Cargando…
Activated dendritic cells modulate proliferation and differentiation of human myoblasts
Idiopathic Inflammatory Myopathies (IIMs) are a heterogeneous group of autoimmune diseases affecting skeletal muscle tissue homeostasis. They are characterized by muscle weakness and inflammatory infiltration with tissue damage. Amongst the cells in the muscle inflammatory infiltration, dendritic ce...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5945640/ https://www.ncbi.nlm.nih.gov/pubmed/29748534 http://dx.doi.org/10.1038/s41419-018-0426-z |
_version_ | 1783322031116255232 |
---|---|
author | Ladislau, Leandro Portilho, Débora M. Courau, Tristan Solares-Pérez, Alhondra Negroni, Elisa Lainé, Jeanne Klatzmann, David Bonomo, Adriana Allenbach, Yves Benveniste, Olivier Riederer, Ingo Savino, Wilson Mouly, Vincent Butler-Browne, Gillian Benjamim, Claudia F. |
author_facet | Ladislau, Leandro Portilho, Débora M. Courau, Tristan Solares-Pérez, Alhondra Negroni, Elisa Lainé, Jeanne Klatzmann, David Bonomo, Adriana Allenbach, Yves Benveniste, Olivier Riederer, Ingo Savino, Wilson Mouly, Vincent Butler-Browne, Gillian Benjamim, Claudia F. |
author_sort | Ladislau, Leandro |
collection | PubMed |
description | Idiopathic Inflammatory Myopathies (IIMs) are a heterogeneous group of autoimmune diseases affecting skeletal muscle tissue homeostasis. They are characterized by muscle weakness and inflammatory infiltration with tissue damage. Amongst the cells in the muscle inflammatory infiltration, dendritic cells (DCs) are potent antigen-presenting and key components in autoimmunity exhibiting an increased activation in inflamed tissues. Since, the IIMs are characterized by the focal necrosis/regeneration and muscle atrophy, we hypothesized that DCs may play a role in these processes. Due to the absence of a reliable in vivo model for IIMs, we first performed co-culture experiments with immature DCs (iDC) or LPS-activated DCs (actDC) and proliferating myoblasts or differentiating myotubes. We demonstrated that both iDC or actDCs tightly interact with myoblasts and myotubes, increased myoblast proliferation and migration, but inhibited myotube differentiation. We also observed that actDCs increased HLA-ABC, HLA-DR, VLA-5, and VLA-6 expression and induced cytokine secretion on myoblasts. In an in vivo regeneration model, the co-injection of human myoblasts and DCs enhanced human myoblast migration, whereas the absolute number of human myofibres was unchanged. In conclusion, we suggest that in the early stages of myositis, DCs may play a crucial role in inducing muscle-damage through cell–cell contact and inflammatory cytokine secretion, leading to muscle regeneration impairment. |
format | Online Article Text |
id | pubmed-5945640 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59456402018-05-11 Activated dendritic cells modulate proliferation and differentiation of human myoblasts Ladislau, Leandro Portilho, Débora M. Courau, Tristan Solares-Pérez, Alhondra Negroni, Elisa Lainé, Jeanne Klatzmann, David Bonomo, Adriana Allenbach, Yves Benveniste, Olivier Riederer, Ingo Savino, Wilson Mouly, Vincent Butler-Browne, Gillian Benjamim, Claudia F. Cell Death Dis Article Idiopathic Inflammatory Myopathies (IIMs) are a heterogeneous group of autoimmune diseases affecting skeletal muscle tissue homeostasis. They are characterized by muscle weakness and inflammatory infiltration with tissue damage. Amongst the cells in the muscle inflammatory infiltration, dendritic cells (DCs) are potent antigen-presenting and key components in autoimmunity exhibiting an increased activation in inflamed tissues. Since, the IIMs are characterized by the focal necrosis/regeneration and muscle atrophy, we hypothesized that DCs may play a role in these processes. Due to the absence of a reliable in vivo model for IIMs, we first performed co-culture experiments with immature DCs (iDC) or LPS-activated DCs (actDC) and proliferating myoblasts or differentiating myotubes. We demonstrated that both iDC or actDCs tightly interact with myoblasts and myotubes, increased myoblast proliferation and migration, but inhibited myotube differentiation. We also observed that actDCs increased HLA-ABC, HLA-DR, VLA-5, and VLA-6 expression and induced cytokine secretion on myoblasts. In an in vivo regeneration model, the co-injection of human myoblasts and DCs enhanced human myoblast migration, whereas the absolute number of human myofibres was unchanged. In conclusion, we suggest that in the early stages of myositis, DCs may play a crucial role in inducing muscle-damage through cell–cell contact and inflammatory cytokine secretion, leading to muscle regeneration impairment. Nature Publishing Group UK 2018-05-10 /pmc/articles/PMC5945640/ /pubmed/29748534 http://dx.doi.org/10.1038/s41419-018-0426-z Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Ladislau, Leandro Portilho, Débora M. Courau, Tristan Solares-Pérez, Alhondra Negroni, Elisa Lainé, Jeanne Klatzmann, David Bonomo, Adriana Allenbach, Yves Benveniste, Olivier Riederer, Ingo Savino, Wilson Mouly, Vincent Butler-Browne, Gillian Benjamim, Claudia F. Activated dendritic cells modulate proliferation and differentiation of human myoblasts |
title | Activated dendritic cells modulate proliferation and differentiation of human myoblasts |
title_full | Activated dendritic cells modulate proliferation and differentiation of human myoblasts |
title_fullStr | Activated dendritic cells modulate proliferation and differentiation of human myoblasts |
title_full_unstemmed | Activated dendritic cells modulate proliferation and differentiation of human myoblasts |
title_short | Activated dendritic cells modulate proliferation and differentiation of human myoblasts |
title_sort | activated dendritic cells modulate proliferation and differentiation of human myoblasts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5945640/ https://www.ncbi.nlm.nih.gov/pubmed/29748534 http://dx.doi.org/10.1038/s41419-018-0426-z |
work_keys_str_mv | AT ladislauleandro activateddendriticcellsmodulateproliferationanddifferentiationofhumanmyoblasts AT portilhodeboram activateddendriticcellsmodulateproliferationanddifferentiationofhumanmyoblasts AT courautristan activateddendriticcellsmodulateproliferationanddifferentiationofhumanmyoblasts AT solaresperezalhondra activateddendriticcellsmodulateproliferationanddifferentiationofhumanmyoblasts AT negronielisa activateddendriticcellsmodulateproliferationanddifferentiationofhumanmyoblasts AT lainejeanne activateddendriticcellsmodulateproliferationanddifferentiationofhumanmyoblasts AT klatzmanndavid activateddendriticcellsmodulateproliferationanddifferentiationofhumanmyoblasts AT bonomoadriana activateddendriticcellsmodulateproliferationanddifferentiationofhumanmyoblasts AT allenbachyves activateddendriticcellsmodulateproliferationanddifferentiationofhumanmyoblasts AT benvenisteolivier activateddendriticcellsmodulateproliferationanddifferentiationofhumanmyoblasts AT riedereringo activateddendriticcellsmodulateproliferationanddifferentiationofhumanmyoblasts AT savinowilson activateddendriticcellsmodulateproliferationanddifferentiationofhumanmyoblasts AT moulyvincent activateddendriticcellsmodulateproliferationanddifferentiationofhumanmyoblasts AT butlerbrownegillian activateddendriticcellsmodulateproliferationanddifferentiationofhumanmyoblasts AT benjamimclaudiaf activateddendriticcellsmodulateproliferationanddifferentiationofhumanmyoblasts |