Characterization and remediation of sample index swaps by non-redundant dual indexing on massively parallel sequencing platforms

BACKGROUND: Here we present an in-depth characterization of the mechanism of sequencer-induced sample contamination due to the phenomenon of index swapping that impacts Illumina sequencers employing patterned flow cells with Exclusion Amplification (ExAmp) chemistry (HiSeqX, HiSeq4000, and NovaSeq)....

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Detalles Bibliográficos
Autores principales: Costello, Maura, Fleharty, Mark, Abreu, Justin, Farjoun, Yossi, Ferriera, Steven, Holmes, Laurie, Granger, Brian, Green, Lisa, Howd, Tom, Mason, Tamara, Vicente, Gina, Dasilva, Michael, Brodeur, Wendy, DeSmet, Timothy, Dodge, Sheila, Lennon, Niall J., Gabriel, Stacey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5941783/
https://www.ncbi.nlm.nih.gov/pubmed/29739332
http://dx.doi.org/10.1186/s12864-018-4703-0

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