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Targeted genomic landscape of metastases compared to primary tumours in clear cell metastatic renal cell carcinoma
BACKGROUND: The genomic landscape of primary clear cell renal cell carcinoma (ccRCC) has been well described. However, little is known about cohort genomic alterations (GA) landscape in ccRCC metastases, or how it compares to primary tumours in aggregate. The genomic landscape of metastases may have...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5943584/ https://www.ncbi.nlm.nih.gov/pubmed/29674707 http://dx.doi.org/10.1038/s41416-018-0064-3 |
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author | de Velasco, Guillermo Wankowicz, Stephanie A. Madison, Russell Ali, Siraj M. Norton, Craig Duquette, Audrey Ross, Jeffrey S. Bossé, Dominick Lalani, Aly-Khan A. Miller, Vincent A. Stephens, Philip J. Young, Lauren Hakimi, A. Ari Signoretti, Sabina Pal, Sumanta K. Choueiri, Toni K. |
author_facet | de Velasco, Guillermo Wankowicz, Stephanie A. Madison, Russell Ali, Siraj M. Norton, Craig Duquette, Audrey Ross, Jeffrey S. Bossé, Dominick Lalani, Aly-Khan A. Miller, Vincent A. Stephens, Philip J. Young, Lauren Hakimi, A. Ari Signoretti, Sabina Pal, Sumanta K. Choueiri, Toni K. |
author_sort | de Velasco, Guillermo |
collection | PubMed |
description | BACKGROUND: The genomic landscape of primary clear cell renal cell carcinoma (ccRCC) has been well described. However, little is known about cohort genomic alterations (GA) landscape in ccRCC metastases, or how it compares to primary tumours in aggregate. The genomic landscape of metastases may have biological, clinical, and therapeutic implications. METHODS: We collected targeted next-generation sequencing mutation calls from two independent cohorts and described the metastases GA landscape and descriptively compared it to the GA landscape in primary tumours. RESULTS: The cohort 1 (n = 578) consisted of 349 primary tumours and 229 metastases. Overall, the most common mutations in the metastases were VHL (66.8%), PBRM1 (41.87%), and SETD2 (24.7%). TP53 was more frequently mutated in metastases compared to primary tumours (14.85% versus 8.9%; p = 0.031). No other gene had significant difference in the cohort frequency of mutations between the metastases and primary tumours. Mutation burden was not significantly different between the metastases and primary tumours or between metastatic sites. The second cohort (n = 257) consisted of 177 primary tumours and 80 metastases. No differences in frequency of mutations or mutational burden were observed between primaries and metastases. CONCLUSIONS: These data support the theory that ccRCC primary tumours and metastases encompass a uniform distribution of common genomic alterations tested by next-generation sequencing targeted panels. This study does not address variability between matched primary tumours and metastases or the change in genomic alterations over time and after sequential systemic therapies. |
format | Online Article Text |
id | pubmed-5943584 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59435842019-04-23 Targeted genomic landscape of metastases compared to primary tumours in clear cell metastatic renal cell carcinoma de Velasco, Guillermo Wankowicz, Stephanie A. Madison, Russell Ali, Siraj M. Norton, Craig Duquette, Audrey Ross, Jeffrey S. Bossé, Dominick Lalani, Aly-Khan A. Miller, Vincent A. Stephens, Philip J. Young, Lauren Hakimi, A. Ari Signoretti, Sabina Pal, Sumanta K. Choueiri, Toni K. Br J Cancer Article BACKGROUND: The genomic landscape of primary clear cell renal cell carcinoma (ccRCC) has been well described. However, little is known about cohort genomic alterations (GA) landscape in ccRCC metastases, or how it compares to primary tumours in aggregate. The genomic landscape of metastases may have biological, clinical, and therapeutic implications. METHODS: We collected targeted next-generation sequencing mutation calls from two independent cohorts and described the metastases GA landscape and descriptively compared it to the GA landscape in primary tumours. RESULTS: The cohort 1 (n = 578) consisted of 349 primary tumours and 229 metastases. Overall, the most common mutations in the metastases were VHL (66.8%), PBRM1 (41.87%), and SETD2 (24.7%). TP53 was more frequently mutated in metastases compared to primary tumours (14.85% versus 8.9%; p = 0.031). No other gene had significant difference in the cohort frequency of mutations between the metastases and primary tumours. Mutation burden was not significantly different between the metastases and primary tumours or between metastatic sites. The second cohort (n = 257) consisted of 177 primary tumours and 80 metastases. No differences in frequency of mutations or mutational burden were observed between primaries and metastases. CONCLUSIONS: These data support the theory that ccRCC primary tumours and metastases encompass a uniform distribution of common genomic alterations tested by next-generation sequencing targeted panels. This study does not address variability between matched primary tumours and metastases or the change in genomic alterations over time and after sequential systemic therapies. Nature Publishing Group UK 2018-04-20 2018-05-01 /pmc/articles/PMC5943584/ /pubmed/29674707 http://dx.doi.org/10.1038/s41416-018-0064-3 Text en © Cancer Research UK 2018 https://creativecommons.org/licenses/by/4.0/Note: This work is published under the standard license to publish agreement. After 12 months the work will become freely available and the license terms will switch to a Creative Commons Attribution 4.0 International licence (CC BY 4.0). |
spellingShingle | Article de Velasco, Guillermo Wankowicz, Stephanie A. Madison, Russell Ali, Siraj M. Norton, Craig Duquette, Audrey Ross, Jeffrey S. Bossé, Dominick Lalani, Aly-Khan A. Miller, Vincent A. Stephens, Philip J. Young, Lauren Hakimi, A. Ari Signoretti, Sabina Pal, Sumanta K. Choueiri, Toni K. Targeted genomic landscape of metastases compared to primary tumours in clear cell metastatic renal cell carcinoma |
title | Targeted genomic landscape of metastases compared to primary tumours in clear cell metastatic renal cell carcinoma |
title_full | Targeted genomic landscape of metastases compared to primary tumours in clear cell metastatic renal cell carcinoma |
title_fullStr | Targeted genomic landscape of metastases compared to primary tumours in clear cell metastatic renal cell carcinoma |
title_full_unstemmed | Targeted genomic landscape of metastases compared to primary tumours in clear cell metastatic renal cell carcinoma |
title_short | Targeted genomic landscape of metastases compared to primary tumours in clear cell metastatic renal cell carcinoma |
title_sort | targeted genomic landscape of metastases compared to primary tumours in clear cell metastatic renal cell carcinoma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5943584/ https://www.ncbi.nlm.nih.gov/pubmed/29674707 http://dx.doi.org/10.1038/s41416-018-0064-3 |
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