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Serum exosomal microRNAs combined with alpha‐fetoprotein as diagnostic markers of hepatocellular carcinoma
Exosomal microRNAs have recently been studied as the potential diagnostic marker for various malignancies, including hepatocellular carcinoma (HCC). The aim of this study was to investigate serum exosomal microRNA profiles as HCC diagnostic marker. Transmission electron microscopy and Western blot w...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5943469/ https://www.ncbi.nlm.nih.gov/pubmed/29573235 http://dx.doi.org/10.1002/cam4.1390 |
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author | Wang, Yurong Zhang, Chunyan Zhang, Pengjun Guo, Guanghong Jiang, Tao Zhao, Xiumei Jiang, Jingjing Huang, Xueliang Tong, Hongli Tian, Yaping |
author_facet | Wang, Yurong Zhang, Chunyan Zhang, Pengjun Guo, Guanghong Jiang, Tao Zhao, Xiumei Jiang, Jingjing Huang, Xueliang Tong, Hongli Tian, Yaping |
author_sort | Wang, Yurong |
collection | PubMed |
description | Exosomal microRNAs have recently been studied as the potential diagnostic marker for various malignancies, including hepatocellular carcinoma (HCC). The aim of this study was to investigate serum exosomal microRNA profiles as HCC diagnostic marker. Transmission electron microscopy and Western blot were used to identify serum exosomes. Deep sequencing was performed to screen differentially expressed microRNAs between HCC (n = 5) and liver cirrhosis (LC, n = 5) groups. Three upregulated and two downregulated microRNAs were selected for qPCR analysis. The levels of selected microRNAs were normalized to Caenorhabditis elegans miR‐39 microRNA mimics. Serum exosomal level of miR‐122, miR‐148a, and miR‐1246 was further analyzed and significantly higher in HCC than LC and normal control (NC) groups (P < 0.001), but not different from chronic hepatitis group (P > 0.05). The receiver operating characteristic curve was used to evaluate the diagnostic performance of candidate microRNAs. Area under the curve (AUC) of miR‐148a was 0.891 [95% confidence interval (CI), 0.809–0.947] in discriminating HCC from LC, remarkably higher than alpha‐fetoprotein (AFP) (AUC: 0.712, 95% CI: 0.607–0.803). Binary logistic regression was adopted to establish the diagnostic model for discriminating HCC from LC. And the combination of miR‐122, miR‐148a, and AFP increased the AUC to 0.931 (95% CI, 0.857–0.973), which can also be applied for distinguishing early HCC from LC. miR‐122 was the best for differentiating HCC from NC (AUC: 0.990, 95% CI, 0.945–1.000). These data suggest that serum exosomal microRNAs signature or their combination with traditional biomarker may be used as a suitable peripheral screening tool for HCC. |
format | Online Article Text |
id | pubmed-5943469 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-59434692018-05-14 Serum exosomal microRNAs combined with alpha‐fetoprotein as diagnostic markers of hepatocellular carcinoma Wang, Yurong Zhang, Chunyan Zhang, Pengjun Guo, Guanghong Jiang, Tao Zhao, Xiumei Jiang, Jingjing Huang, Xueliang Tong, Hongli Tian, Yaping Cancer Med Clinical Cancer Research Exosomal microRNAs have recently been studied as the potential diagnostic marker for various malignancies, including hepatocellular carcinoma (HCC). The aim of this study was to investigate serum exosomal microRNA profiles as HCC diagnostic marker. Transmission electron microscopy and Western blot were used to identify serum exosomes. Deep sequencing was performed to screen differentially expressed microRNAs between HCC (n = 5) and liver cirrhosis (LC, n = 5) groups. Three upregulated and two downregulated microRNAs were selected for qPCR analysis. The levels of selected microRNAs were normalized to Caenorhabditis elegans miR‐39 microRNA mimics. Serum exosomal level of miR‐122, miR‐148a, and miR‐1246 was further analyzed and significantly higher in HCC than LC and normal control (NC) groups (P < 0.001), but not different from chronic hepatitis group (P > 0.05). The receiver operating characteristic curve was used to evaluate the diagnostic performance of candidate microRNAs. Area under the curve (AUC) of miR‐148a was 0.891 [95% confidence interval (CI), 0.809–0.947] in discriminating HCC from LC, remarkably higher than alpha‐fetoprotein (AFP) (AUC: 0.712, 95% CI: 0.607–0.803). Binary logistic regression was adopted to establish the diagnostic model for discriminating HCC from LC. And the combination of miR‐122, miR‐148a, and AFP increased the AUC to 0.931 (95% CI, 0.857–0.973), which can also be applied for distinguishing early HCC from LC. miR‐122 was the best for differentiating HCC from NC (AUC: 0.990, 95% CI, 0.945–1.000). These data suggest that serum exosomal microRNAs signature or their combination with traditional biomarker may be used as a suitable peripheral screening tool for HCC. John Wiley and Sons Inc. 2018-03-23 /pmc/articles/PMC5943469/ /pubmed/29573235 http://dx.doi.org/10.1002/cam4.1390 Text en © 2018 The Authors. Cancer Medicine published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Clinical Cancer Research Wang, Yurong Zhang, Chunyan Zhang, Pengjun Guo, Guanghong Jiang, Tao Zhao, Xiumei Jiang, Jingjing Huang, Xueliang Tong, Hongli Tian, Yaping Serum exosomal microRNAs combined with alpha‐fetoprotein as diagnostic markers of hepatocellular carcinoma |
title | Serum exosomal microRNAs combined with alpha‐fetoprotein as diagnostic markers of hepatocellular carcinoma |
title_full | Serum exosomal microRNAs combined with alpha‐fetoprotein as diagnostic markers of hepatocellular carcinoma |
title_fullStr | Serum exosomal microRNAs combined with alpha‐fetoprotein as diagnostic markers of hepatocellular carcinoma |
title_full_unstemmed | Serum exosomal microRNAs combined with alpha‐fetoprotein as diagnostic markers of hepatocellular carcinoma |
title_short | Serum exosomal microRNAs combined with alpha‐fetoprotein as diagnostic markers of hepatocellular carcinoma |
title_sort | serum exosomal micrornas combined with alpha‐fetoprotein as diagnostic markers of hepatocellular carcinoma |
topic | Clinical Cancer Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5943469/ https://www.ncbi.nlm.nih.gov/pubmed/29573235 http://dx.doi.org/10.1002/cam4.1390 |
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