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Biochemical and Molecular Screening of Varieties of Chili Plants that are Resistant against Fusarium Wilt Infection

Pakistan holds the position of top chilies producers. So Capsicum annuum L. production in Pakistan should be promoted by combating against diseases. The only solution is to cultivate resistant varieties. Presently six chili varieties were treated with Fusarium oxysporum Schlecht. and screened for th...

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Autores principales: Shafique, Sobiya, Shafique, Shazia, Ahmad, Aqeel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Akadémiai Kiadó 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5944421/
https://www.ncbi.nlm.nih.gov/pubmed/29760960
http://dx.doi.org/10.1556/1886.2017.00031
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author Shafique, Sobiya
Shafique, Shazia
Ahmad, Aqeel
author_facet Shafique, Sobiya
Shafique, Shazia
Ahmad, Aqeel
author_sort Shafique, Sobiya
collection PubMed
description Pakistan holds the position of top chilies producers. So Capsicum annuum L. production in Pakistan should be promoted by combating against diseases. The only solution is to cultivate resistant varieties. Presently six chili varieties were treated with Fusarium oxysporum Schlecht. and screened for the most resistant and the most susceptible varieties. Representative varieties were evaluated for their biochemical and transcriptional profiles to discover the bases of antifungal-resistance. Results concluded that the most resistant variety was “Dandicut” and the most susceptible was “Ghotki”. Tannins, coumarins, flavonoids, phenolics, Riboflavins and saponins were observed in higher quantities in Dandicut as compared to Ghotki. Defense related enzymes i.e. polyphenol oxidase, phenyl ammonia lyase and peroxidase were found in elevated amounts in Dandicut than in Ghotki. Transcriptional results showed that defense related genes i.e. PR2a, acidic glucanase; Chitinase 3, acidic; Osmotin-like PR5 and Metallothionein 2b-like had higher expressional rates in Dandicut. Pearson’s correlation coefficient revealed stronger direct interaction in signal transduction and salicylic acid pathway. Resistance of chili varieties is salicylic acid based. Results obtained from this study not only help to improve chili production in Pakistan but also facilitate variety development operations. Moreover, it also constructed a scale to evaluate innate resistance among varieties.
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spelling pubmed-59444212018-05-14 Biochemical and Molecular Screening of Varieties of Chili Plants that are Resistant against Fusarium Wilt Infection Shafique, Sobiya Shafique, Shazia Ahmad, Aqeel Eur J Microbiol Immunol (Bp) Original Research Paper Pakistan holds the position of top chilies producers. So Capsicum annuum L. production in Pakistan should be promoted by combating against diseases. The only solution is to cultivate resistant varieties. Presently six chili varieties were treated with Fusarium oxysporum Schlecht. and screened for the most resistant and the most susceptible varieties. Representative varieties were evaluated for their biochemical and transcriptional profiles to discover the bases of antifungal-resistance. Results concluded that the most resistant variety was “Dandicut” and the most susceptible was “Ghotki”. Tannins, coumarins, flavonoids, phenolics, Riboflavins and saponins were observed in higher quantities in Dandicut as compared to Ghotki. Defense related enzymes i.e. polyphenol oxidase, phenyl ammonia lyase and peroxidase were found in elevated amounts in Dandicut than in Ghotki. Transcriptional results showed that defense related genes i.e. PR2a, acidic glucanase; Chitinase 3, acidic; Osmotin-like PR5 and Metallothionein 2b-like had higher expressional rates in Dandicut. Pearson’s correlation coefficient revealed stronger direct interaction in signal transduction and salicylic acid pathway. Resistance of chili varieties is salicylic acid based. Results obtained from this study not only help to improve chili production in Pakistan but also facilitate variety development operations. Moreover, it also constructed a scale to evaluate innate resistance among varieties. Akadémiai Kiadó 2018-04-11 /pmc/articles/PMC5944421/ /pubmed/29760960 http://dx.doi.org/10.1556/1886.2017.00031 Text en © 2018, The Author(s) http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted use, distribution, and reproduction in any medium for non-commercial purposes, provided the original author and source are credited, a link to the CC License is provided, and changes – if any – are indicated.
spellingShingle Original Research Paper
Shafique, Sobiya
Shafique, Shazia
Ahmad, Aqeel
Biochemical and Molecular Screening of Varieties of Chili Plants that are Resistant against Fusarium Wilt Infection
title Biochemical and Molecular Screening of Varieties of Chili Plants that are Resistant against Fusarium Wilt Infection
title_full Biochemical and Molecular Screening of Varieties of Chili Plants that are Resistant against Fusarium Wilt Infection
title_fullStr Biochemical and Molecular Screening of Varieties of Chili Plants that are Resistant against Fusarium Wilt Infection
title_full_unstemmed Biochemical and Molecular Screening of Varieties of Chili Plants that are Resistant against Fusarium Wilt Infection
title_short Biochemical and Molecular Screening of Varieties of Chili Plants that are Resistant against Fusarium Wilt Infection
title_sort biochemical and molecular screening of varieties of chili plants that are resistant against fusarium wilt infection
topic Original Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5944421/
https://www.ncbi.nlm.nih.gov/pubmed/29760960
http://dx.doi.org/10.1556/1886.2017.00031
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