Pressurized CO(2) as a carboxylating agent for the biocatalytic ortho-carboxylation of resorcinol

The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on a large scale. As a proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO(2) (∼30–40 bar) cat...

Descripción completa

Detalles Bibliográficos
Autores principales: Plasch, Katharina, Hofer, Gerhard, Keller, Walter, Hay, Sam, Heyes, Derren J., Dennig, Alexander, Glueck, Silvia M., Faber, Kurt
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5942041/
https://www.ncbi.nlm.nih.gov/pubmed/29780282
http://dx.doi.org/10.1039/c8gc00008e
_version_ 1783321400152424448
author Plasch, Katharina
Hofer, Gerhard
Keller, Walter
Hay, Sam
Heyes, Derren J.
Dennig, Alexander
Glueck, Silvia M.
Faber, Kurt
author_facet Plasch, Katharina
Hofer, Gerhard
Keller, Walter
Hay, Sam
Heyes, Derren J.
Dennig, Alexander
Glueck, Silvia M.
Faber, Kurt
author_sort Plasch, Katharina
collection PubMed
description The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on a large scale. As a proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO(2) (∼30–40 bar) catalyzed by ortho-benzoic acid decarboxylases with up to 68% conversion. Optimization studies revealed tight pH-control and enzyme stability as the most important determinants.
format Online
Article
Text
id pubmed-5942041
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-59420412018-05-18 Pressurized CO(2) as a carboxylating agent for the biocatalytic ortho-carboxylation of resorcinol Plasch, Katharina Hofer, Gerhard Keller, Walter Hay, Sam Heyes, Derren J. Dennig, Alexander Glueck, Silvia M. Faber, Kurt Green Chem Chemistry The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on a large scale. As a proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO(2) (∼30–40 bar) catalyzed by ortho-benzoic acid decarboxylases with up to 68% conversion. Optimization studies revealed tight pH-control and enzyme stability as the most important determinants. Royal Society of Chemistry 2018-04-21 2018-04-03 /pmc/articles/PMC5942041/ /pubmed/29780282 http://dx.doi.org/10.1039/c8gc00008e Text en This journal is © The Royal Society of Chemistry 2018 http://creativecommons.org/licenses/by/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0)
spellingShingle Chemistry
Plasch, Katharina
Hofer, Gerhard
Keller, Walter
Hay, Sam
Heyes, Derren J.
Dennig, Alexander
Glueck, Silvia M.
Faber, Kurt
Pressurized CO(2) as a carboxylating agent for the biocatalytic ortho-carboxylation of resorcinol
title Pressurized CO(2) as a carboxylating agent for the biocatalytic ortho-carboxylation of resorcinol
title_full Pressurized CO(2) as a carboxylating agent for the biocatalytic ortho-carboxylation of resorcinol
title_fullStr Pressurized CO(2) as a carboxylating agent for the biocatalytic ortho-carboxylation of resorcinol
title_full_unstemmed Pressurized CO(2) as a carboxylating agent for the biocatalytic ortho-carboxylation of resorcinol
title_short Pressurized CO(2) as a carboxylating agent for the biocatalytic ortho-carboxylation of resorcinol
title_sort pressurized co(2) as a carboxylating agent for the biocatalytic ortho-carboxylation of resorcinol
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5942041/
https://www.ncbi.nlm.nih.gov/pubmed/29780282
http://dx.doi.org/10.1039/c8gc00008e
work_keys_str_mv AT plaschkatharina pressurizedco2asacarboxylatingagentforthebiocatalyticorthocarboxylationofresorcinol
AT hofergerhard pressurizedco2asacarboxylatingagentforthebiocatalyticorthocarboxylationofresorcinol
AT kellerwalter pressurizedco2asacarboxylatingagentforthebiocatalyticorthocarboxylationofresorcinol
AT haysam pressurizedco2asacarboxylatingagentforthebiocatalyticorthocarboxylationofresorcinol
AT heyesderrenj pressurizedco2asacarboxylatingagentforthebiocatalyticorthocarboxylationofresorcinol
AT dennigalexander pressurizedco2asacarboxylatingagentforthebiocatalyticorthocarboxylationofresorcinol
AT gluecksilviam pressurizedco2asacarboxylatingagentforthebiocatalyticorthocarboxylationofresorcinol
AT faberkurt pressurizedco2asacarboxylatingagentforthebiocatalyticorthocarboxylationofresorcinol

Ejemplares similares