Cargando…

Functional analysis of cell-free RNA using mid-trimester amniotic fluid supernatant in pregnancy with the fetal growth restriction

The prediction and monitoring of fetal growth restriction (FGR) fetuses has become with the use of ultrasound. However, these tools lack the fundamental evidence for the growth of fetus with FGR excluding pathogenic factors. Amniotic fluid samples were obtained from pregnant women for fetal karyotyp...

Descripción completa

Detalles Bibliográficos
Autores principales: Cho, Hee Young, Cho, Yeonkyung, Shin, Yun-Jeong, Park, Jieun, Shim, Sunghan, Jung, Yongwook, Shim, Sungshin, Cha, Donghyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer Health 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5943846/
https://www.ncbi.nlm.nih.gov/pubmed/29480850
http://dx.doi.org/10.1097/MD.0000000000009572
_version_ 1783321707762679808
author Cho, Hee Young
Cho, Yeonkyung
Shin, Yun-Jeong
Park, Jieun
Shim, Sunghan
Jung, Yongwook
Shim, Sungshin
Cha, Donghyun
author_facet Cho, Hee Young
Cho, Yeonkyung
Shin, Yun-Jeong
Park, Jieun
Shim, Sunghan
Jung, Yongwook
Shim, Sungshin
Cha, Donghyun
author_sort Cho, Hee Young
collection PubMed
description The prediction and monitoring of fetal growth restriction (FGR) fetuses has become with the use of ultrasound. However, these tools lack the fundamental evidence for the growth of fetus with FGR excluding pathogenic factors. Amniotic fluid samples were obtained from pregnant women for fetal karyotyping and genetic diagnosis at 16 to 19 weeks of gestation. For this study, 15 FGR and 9 control samples were selected, and cell-free fetal RNA was isolated from each supernatant of the amniotic fluid for microarray analysis. In this study, 411 genes were differentially expressed between the FGR and control group. Of these genes, 316 genes were up-regulated, while 95 genes were down-regulated. In terms of gene ontology, the up-regulated genes were highly related to metabolic process as well as protein synthesis, while the down-regulated genes were related to receptor activity and biological adhesion. In terms of tissue-specific expression, the up-regulated genes were involved in various organs while down-regulated genes were involved only in the brain. In terms of organ-specific expression, many genes were enriched for B-cell lymphoma, pancreas, eye, placenta, epithelium, skin, and muscle. In the functional significance of gene, low-density lipoprotein receptor-related protein 10 (LRP10) was significantly increased (6-fold) and insulin-like growth factor (IGF-2) was dramatically increased (17-fold) in the FGR cases. The results show that the important brain-related genes are predominantly down-regulated in the intrauterine growth restriction fetuses during the second trimester of pregnancy. This study also suggested possible genes related to fetal development such as B-cell lymphoma, LRP10, and IGF-2. To monitor the fetal development, further study may be needed to elucidate the role of the genes identified.
format Online
Article
Text
id pubmed-5943846
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Wolters Kluwer Health
record_format MEDLINE/PubMed
spelling pubmed-59438462018-05-15 Functional analysis of cell-free RNA using mid-trimester amniotic fluid supernatant in pregnancy with the fetal growth restriction Cho, Hee Young Cho, Yeonkyung Shin, Yun-Jeong Park, Jieun Shim, Sunghan Jung, Yongwook Shim, Sungshin Cha, Donghyun Medicine (Baltimore) Research Article The prediction and monitoring of fetal growth restriction (FGR) fetuses has become with the use of ultrasound. However, these tools lack the fundamental evidence for the growth of fetus with FGR excluding pathogenic factors. Amniotic fluid samples were obtained from pregnant women for fetal karyotyping and genetic diagnosis at 16 to 19 weeks of gestation. For this study, 15 FGR and 9 control samples were selected, and cell-free fetal RNA was isolated from each supernatant of the amniotic fluid for microarray analysis. In this study, 411 genes were differentially expressed between the FGR and control group. Of these genes, 316 genes were up-regulated, while 95 genes were down-regulated. In terms of gene ontology, the up-regulated genes were highly related to metabolic process as well as protein synthesis, while the down-regulated genes were related to receptor activity and biological adhesion. In terms of tissue-specific expression, the up-regulated genes were involved in various organs while down-regulated genes were involved only in the brain. In terms of organ-specific expression, many genes were enriched for B-cell lymphoma, pancreas, eye, placenta, epithelium, skin, and muscle. In the functional significance of gene, low-density lipoprotein receptor-related protein 10 (LRP10) was significantly increased (6-fold) and insulin-like growth factor (IGF-2) was dramatically increased (17-fold) in the FGR cases. The results show that the important brain-related genes are predominantly down-regulated in the intrauterine growth restriction fetuses during the second trimester of pregnancy. This study also suggested possible genes related to fetal development such as B-cell lymphoma, LRP10, and IGF-2. To monitor the fetal development, further study may be needed to elucidate the role of the genes identified. Wolters Kluwer Health 2018-01-12 /pmc/articles/PMC5943846/ /pubmed/29480850 http://dx.doi.org/10.1097/MD.0000000000009572 Text en Copyright © 2018 the Author(s). Published by Wolters Kluwer Health, Inc. http://creativecommons.org/licenses/by-nc-nd/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0
spellingShingle Research Article
Cho, Hee Young
Cho, Yeonkyung
Shin, Yun-Jeong
Park, Jieun
Shim, Sunghan
Jung, Yongwook
Shim, Sungshin
Cha, Donghyun
Functional analysis of cell-free RNA using mid-trimester amniotic fluid supernatant in pregnancy with the fetal growth restriction
title Functional analysis of cell-free RNA using mid-trimester amniotic fluid supernatant in pregnancy with the fetal growth restriction
title_full Functional analysis of cell-free RNA using mid-trimester amniotic fluid supernatant in pregnancy with the fetal growth restriction
title_fullStr Functional analysis of cell-free RNA using mid-trimester amniotic fluid supernatant in pregnancy with the fetal growth restriction
title_full_unstemmed Functional analysis of cell-free RNA using mid-trimester amniotic fluid supernatant in pregnancy with the fetal growth restriction
title_short Functional analysis of cell-free RNA using mid-trimester amniotic fluid supernatant in pregnancy with the fetal growth restriction
title_sort functional analysis of cell-free rna using mid-trimester amniotic fluid supernatant in pregnancy with the fetal growth restriction
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5943846/
https://www.ncbi.nlm.nih.gov/pubmed/29480850
http://dx.doi.org/10.1097/MD.0000000000009572
work_keys_str_mv AT choheeyoung functionalanalysisofcellfreernausingmidtrimesteramnioticfluidsupernatantinpregnancywiththefetalgrowthrestriction
AT choyeonkyung functionalanalysisofcellfreernausingmidtrimesteramnioticfluidsupernatantinpregnancywiththefetalgrowthrestriction
AT shinyunjeong functionalanalysisofcellfreernausingmidtrimesteramnioticfluidsupernatantinpregnancywiththefetalgrowthrestriction
AT parkjieun functionalanalysisofcellfreernausingmidtrimesteramnioticfluidsupernatantinpregnancywiththefetalgrowthrestriction
AT shimsunghan functionalanalysisofcellfreernausingmidtrimesteramnioticfluidsupernatantinpregnancywiththefetalgrowthrestriction
AT jungyongwook functionalanalysisofcellfreernausingmidtrimesteramnioticfluidsupernatantinpregnancywiththefetalgrowthrestriction
AT shimsungshin functionalanalysisofcellfreernausingmidtrimesteramnioticfluidsupernatantinpregnancywiththefetalgrowthrestriction
AT chadonghyun functionalanalysisofcellfreernausingmidtrimesteramnioticfluidsupernatantinpregnancywiththefetalgrowthrestriction