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Increased level and fragmentation of plasma circulating cell-free DNA are diagnostic and prognostic markers for renal cell carcinoma

BACKGROUND: Reliable biomarkers for renal cell carcinoma (RCC) have yet to be found. Circulating cell-free DNA (cfDNA) is an emerging resource for the diagnosis and prognosis of various cancers. This study aims to identify novel blood biomarkers for RCC. MATERIALS AND METHODS: Plasma cfDNA was extra...

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Autores principales: Yamamoto, Yoshiyuki, Uemura, Motohide, Nakano, Kosuke, Hayashi, Yujiro, Wang, Cong, Ishizuya, Yu, Kinouchi, Toshiro, Hayashi, Takuji, Matsuzaki, Kyosuke, Jingushi, Kentaro, Kato, Taigo, Kawashima, Atsunari, Ujike, Takeshi, Nagahara, Akira, Fujita, Kazutoshi, Imamura, Ryoichi, Nonomura, Norio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5945531/
https://www.ncbi.nlm.nih.gov/pubmed/29755665
http://dx.doi.org/10.18632/oncotarget.24943
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author Yamamoto, Yoshiyuki
Uemura, Motohide
Nakano, Kosuke
Hayashi, Yujiro
Wang, Cong
Ishizuya, Yu
Kinouchi, Toshiro
Hayashi, Takuji
Matsuzaki, Kyosuke
Jingushi, Kentaro
Kato, Taigo
Kawashima, Atsunari
Ujike, Takeshi
Nagahara, Akira
Fujita, Kazutoshi
Imamura, Ryoichi
Nonomura, Norio
author_facet Yamamoto, Yoshiyuki
Uemura, Motohide
Nakano, Kosuke
Hayashi, Yujiro
Wang, Cong
Ishizuya, Yu
Kinouchi, Toshiro
Hayashi, Takuji
Matsuzaki, Kyosuke
Jingushi, Kentaro
Kato, Taigo
Kawashima, Atsunari
Ujike, Takeshi
Nagahara, Akira
Fujita, Kazutoshi
Imamura, Ryoichi
Nonomura, Norio
author_sort Yamamoto, Yoshiyuki
collection PubMed
description BACKGROUND: Reliable biomarkers for renal cell carcinoma (RCC) have yet to be found. Circulating cell-free DNA (cfDNA) is an emerging resource for the diagnosis and prognosis of various cancers. This study aims to identify novel blood biomarkers for RCC. MATERIALS AND METHODS: Plasma cfDNA was extracted from RCC patients (n = 92) and healthy controls (n = 41). Levels of cfDNA were determined using quantitative real-time PCR of ACTB as the target gene, and cfDNA fragment size was measured using a microfluidics-based platform. Diagnostic potential was assessed using receiver operating characteristic (ROC) and logistic regression analysis, and prognostic potential was evaluated using log-rank test. RESULTS: Median levels of cfDNA from RCC patients were significantly higher than those from healthy controls (3803 vs 2242 copies/ml, p < 0.001). Median fragment sizes of cfDNA in RCC patients were shorter than those in healthy controls (170 vs 171 bp, p = 0.052). To evaluate level of cfDNA as a diagnostic tool for RCC, ROC curve analysis revealed a sensitivity of 63.0% and a specificity of 78.1%. Multivariate analysis indicated that age, gender and the level of cfDNA were significantly associated with the presence of RCC (p < 0.001, p = 0.013, p < 0.001, respectively). Additionally, shorter cfDNA fragment size was negatively associated with progression-free survival (p = 0.006). CONCLUSIONS: Our study demonstrates the diagnostic and prognostic potential of plasma cfDNA as a biomarker for RCC.
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spelling pubmed-59455312018-05-13 Increased level and fragmentation of plasma circulating cell-free DNA are diagnostic and prognostic markers for renal cell carcinoma Yamamoto, Yoshiyuki Uemura, Motohide Nakano, Kosuke Hayashi, Yujiro Wang, Cong Ishizuya, Yu Kinouchi, Toshiro Hayashi, Takuji Matsuzaki, Kyosuke Jingushi, Kentaro Kato, Taigo Kawashima, Atsunari Ujike, Takeshi Nagahara, Akira Fujita, Kazutoshi Imamura, Ryoichi Nonomura, Norio Oncotarget Research Paper BACKGROUND: Reliable biomarkers for renal cell carcinoma (RCC) have yet to be found. Circulating cell-free DNA (cfDNA) is an emerging resource for the diagnosis and prognosis of various cancers. This study aims to identify novel blood biomarkers for RCC. MATERIALS AND METHODS: Plasma cfDNA was extracted from RCC patients (n = 92) and healthy controls (n = 41). Levels of cfDNA were determined using quantitative real-time PCR of ACTB as the target gene, and cfDNA fragment size was measured using a microfluidics-based platform. Diagnostic potential was assessed using receiver operating characteristic (ROC) and logistic regression analysis, and prognostic potential was evaluated using log-rank test. RESULTS: Median levels of cfDNA from RCC patients were significantly higher than those from healthy controls (3803 vs 2242 copies/ml, p < 0.001). Median fragment sizes of cfDNA in RCC patients were shorter than those in healthy controls (170 vs 171 bp, p = 0.052). To evaluate level of cfDNA as a diagnostic tool for RCC, ROC curve analysis revealed a sensitivity of 63.0% and a specificity of 78.1%. Multivariate analysis indicated that age, gender and the level of cfDNA were significantly associated with the presence of RCC (p < 0.001, p = 0.013, p < 0.001, respectively). Additionally, shorter cfDNA fragment size was negatively associated with progression-free survival (p = 0.006). CONCLUSIONS: Our study demonstrates the diagnostic and prognostic potential of plasma cfDNA as a biomarker for RCC. Impact Journals LLC 2018-04-17 /pmc/articles/PMC5945531/ /pubmed/29755665 http://dx.doi.org/10.18632/oncotarget.24943 Text en Copyright: © 2018 Yamamoto et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Yamamoto, Yoshiyuki
Uemura, Motohide
Nakano, Kosuke
Hayashi, Yujiro
Wang, Cong
Ishizuya, Yu
Kinouchi, Toshiro
Hayashi, Takuji
Matsuzaki, Kyosuke
Jingushi, Kentaro
Kato, Taigo
Kawashima, Atsunari
Ujike, Takeshi
Nagahara, Akira
Fujita, Kazutoshi
Imamura, Ryoichi
Nonomura, Norio
Increased level and fragmentation of plasma circulating cell-free DNA are diagnostic and prognostic markers for renal cell carcinoma
title Increased level and fragmentation of plasma circulating cell-free DNA are diagnostic and prognostic markers for renal cell carcinoma
title_full Increased level and fragmentation of plasma circulating cell-free DNA are diagnostic and prognostic markers for renal cell carcinoma
title_fullStr Increased level and fragmentation of plasma circulating cell-free DNA are diagnostic and prognostic markers for renal cell carcinoma
title_full_unstemmed Increased level and fragmentation of plasma circulating cell-free DNA are diagnostic and prognostic markers for renal cell carcinoma
title_short Increased level and fragmentation of plasma circulating cell-free DNA are diagnostic and prognostic markers for renal cell carcinoma
title_sort increased level and fragmentation of plasma circulating cell-free dna are diagnostic and prognostic markers for renal cell carcinoma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5945531/
https://www.ncbi.nlm.nih.gov/pubmed/29755665
http://dx.doi.org/10.18632/oncotarget.24943
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