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Bacteriophage Infectivity Against Pseudomonas aeruginosa in Saline Conditions
Pseudomonas aeruginosa is a ubiquitous member of marine biofilm, and reduces thiosulfate to produce toxic hydrogen sulfide gas. In this study, lytic bacteriophages were isolated and applied to inhibit the growth of P. aeruginosa in planktonic mode at different temperature, pH, and salinity. Bacterio...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5942161/ https://www.ncbi.nlm.nih.gov/pubmed/29770130 http://dx.doi.org/10.3389/fmicb.2018.00875 |
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author | Scarascia, Giantommaso Yap, Scott A. Kaksonen, Anna H. Hong, Pei-Ying |
author_facet | Scarascia, Giantommaso Yap, Scott A. Kaksonen, Anna H. Hong, Pei-Ying |
author_sort | Scarascia, Giantommaso |
collection | PubMed |
description | Pseudomonas aeruginosa is a ubiquitous member of marine biofilm, and reduces thiosulfate to produce toxic hydrogen sulfide gas. In this study, lytic bacteriophages were isolated and applied to inhibit the growth of P. aeruginosa in planktonic mode at different temperature, pH, and salinity. Bacteriophages showed optimal infectivity at a multiplicity of infection of 10 in saline conditions, and demonstrated lytic abilities over all tested temperature (25, 30, 37, and 45°C) and pH 6–9. Planktonic P. aeruginosa exhibited significantly longer lag phase and lower specific growth rates upon exposure to bacteriophages. Bacteriophages were subsequently applied to P. aeruginosa-enriched biofilm and were determined to lower the relative abundance of Pseudomonas-related taxa from 0.17 to 5.58% in controls to 0.01–0.61% in treated microbial communities. The relative abundance of Alphaproteobacteria, Pseudoalteromonas, and Planococcaceae decreased, possibly due to the phage-induced disruption of the biofilm matrix. Lastly, when applied to mitigate biofouling of ultrafiltration membranes, bacteriophages were determined to reduce the transmembrane pressure increase by 18% when utilized alone, and by 49% when used in combination with citric acid. The combined treatment was more effective compared with the citric acid treatment alone, which reported ca. 30% transmembrane pressure reduction. Collectively, the findings demonstrated that bacteriophages can be used as a biocidal agent to mitigate undesirable P. aeruginosa-associated problems in seawater applications. |
format | Online Article Text |
id | pubmed-5942161 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-59421612018-05-16 Bacteriophage Infectivity Against Pseudomonas aeruginosa in Saline Conditions Scarascia, Giantommaso Yap, Scott A. Kaksonen, Anna H. Hong, Pei-Ying Front Microbiol Microbiology Pseudomonas aeruginosa is a ubiquitous member of marine biofilm, and reduces thiosulfate to produce toxic hydrogen sulfide gas. In this study, lytic bacteriophages were isolated and applied to inhibit the growth of P. aeruginosa in planktonic mode at different temperature, pH, and salinity. Bacteriophages showed optimal infectivity at a multiplicity of infection of 10 in saline conditions, and demonstrated lytic abilities over all tested temperature (25, 30, 37, and 45°C) and pH 6–9. Planktonic P. aeruginosa exhibited significantly longer lag phase and lower specific growth rates upon exposure to bacteriophages. Bacteriophages were subsequently applied to P. aeruginosa-enriched biofilm and were determined to lower the relative abundance of Pseudomonas-related taxa from 0.17 to 5.58% in controls to 0.01–0.61% in treated microbial communities. The relative abundance of Alphaproteobacteria, Pseudoalteromonas, and Planococcaceae decreased, possibly due to the phage-induced disruption of the biofilm matrix. Lastly, when applied to mitigate biofouling of ultrafiltration membranes, bacteriophages were determined to reduce the transmembrane pressure increase by 18% when utilized alone, and by 49% when used in combination with citric acid. The combined treatment was more effective compared with the citric acid treatment alone, which reported ca. 30% transmembrane pressure reduction. Collectively, the findings demonstrated that bacteriophages can be used as a biocidal agent to mitigate undesirable P. aeruginosa-associated problems in seawater applications. Frontiers Media S.A. 2018-05-02 /pmc/articles/PMC5942161/ /pubmed/29770130 http://dx.doi.org/10.3389/fmicb.2018.00875 Text en Copyright © 2018 Scarascia, Yap, Kaksonen and Hong. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Scarascia, Giantommaso Yap, Scott A. Kaksonen, Anna H. Hong, Pei-Ying Bacteriophage Infectivity Against Pseudomonas aeruginosa in Saline Conditions |
title | Bacteriophage Infectivity Against Pseudomonas aeruginosa in Saline Conditions |
title_full | Bacteriophage Infectivity Against Pseudomonas aeruginosa in Saline Conditions |
title_fullStr | Bacteriophage Infectivity Against Pseudomonas aeruginosa in Saline Conditions |
title_full_unstemmed | Bacteriophage Infectivity Against Pseudomonas aeruginosa in Saline Conditions |
title_short | Bacteriophage Infectivity Against Pseudomonas aeruginosa in Saline Conditions |
title_sort | bacteriophage infectivity against pseudomonas aeruginosa in saline conditions |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5942161/ https://www.ncbi.nlm.nih.gov/pubmed/29770130 http://dx.doi.org/10.3389/fmicb.2018.00875 |
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