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Antiulcer Activity and Potential Mechanism of Action of the Leaves of Spondias mombin L.
Spondias mombin L. is used in folk medicine for the treatment of inflammation and gastrointestinal diseases. Our study investigated the antiulcer activity of S. mombin ethanolic extract (SmEE) and its majority compounds gallic acid (GA) and ellagic acid (EA). Phytochemical characterization was perfo...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5944294/ https://www.ncbi.nlm.nih.gov/pubmed/29854075 http://dx.doi.org/10.1155/2018/1731459 |
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author | Brito, Samara Alves de Almeida, Cynthia Layse Ferreira de Santana, Temístocles Italo da Silva Oliveira, Alisson Rodrigo do Nascimento Figueiredo, Jéssica Carla Bezerra Souza, Isis Torres de Almeida, Lécio Leone da Silva, Márcia Vanusa Borges, Augusto Santos de Medeiros, Jonathan Wagner da Costa Silva Neto, Jacinto de Cássia Ribeiro Gonçalves, Rita Kitagawa, Rodrigo Rezende Sant'Ana, Antônio Euzébio Goulart Rolim, Larissa Araújo de Menezes, Irwin Rose Alencar da Silva, Teresinha Gonçalves Caldas, Germana Freire Rocha Wanderley, Almir Gonçalves |
author_facet | Brito, Samara Alves de Almeida, Cynthia Layse Ferreira de Santana, Temístocles Italo da Silva Oliveira, Alisson Rodrigo do Nascimento Figueiredo, Jéssica Carla Bezerra Souza, Isis Torres de Almeida, Lécio Leone da Silva, Márcia Vanusa Borges, Augusto Santos de Medeiros, Jonathan Wagner da Costa Silva Neto, Jacinto de Cássia Ribeiro Gonçalves, Rita Kitagawa, Rodrigo Rezende Sant'Ana, Antônio Euzébio Goulart Rolim, Larissa Araújo de Menezes, Irwin Rose Alencar da Silva, Teresinha Gonçalves Caldas, Germana Freire Rocha Wanderley, Almir Gonçalves |
author_sort | Brito, Samara Alves |
collection | PubMed |
description | Spondias mombin L. is used in folk medicine for the treatment of inflammation and gastrointestinal diseases. Our study investigated the antiulcer activity of S. mombin ethanolic extract (SmEE) and its majority compounds gallic acid (GA) and ellagic acid (EA). Phytochemical characterization was performed by HPLC. The SmEE was screened for in vitro antioxidant activities using phosphomolybdenum, ABTS, DPPH, and FRAP assays. The antiulcer activity of SmEE, GA, EA, or GA + EA was evaluated by gastric lesion models induced by absolute ethanol and indomethacin. Following this, it is capable of stimulating mucus production, antisecretory capacity, and the influence of −SH groups and NO in the effect of SmEE. Its healing activity was demonstrated by acetic acid-induced chronic ulcer model. Anti-Helicobacter pylori activity was assessed by determining the MIC of the SmEE (64–1024 μg/mL). The HPLC results identified the presence of gallic acid and ellagic acid in SmEE. The extract showed antioxidant activity in vitro. SmEE (50, 100, and 200 mg/kg) reduced the area of ulcerative lesions induced by ethanol in 23.8, 90.3, and 90.2%, respectively. In NSAID model, the SmEE induced protection of 36.8, 49.4, and 49.9%, respectively. GA (10 mg/kg) or EA (7 mg/kg) or the association of GA + EA (10 + 7 mg/kg) inhibited the ethanol-induced lesions in 71.8, 70.9, and 94.9%, respectively, indicating synergistic action. SmEE (100 mg/kg) decreased acid secretion and H(+) concentration in the gastric contents, increased levels of mucus, and showed to be dependent of −SH groups and NO on the protection of the gastric mucosa. In chronic ulcer model, SmEE reduced the gastric area lesion. SmEE showed anti-H. pylori activity. In conclusion, our study showed that SmEE has antiulcerogenic activity. GA and EA are isolated gastric protectors and, when associated, acted synergistically to protect the gastric mucosa. |
format | Online Article Text |
id | pubmed-5944294 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-59442942018-05-31 Antiulcer Activity and Potential Mechanism of Action of the Leaves of Spondias mombin L. Brito, Samara Alves de Almeida, Cynthia Layse Ferreira de Santana, Temístocles Italo da Silva Oliveira, Alisson Rodrigo do Nascimento Figueiredo, Jéssica Carla Bezerra Souza, Isis Torres de Almeida, Lécio Leone da Silva, Márcia Vanusa Borges, Augusto Santos de Medeiros, Jonathan Wagner da Costa Silva Neto, Jacinto de Cássia Ribeiro Gonçalves, Rita Kitagawa, Rodrigo Rezende Sant'Ana, Antônio Euzébio Goulart Rolim, Larissa Araújo de Menezes, Irwin Rose Alencar da Silva, Teresinha Gonçalves Caldas, Germana Freire Rocha Wanderley, Almir Gonçalves Oxid Med Cell Longev Research Article Spondias mombin L. is used in folk medicine for the treatment of inflammation and gastrointestinal diseases. Our study investigated the antiulcer activity of S. mombin ethanolic extract (SmEE) and its majority compounds gallic acid (GA) and ellagic acid (EA). Phytochemical characterization was performed by HPLC. The SmEE was screened for in vitro antioxidant activities using phosphomolybdenum, ABTS, DPPH, and FRAP assays. The antiulcer activity of SmEE, GA, EA, or GA + EA was evaluated by gastric lesion models induced by absolute ethanol and indomethacin. Following this, it is capable of stimulating mucus production, antisecretory capacity, and the influence of −SH groups and NO in the effect of SmEE. Its healing activity was demonstrated by acetic acid-induced chronic ulcer model. Anti-Helicobacter pylori activity was assessed by determining the MIC of the SmEE (64–1024 μg/mL). The HPLC results identified the presence of gallic acid and ellagic acid in SmEE. The extract showed antioxidant activity in vitro. SmEE (50, 100, and 200 mg/kg) reduced the area of ulcerative lesions induced by ethanol in 23.8, 90.3, and 90.2%, respectively. In NSAID model, the SmEE induced protection of 36.8, 49.4, and 49.9%, respectively. GA (10 mg/kg) or EA (7 mg/kg) or the association of GA + EA (10 + 7 mg/kg) inhibited the ethanol-induced lesions in 71.8, 70.9, and 94.9%, respectively, indicating synergistic action. SmEE (100 mg/kg) decreased acid secretion and H(+) concentration in the gastric contents, increased levels of mucus, and showed to be dependent of −SH groups and NO on the protection of the gastric mucosa. In chronic ulcer model, SmEE reduced the gastric area lesion. SmEE showed anti-H. pylori activity. In conclusion, our study showed that SmEE has antiulcerogenic activity. GA and EA are isolated gastric protectors and, when associated, acted synergistically to protect the gastric mucosa. Hindawi 2018-04-26 /pmc/articles/PMC5944294/ /pubmed/29854075 http://dx.doi.org/10.1155/2018/1731459 Text en Copyright © 2018 Samara Alves Brito et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Brito, Samara Alves de Almeida, Cynthia Layse Ferreira de Santana, Temístocles Italo da Silva Oliveira, Alisson Rodrigo do Nascimento Figueiredo, Jéssica Carla Bezerra Souza, Isis Torres de Almeida, Lécio Leone da Silva, Márcia Vanusa Borges, Augusto Santos de Medeiros, Jonathan Wagner da Costa Silva Neto, Jacinto de Cássia Ribeiro Gonçalves, Rita Kitagawa, Rodrigo Rezende Sant'Ana, Antônio Euzébio Goulart Rolim, Larissa Araújo de Menezes, Irwin Rose Alencar da Silva, Teresinha Gonçalves Caldas, Germana Freire Rocha Wanderley, Almir Gonçalves Antiulcer Activity and Potential Mechanism of Action of the Leaves of Spondias mombin L. |
title | Antiulcer Activity and Potential Mechanism of Action of the Leaves of Spondias mombin L. |
title_full | Antiulcer Activity and Potential Mechanism of Action of the Leaves of Spondias mombin L. |
title_fullStr | Antiulcer Activity and Potential Mechanism of Action of the Leaves of Spondias mombin L. |
title_full_unstemmed | Antiulcer Activity and Potential Mechanism of Action of the Leaves of Spondias mombin L. |
title_short | Antiulcer Activity and Potential Mechanism of Action of the Leaves of Spondias mombin L. |
title_sort | antiulcer activity and potential mechanism of action of the leaves of spondias mombin l. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5944294/ https://www.ncbi.nlm.nih.gov/pubmed/29854075 http://dx.doi.org/10.1155/2018/1731459 |
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