Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures

Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA t...

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Detalles Bibliográficos
Autores principales: Manuguerra, Ilenia, Croce, Stefano, El-Sagheer, Afaf H., Krissanaprasit, Abhichart, Brown, Tom, Gothelf, Kurt V., Manetto, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5944424/
https://www.ncbi.nlm.nih.gov/pubmed/29662975
http://dx.doi.org/10.1039/c8cc00738a
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author Manuguerra, Ilenia
Croce, Stefano
El-Sagheer, Afaf H.
Krissanaprasit, Abhichart
Brown, Tom
Gothelf, Kurt V.
Manetto, Antonio
author_facet Manuguerra, Ilenia
Croce, Stefano
El-Sagheer, Afaf H.
Krissanaprasit, Abhichart
Brown, Tom
Gothelf, Kurt V.
Manetto, Antonio
author_sort Manuguerra, Ilenia
collection PubMed
description Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA that is compatible with polymerase enzymes.
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spelling pubmed-59444242018-05-18 Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures Manuguerra, Ilenia Croce, Stefano El-Sagheer, Afaf H. Krissanaprasit, Abhichart Brown, Tom Gothelf, Kurt V. Manetto, Antonio Chem Commun (Camb) Chemistry Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA that is compatible with polymerase enzymes. Royal Society of Chemistry 2018-05-04 2018-04-17 /pmc/articles/PMC5944424/ /pubmed/29662975 http://dx.doi.org/10.1039/c8cc00738a Text en This journal is © The Royal Society of Chemistry 2018 http://creativecommons.org/licenses/by/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0)
spellingShingle Chemistry
Manuguerra, Ilenia
Croce, Stefano
El-Sagheer, Afaf H.
Krissanaprasit, Abhichart
Brown, Tom
Gothelf, Kurt V.
Manetto, Antonio
Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures
title Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures
title_full Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures
title_fullStr Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures
title_full_unstemmed Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures
title_short Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures
title_sort gene assembly via one-pot chemical ligation of dna promoted by dna nanostructures
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5944424/
https://www.ncbi.nlm.nih.gov/pubmed/29662975
http://dx.doi.org/10.1039/c8cc00738a
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