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Increased level and fragmentation of plasma circulating cell-free DNA are diagnostic and prognostic markers for renal cell carcinoma
BACKGROUND: Reliable biomarkers for renal cell carcinoma (RCC) have yet to be found. Circulating cell-free DNA (cfDNA) is an emerging resource for the diagnosis and prognosis of various cancers. This study aims to identify novel blood biomarkers for RCC. MATERIALS AND METHODS: Plasma cfDNA was extra...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5945531/ https://www.ncbi.nlm.nih.gov/pubmed/29755665 http://dx.doi.org/10.18632/oncotarget.24943 |
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author | Yamamoto, Yoshiyuki Uemura, Motohide Nakano, Kosuke Hayashi, Yujiro Wang, Cong Ishizuya, Yu Kinouchi, Toshiro Hayashi, Takuji Matsuzaki, Kyosuke Jingushi, Kentaro Kato, Taigo Kawashima, Atsunari Ujike, Takeshi Nagahara, Akira Fujita, Kazutoshi Imamura, Ryoichi Nonomura, Norio |
author_facet | Yamamoto, Yoshiyuki Uemura, Motohide Nakano, Kosuke Hayashi, Yujiro Wang, Cong Ishizuya, Yu Kinouchi, Toshiro Hayashi, Takuji Matsuzaki, Kyosuke Jingushi, Kentaro Kato, Taigo Kawashima, Atsunari Ujike, Takeshi Nagahara, Akira Fujita, Kazutoshi Imamura, Ryoichi Nonomura, Norio |
author_sort | Yamamoto, Yoshiyuki |
collection | PubMed |
description | BACKGROUND: Reliable biomarkers for renal cell carcinoma (RCC) have yet to be found. Circulating cell-free DNA (cfDNA) is an emerging resource for the diagnosis and prognosis of various cancers. This study aims to identify novel blood biomarkers for RCC. MATERIALS AND METHODS: Plasma cfDNA was extracted from RCC patients (n = 92) and healthy controls (n = 41). Levels of cfDNA were determined using quantitative real-time PCR of ACTB as the target gene, and cfDNA fragment size was measured using a microfluidics-based platform. Diagnostic potential was assessed using receiver operating characteristic (ROC) and logistic regression analysis, and prognostic potential was evaluated using log-rank test. RESULTS: Median levels of cfDNA from RCC patients were significantly higher than those from healthy controls (3803 vs 2242 copies/ml, p < 0.001). Median fragment sizes of cfDNA in RCC patients were shorter than those in healthy controls (170 vs 171 bp, p = 0.052). To evaluate level of cfDNA as a diagnostic tool for RCC, ROC curve analysis revealed a sensitivity of 63.0% and a specificity of 78.1%. Multivariate analysis indicated that age, gender and the level of cfDNA were significantly associated with the presence of RCC (p < 0.001, p = 0.013, p < 0.001, respectively). Additionally, shorter cfDNA fragment size was negatively associated with progression-free survival (p = 0.006). CONCLUSIONS: Our study demonstrates the diagnostic and prognostic potential of plasma cfDNA as a biomarker for RCC. |
format | Online Article Text |
id | pubmed-5945531 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-59455312018-05-13 Increased level and fragmentation of plasma circulating cell-free DNA are diagnostic and prognostic markers for renal cell carcinoma Yamamoto, Yoshiyuki Uemura, Motohide Nakano, Kosuke Hayashi, Yujiro Wang, Cong Ishizuya, Yu Kinouchi, Toshiro Hayashi, Takuji Matsuzaki, Kyosuke Jingushi, Kentaro Kato, Taigo Kawashima, Atsunari Ujike, Takeshi Nagahara, Akira Fujita, Kazutoshi Imamura, Ryoichi Nonomura, Norio Oncotarget Research Paper BACKGROUND: Reliable biomarkers for renal cell carcinoma (RCC) have yet to be found. Circulating cell-free DNA (cfDNA) is an emerging resource for the diagnosis and prognosis of various cancers. This study aims to identify novel blood biomarkers for RCC. MATERIALS AND METHODS: Plasma cfDNA was extracted from RCC patients (n = 92) and healthy controls (n = 41). Levels of cfDNA were determined using quantitative real-time PCR of ACTB as the target gene, and cfDNA fragment size was measured using a microfluidics-based platform. Diagnostic potential was assessed using receiver operating characteristic (ROC) and logistic regression analysis, and prognostic potential was evaluated using log-rank test. RESULTS: Median levels of cfDNA from RCC patients were significantly higher than those from healthy controls (3803 vs 2242 copies/ml, p < 0.001). Median fragment sizes of cfDNA in RCC patients were shorter than those in healthy controls (170 vs 171 bp, p = 0.052). To evaluate level of cfDNA as a diagnostic tool for RCC, ROC curve analysis revealed a sensitivity of 63.0% and a specificity of 78.1%. Multivariate analysis indicated that age, gender and the level of cfDNA were significantly associated with the presence of RCC (p < 0.001, p = 0.013, p < 0.001, respectively). Additionally, shorter cfDNA fragment size was negatively associated with progression-free survival (p = 0.006). CONCLUSIONS: Our study demonstrates the diagnostic and prognostic potential of plasma cfDNA as a biomarker for RCC. Impact Journals LLC 2018-04-17 /pmc/articles/PMC5945531/ /pubmed/29755665 http://dx.doi.org/10.18632/oncotarget.24943 Text en Copyright: © 2018 Yamamoto et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Yamamoto, Yoshiyuki Uemura, Motohide Nakano, Kosuke Hayashi, Yujiro Wang, Cong Ishizuya, Yu Kinouchi, Toshiro Hayashi, Takuji Matsuzaki, Kyosuke Jingushi, Kentaro Kato, Taigo Kawashima, Atsunari Ujike, Takeshi Nagahara, Akira Fujita, Kazutoshi Imamura, Ryoichi Nonomura, Norio Increased level and fragmentation of plasma circulating cell-free DNA are diagnostic and prognostic markers for renal cell carcinoma |
title | Increased level and fragmentation of plasma circulating cell-free DNA are diagnostic and prognostic markers for renal cell carcinoma |
title_full | Increased level and fragmentation of plasma circulating cell-free DNA are diagnostic and prognostic markers for renal cell carcinoma |
title_fullStr | Increased level and fragmentation of plasma circulating cell-free DNA are diagnostic and prognostic markers for renal cell carcinoma |
title_full_unstemmed | Increased level and fragmentation of plasma circulating cell-free DNA are diagnostic and prognostic markers for renal cell carcinoma |
title_short | Increased level and fragmentation of plasma circulating cell-free DNA are diagnostic and prognostic markers for renal cell carcinoma |
title_sort | increased level and fragmentation of plasma circulating cell-free dna are diagnostic and prognostic markers for renal cell carcinoma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5945531/ https://www.ncbi.nlm.nih.gov/pubmed/29755665 http://dx.doi.org/10.18632/oncotarget.24943 |
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