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Transcriptome sequencing and analysis during seed growth and development in Euryale ferox Salisb

BACKGROUND: Euryale ferox Salisb., an annual aquatic plant, is the only species in the genus Euryale in the Nymphaeaceae. Seeds of E. ferox are a nutritious food and also used in traditional Chinese medicine (Qian Shi in Mandarin). The molecular events that occurred during seed development in E. fer...

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Autores principales: Liu, Xian, He, Zhen, Yin, Yulai, Xu, Xu, Wu, Weiwen, Li, Liangjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5944168/
https://www.ncbi.nlm.nih.gov/pubmed/29743016
http://dx.doi.org/10.1186/s12864-018-4707-9
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author Liu, Xian
He, Zhen
Yin, Yulai
Xu, Xu
Wu, Weiwen
Li, Liangjun
author_facet Liu, Xian
He, Zhen
Yin, Yulai
Xu, Xu
Wu, Weiwen
Li, Liangjun
author_sort Liu, Xian
collection PubMed
description BACKGROUND: Euryale ferox Salisb., an annual aquatic plant, is the only species in the genus Euryale in the Nymphaeaceae. Seeds of E. ferox are a nutritious food and also used in traditional Chinese medicine (Qian Shi in Mandarin). The molecular events that occurred during seed development in E. ferox have not yet been characterized. In this study, we performed transcriptomic analysis of four developmental stages (T1, T2, T3, and T4) in E. ferox seeds with three biological replicates per developmental stage to understand the physiological and biochemical processes during E. ferox seeds development. RESULTS: 313,844,425 clean reads were assembled into 160,107 transcripts and 85,006 unigenes with N50 lengths of 2052 bp and 1399 bp, respectively. The unigenes were annotated using five public databases (NR, COG, Swiss-Prot, KEGG, and GO). In the KEGG database, all of the unigenes were assigned to 127 pathways, of which phenylpropanoid biosynthesis was associated with the synthesis of secondary metabolites during E. ferox seed growth and development. Phenylalanine ammonia-lyase (PAL) as the first key enzyme catalyzed the conversion of phenylalanine to trans-cinnamic acid, then was related to the synthesis of flavonoids, lignins and alkaloid. The expression of PAL1 reached its peak at T3 stage, followed by a slight decrease at T4 stage. Cytochrome P450 (P450), encoded by CYP84A1 (which also called ferulate-5-hydroxylase (F5H) in Arabidopsis), was mainly involved in the biosynthesis of lignins. CONCLUSIONS: Our study provides a transcriptomic analysis to better understand the morphological changes and the accumulation of medicinal components during E. ferox seed development. The increasing expression of PAL and P450 encoded genes in phenylpropanoid biosynthesis may promote the maturation of E. ferox seed including size, color, hardness and accumulation of medicinal components. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-4707-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-59441682018-05-14 Transcriptome sequencing and analysis during seed growth and development in Euryale ferox Salisb Liu, Xian He, Zhen Yin, Yulai Xu, Xu Wu, Weiwen Li, Liangjun BMC Genomics Research Article BACKGROUND: Euryale ferox Salisb., an annual aquatic plant, is the only species in the genus Euryale in the Nymphaeaceae. Seeds of E. ferox are a nutritious food and also used in traditional Chinese medicine (Qian Shi in Mandarin). The molecular events that occurred during seed development in E. ferox have not yet been characterized. In this study, we performed transcriptomic analysis of four developmental stages (T1, T2, T3, and T4) in E. ferox seeds with three biological replicates per developmental stage to understand the physiological and biochemical processes during E. ferox seeds development. RESULTS: 313,844,425 clean reads were assembled into 160,107 transcripts and 85,006 unigenes with N50 lengths of 2052 bp and 1399 bp, respectively. The unigenes were annotated using five public databases (NR, COG, Swiss-Prot, KEGG, and GO). In the KEGG database, all of the unigenes were assigned to 127 pathways, of which phenylpropanoid biosynthesis was associated with the synthesis of secondary metabolites during E. ferox seed growth and development. Phenylalanine ammonia-lyase (PAL) as the first key enzyme catalyzed the conversion of phenylalanine to trans-cinnamic acid, then was related to the synthesis of flavonoids, lignins and alkaloid. The expression of PAL1 reached its peak at T3 stage, followed by a slight decrease at T4 stage. Cytochrome P450 (P450), encoded by CYP84A1 (which also called ferulate-5-hydroxylase (F5H) in Arabidopsis), was mainly involved in the biosynthesis of lignins. CONCLUSIONS: Our study provides a transcriptomic analysis to better understand the morphological changes and the accumulation of medicinal components during E. ferox seed development. The increasing expression of PAL and P450 encoded genes in phenylpropanoid biosynthesis may promote the maturation of E. ferox seed including size, color, hardness and accumulation of medicinal components. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-4707-9) contains supplementary material, which is available to authorized users. BioMed Central 2018-05-09 /pmc/articles/PMC5944168/ /pubmed/29743016 http://dx.doi.org/10.1186/s12864-018-4707-9 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Liu, Xian
He, Zhen
Yin, Yulai
Xu, Xu
Wu, Weiwen
Li, Liangjun
Transcriptome sequencing and analysis during seed growth and development in Euryale ferox Salisb
title Transcriptome sequencing and analysis during seed growth and development in Euryale ferox Salisb
title_full Transcriptome sequencing and analysis during seed growth and development in Euryale ferox Salisb
title_fullStr Transcriptome sequencing and analysis during seed growth and development in Euryale ferox Salisb
title_full_unstemmed Transcriptome sequencing and analysis during seed growth and development in Euryale ferox Salisb
title_short Transcriptome sequencing and analysis during seed growth and development in Euryale ferox Salisb
title_sort transcriptome sequencing and analysis during seed growth and development in euryale ferox salisb
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5944168/
https://www.ncbi.nlm.nih.gov/pubmed/29743016
http://dx.doi.org/10.1186/s12864-018-4707-9
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