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A novel rapid quantitative method reveals stathmin‐1 as a promising marker for esophageal squamous cell carcinoma

Stathmin‐1 is a microtubule depolymerization protein that regulates cell division, growth, migration, and invasion. Overexpression of stathmin‐1 has been observed to be associated with metastasis, poor prognosis, and chemoresistance in various human cancers. Our previous studies found that serum sta...

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Autores principales: Yan, Lu, Dong, Xiu, Gao, Jiajia, Liu, Fang, Zhou, Lanping, Sun, Yulin, Zhao, Xiaohang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5943482/
https://www.ncbi.nlm.nih.gov/pubmed/29577639
http://dx.doi.org/10.1002/cam4.1449
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author Yan, Lu
Dong, Xiu
Gao, Jiajia
Liu, Fang
Zhou, Lanping
Sun, Yulin
Zhao, Xiaohang
author_facet Yan, Lu
Dong, Xiu
Gao, Jiajia
Liu, Fang
Zhou, Lanping
Sun, Yulin
Zhao, Xiaohang
author_sort Yan, Lu
collection PubMed
description Stathmin‐1 is a microtubule depolymerization protein that regulates cell division, growth, migration, and invasion. Overexpression of stathmin‐1 has been observed to be associated with metastasis, poor prognosis, and chemoresistance in various human cancers. Our previous studies found that serum stathmin‐1 was significantly elevated in patients with esophageal squamous cell carcinoma (ESCC) by ELISAs. Here, we constructed high‐affinity monoclonal antibodies and then developed a competitive AlphaLISA for rapid, accurate quantitation of stathmin‐1 in serum. Compared to ELISA, our homogeneous AlphaLISA showed better sensitivity and accuracy, a lower limit of detection, and a wider linear range. The measurements of nearly 1000 clinical samples showed that serum stathmin‐1 level increased dramatically in patients with squamous cell carcinoma (SCC), especially in ESCC, with a sensitivity and a specificity of 81% and 94%, respectively. Even for early stage ESCC, stathmin‐1 achieved an area under the receiver operating characteristic curve (AUC) of 0.88. Meanwhile, raised concentrations of stathmin‐1 were associated with lymph node metastasis and advanced cancer stage. Notably, various types of SCC showed significantly higher AUCs in serum stathmin‐1 detection compared to adenocarcinoma. Furthermore, we confirmed that stathmin‐1 was enriched in the oncogenic exosomes, which can explain the reason why it enters into the blood to serve as a tumor surrogate. In conclusion, this large‐scale and systematic study of serum stathmin‐1 measured by our newly established AlphaLISA showed that stathmin‐1 is a very promising diagnostic and predictive marker for SCC in the clinic, especially for ESCC.
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spelling pubmed-59434822018-05-14 A novel rapid quantitative method reveals stathmin‐1 as a promising marker for esophageal squamous cell carcinoma Yan, Lu Dong, Xiu Gao, Jiajia Liu, Fang Zhou, Lanping Sun, Yulin Zhao, Xiaohang Cancer Med Clinical Cancer Research Stathmin‐1 is a microtubule depolymerization protein that regulates cell division, growth, migration, and invasion. Overexpression of stathmin‐1 has been observed to be associated with metastasis, poor prognosis, and chemoresistance in various human cancers. Our previous studies found that serum stathmin‐1 was significantly elevated in patients with esophageal squamous cell carcinoma (ESCC) by ELISAs. Here, we constructed high‐affinity monoclonal antibodies and then developed a competitive AlphaLISA for rapid, accurate quantitation of stathmin‐1 in serum. Compared to ELISA, our homogeneous AlphaLISA showed better sensitivity and accuracy, a lower limit of detection, and a wider linear range. The measurements of nearly 1000 clinical samples showed that serum stathmin‐1 level increased dramatically in patients with squamous cell carcinoma (SCC), especially in ESCC, with a sensitivity and a specificity of 81% and 94%, respectively. Even for early stage ESCC, stathmin‐1 achieved an area under the receiver operating characteristic curve (AUC) of 0.88. Meanwhile, raised concentrations of stathmin‐1 were associated with lymph node metastasis and advanced cancer stage. Notably, various types of SCC showed significantly higher AUCs in serum stathmin‐1 detection compared to adenocarcinoma. Furthermore, we confirmed that stathmin‐1 was enriched in the oncogenic exosomes, which can explain the reason why it enters into the blood to serve as a tumor surrogate. In conclusion, this large‐scale and systematic study of serum stathmin‐1 measured by our newly established AlphaLISA showed that stathmin‐1 is a very promising diagnostic and predictive marker for SCC in the clinic, especially for ESCC. John Wiley and Sons Inc. 2018-03-25 /pmc/articles/PMC5943482/ /pubmed/29577639 http://dx.doi.org/10.1002/cam4.1449 Text en © 2018 The Authors. Cancer Medicine published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Clinical Cancer Research
Yan, Lu
Dong, Xiu
Gao, Jiajia
Liu, Fang
Zhou, Lanping
Sun, Yulin
Zhao, Xiaohang
A novel rapid quantitative method reveals stathmin‐1 as a promising marker for esophageal squamous cell carcinoma
title A novel rapid quantitative method reveals stathmin‐1 as a promising marker for esophageal squamous cell carcinoma
title_full A novel rapid quantitative method reveals stathmin‐1 as a promising marker for esophageal squamous cell carcinoma
title_fullStr A novel rapid quantitative method reveals stathmin‐1 as a promising marker for esophageal squamous cell carcinoma
title_full_unstemmed A novel rapid quantitative method reveals stathmin‐1 as a promising marker for esophageal squamous cell carcinoma
title_short A novel rapid quantitative method reveals stathmin‐1 as a promising marker for esophageal squamous cell carcinoma
title_sort novel rapid quantitative method reveals stathmin‐1 as a promising marker for esophageal squamous cell carcinoma
topic Clinical Cancer Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5943482/
https://www.ncbi.nlm.nih.gov/pubmed/29577639
http://dx.doi.org/10.1002/cam4.1449
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