Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures

Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA t...

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Detalles Bibliográficos
Autores principales: Manuguerra, Ilenia, Croce, Stefano, El-Sagheer, Afaf H., Krissanaprasit, Abhichart, Brown, Tom, Gothelf, Kurt V., Manetto, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5944424/
https://www.ncbi.nlm.nih.gov/pubmed/29662975
http://dx.doi.org/10.1039/c8cc00738a
Descripción
Sumario:Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA that is compatible with polymerase enzymes.

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