Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols

Two ‘turn on’ TCF-based fluorescence probes were developed for the detection of biological thiols (TCF-GSH and TCFCl-GSH). TCF-GSH was shown to have a high sensitivity towards glutathione (GSH) with a 0.28 μM limit of detection. Unfortunately, at higher GSH concentrations the fluorescence intensity...

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Autores principales: Sedgwick, Adam C., Gardiner, Jordan E., Kim, Gyoungmi, Yevglevskis, Maksims, Lloyd, Matthew D., Jenkins, A. Toby A., Bull, Steven D., Yoon, Juyoung, James, Tony D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5944426/
https://www.ncbi.nlm.nih.gov/pubmed/29683468
http://dx.doi.org/10.1039/c8cc01661e
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author Sedgwick, Adam C.
Gardiner, Jordan E.
Kim, Gyoungmi
Yevglevskis, Maksims
Lloyd, Matthew D.
Jenkins, A. Toby A.
Bull, Steven D.
Yoon, Juyoung
James, Tony D.
author_facet Sedgwick, Adam C.
Gardiner, Jordan E.
Kim, Gyoungmi
Yevglevskis, Maksims
Lloyd, Matthew D.
Jenkins, A. Toby A.
Bull, Steven D.
Yoon, Juyoung
James, Tony D.
author_sort Sedgwick, Adam C.
collection PubMed
description Two ‘turn on’ TCF-based fluorescence probes were developed for the detection of biological thiols (TCF-GSH and TCFCl-GSH). TCF-GSH was shown to have a high sensitivity towards glutathione (GSH) with a 0.28 μM limit of detection. Unfortunately, at higher GSH concentrations the fluorescence intensity of TCF-GSH decreased and toxicity was observed for TCF-GSH in live cells. However, TCFCl-GSH was shown to be able to detect GSH at biologically relevant concentrations with a 0.45 μM limit of detection. No toxicity was found for TCFCl-GSH and a clear ‘turn on’ with good photostability was observed for the exogenous addition of GSH, Cys and HCys. Furthermore, TCFCl-GSH was used to evaluate the effects of drug treatment on the levels of GSH in live cells.
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spelling pubmed-59444262018-05-18 Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols Sedgwick, Adam C. Gardiner, Jordan E. Kim, Gyoungmi Yevglevskis, Maksims Lloyd, Matthew D. Jenkins, A. Toby A. Bull, Steven D. Yoon, Juyoung James, Tony D. Chem Commun (Camb) Chemistry Two ‘turn on’ TCF-based fluorescence probes were developed for the detection of biological thiols (TCF-GSH and TCFCl-GSH). TCF-GSH was shown to have a high sensitivity towards glutathione (GSH) with a 0.28 μM limit of detection. Unfortunately, at higher GSH concentrations the fluorescence intensity of TCF-GSH decreased and toxicity was observed for TCF-GSH in live cells. However, TCFCl-GSH was shown to be able to detect GSH at biologically relevant concentrations with a 0.45 μM limit of detection. No toxicity was found for TCFCl-GSH and a clear ‘turn on’ with good photostability was observed for the exogenous addition of GSH, Cys and HCys. Furthermore, TCFCl-GSH was used to evaluate the effects of drug treatment on the levels of GSH in live cells. Royal Society of Chemistry 2018-05-11 2018-04-17 /pmc/articles/PMC5944426/ /pubmed/29683468 http://dx.doi.org/10.1039/c8cc01661e Text en This journal is © The Royal Society of Chemistry 2018 http://creativecommons.org/licenses/by/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0)
spellingShingle Chemistry
Sedgwick, Adam C.
Gardiner, Jordan E.
Kim, Gyoungmi
Yevglevskis, Maksims
Lloyd, Matthew D.
Jenkins, A. Toby A.
Bull, Steven D.
Yoon, Juyoung
James, Tony D.
Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols
title Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols
title_full Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols
title_fullStr Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols
title_full_unstemmed Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols
title_short Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols
title_sort long-wavelength tcf-based fluorescence probes for the detection and intracellular imaging of biological thiols
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5944426/
https://www.ncbi.nlm.nih.gov/pubmed/29683468
http://dx.doi.org/10.1039/c8cc01661e
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