Pressurized CO(2) as a carboxylating agent for the biocatalytic ortho-carboxylation of resorcinol

The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on a large scale. As a proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO(2) (∼30–40 bar) cat...

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Detalles Bibliográficos
Autores principales: Plasch, Katharina, Hofer, Gerhard, Keller, Walter, Hay, Sam, Heyes, Derren J., Dennig, Alexander, Glueck, Silvia M., Faber, Kurt
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5942041/
https://www.ncbi.nlm.nih.gov/pubmed/29780282
http://dx.doi.org/10.1039/c8gc00008e
Descripción
Sumario:The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on a large scale. As a proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO(2) (∼30–40 bar) catalyzed by ortho-benzoic acid decarboxylases with up to 68% conversion. Optimization studies revealed tight pH-control and enzyme stability as the most important determinants.

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