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Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents

Genetically-modified animal models have significantly increased our understanding of the complex central nervous system circuits. Among these models, inducible transgenic mice whose specific gene expression can be modulated through a Cre recombinase/LoxP system are useful to study the role of specif...

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Autores principales: Abdallah, Khaled, Nadeau, Francis, Bergeron, Francis, Blouin, Sylvie, Blais, Véronique, Bradbury, Kelly M., Lavoie, Christine L., Parent, Jean-Luc, Gendron, Louis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5943452/
https://www.ncbi.nlm.nih.gov/pubmed/29743652
http://dx.doi.org/10.1038/s41598-018-25626-y
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author Abdallah, Khaled
Nadeau, Francis
Bergeron, Francis
Blouin, Sylvie
Blais, Véronique
Bradbury, Kelly M.
Lavoie, Christine L.
Parent, Jean-Luc
Gendron, Louis
author_facet Abdallah, Khaled
Nadeau, Francis
Bergeron, Francis
Blouin, Sylvie
Blais, Véronique
Bradbury, Kelly M.
Lavoie, Christine L.
Parent, Jean-Luc
Gendron, Louis
author_sort Abdallah, Khaled
collection PubMed
description Genetically-modified animal models have significantly increased our understanding of the complex central nervous system circuits. Among these models, inducible transgenic mice whose specific gene expression can be modulated through a Cre recombinase/LoxP system are useful to study the role of specific peptides and proteins in a given population of cells. In the present study, we describe an efficient approach to selectively deliver a Cre-GFP to dorsal root ganglia (DRG) neurons. First, mice of different ages were injected in both hindpaws with a recombinant adeno-associated virus (rAAV2/9-CBA-Cre-GFP). Using this route of injection in mice at 5 days of age, we report that approximately 20% of all DRG neurons express GFP, 6 to 8 weeks after the infection. The level of infection was reduced by 50% when the virus was administered at 2 weeks of age. Additionally, the virus-mediated delivery of the Cre-GFP was also investigated via the intrathecal route. When injected intrathecally, the rAAV2/9-CBA-Cre-GFP virus infected a much higher proportion of DRG neurons than the intraplantar injection, with up to 51.6% of infected lumbar DRG neurons. Noteworthy, both routes of injection predominantly transduced DRG neurons over spinal and brain neurons.
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spelling pubmed-59434522018-05-14 Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents Abdallah, Khaled Nadeau, Francis Bergeron, Francis Blouin, Sylvie Blais, Véronique Bradbury, Kelly M. Lavoie, Christine L. Parent, Jean-Luc Gendron, Louis Sci Rep Article Genetically-modified animal models have significantly increased our understanding of the complex central nervous system circuits. Among these models, inducible transgenic mice whose specific gene expression can be modulated through a Cre recombinase/LoxP system are useful to study the role of specific peptides and proteins in a given population of cells. In the present study, we describe an efficient approach to selectively deliver a Cre-GFP to dorsal root ganglia (DRG) neurons. First, mice of different ages were injected in both hindpaws with a recombinant adeno-associated virus (rAAV2/9-CBA-Cre-GFP). Using this route of injection in mice at 5 days of age, we report that approximately 20% of all DRG neurons express GFP, 6 to 8 weeks after the infection. The level of infection was reduced by 50% when the virus was administered at 2 weeks of age. Additionally, the virus-mediated delivery of the Cre-GFP was also investigated via the intrathecal route. When injected intrathecally, the rAAV2/9-CBA-Cre-GFP virus infected a much higher proportion of DRG neurons than the intraplantar injection, with up to 51.6% of infected lumbar DRG neurons. Noteworthy, both routes of injection predominantly transduced DRG neurons over spinal and brain neurons. Nature Publishing Group UK 2018-05-09 /pmc/articles/PMC5943452/ /pubmed/29743652 http://dx.doi.org/10.1038/s41598-018-25626-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Abdallah, Khaled
Nadeau, Francis
Bergeron, Francis
Blouin, Sylvie
Blais, Véronique
Bradbury, Kelly M.
Lavoie, Christine L.
Parent, Jean-Luc
Gendron, Louis
Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents
title Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents
title_full Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents
title_fullStr Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents
title_full_unstemmed Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents
title_short Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents
title_sort adeno-associated virus 2/9 delivery of cre recombinase in mouse primary afferents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5943452/
https://www.ncbi.nlm.nih.gov/pubmed/29743652
http://dx.doi.org/10.1038/s41598-018-25626-y
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