillomavirus (HPV) infection was detected by highly sensitive PCR techniques. Serum levels of PVT1 in patients infected with different HPVs and healthy controls was detected by qRT-PCR and compared. Serum levels of PVT1 were also compared among patients with different sizes of tumor. ROC curve analysis was performed to evaluate the diagnostic values of serum for cervical squamous cell carcinoma. Survival curves were plotted by Kaplan–Meier method and compared to evaluate the prognostic values of serum PVT1 for this disease. Effects of PVT1 siRNA silencing and overexpression on proliferation of cervical squamous cell carcinoma cells were explored by CCK-8 assay. Western blot was performed to detect the expression of TGF-β1 after PVT1 siRNA silencing and overexpression. RESULTS: No significant differences in serum levels of PVT1 were detected among patients infected with different HPVs and HPV-negative patients. However, serum levels of PVT1 were significantly higher in all patient groups than in healthy control group. Serum level of PVT1 increased with the increased sizes of primary tumor. Serum PVT1 accurately predicted the disease and its prognosis. PVT1 siRNA silencing inhibited the proliferation of cancer cells and reduced the expression of TGF-β1, while PVT1 overexpression played an opposite role. CONCLUSION: LncRNA PVT1 promotes the growth HPV positive and negative cervical squamous cell carcinoma by inhibiting TGF-β1. BioMed Central 2018-05-08 /pmc/articles/PMC5941374/ /pubmed/29760583 http://dx.doi.org/10.1186/s12935-018-0567-2 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Primary Research Wang, Xiuli Wang, Guichan Zhang, Lijuan Cong, Jianglin Hou, Jianqing Liu, Chunyan LncRNA PVT1 promotes the growth of HPV positive and negative cervical squamous cell carcinoma by inhibiting TGF-β1 |
title | LncRNA PVT1 promotes the growth of HPV positive and negative cervical squamous cell carcinoma by inhibiting TGF-β1 |
title_full | LncRNA PVT1 promotes the growth of HPV positive and negative cervical squamous cell carcinoma by inhibiting TGF-β1 |
title_fullStr | LncRNA PVT1 promotes the growth of HPV positive and negative cervical squamous cell carcinoma by inhibiting TGF-β1 |
title_full_unstemmed | LncRNA PVT1 promotes the growth of HPV positive and negative cervical squamous cell carcinoma by inhibiting TGF-β1 |
title_short | LncRNA PVT1 promotes the growth of HPV positive and negative cervical squamous cell carcinoma by inhibiting TGF-β1 |
title_sort | lncrna pvt1 promotes the growth of hpv positive and negative cervical squamous cell carcinoma by inhibiting tgf-β1 |
topic | Primary Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5941374/ https://www.ncbi.nlm.nih.gov/pubmed/29760583 http://dx.doi.org/10.1186/s12935-018-0567-2 |
work_keys_str_mv | AT wangxiuli lncrnapvt1promotesthegrowthofhpvpositiveandnegativecervicalsquamouscellcarcinomabyinhibitingtgfb1 AT wangguichan lncrnapvt1promotesthegrowthofhpvpositiveandnegativecervicalsquamouscellcarcinomabyinhibitingtgfb1 AT zhanglijuan lncrnapvt1promotesthegrowthofhpvpositiveandnegativecervicalsquamouscellcarcinomabyinhibitingtgfb1 AT congjianglin lncrnapvt1promotesthegrowthofhpvpositiveandnegativecervicalsquamouscellcarcinomabyinhibitingtgfb1 AT houjianqing lncrnapvt1promotesthegrowthofhpvpositiveandnegativecervicalsquamouscellcarcinomabyinhibitingtgfb1 AT liuchunyan lncrnapvt1promotesthegrowthofhpvpositiveandnegativecervicalsquamouscellcarcinomabyinhibitingtgfb1 |