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Salivary alpha-amylase activity and cortisol in horses with acute abdominal disease: a pilot study
BACKGROUND: The aim of this study was to evaluate salivary alpha-amylase (sAA), considered a non-invasive biomarker for sympathetic nervous system (SNS) activity, and salivary cortisol as possible pain-induced stress biomarker, in horses with acute abdominal disease. Therefore, a prospective observa...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5946548/ https://www.ncbi.nlm.nih.gov/pubmed/29747642 http://dx.doi.org/10.1186/s12917-018-1482-4 |
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author | Contreras-Aguilar, María Dolores Escribano, Damián Martín-Cuervo, María Tecles, Fernando Cerón, Jose Joaquín |
author_facet | Contreras-Aguilar, María Dolores Escribano, Damián Martín-Cuervo, María Tecles, Fernando Cerón, Jose Joaquín |
author_sort | Contreras-Aguilar, María Dolores |
collection | PubMed |
description | BACKGROUND: The aim of this study was to evaluate salivary alpha-amylase (sAA), considered a non-invasive biomarker for sympathetic nervous system (SNS) activity, and salivary cortisol as possible pain-induced stress biomarker, in horses with acute abdominal disease. Therefore, a prospective observational study was performed in which both biomarkers were analyzed in a group of horses with acute abdomen syndrome, and compared with a group of healthy control horses by an unpaired Student’s t-test. In addition, the possible relationship between both biomarkers, the score in Equine Acute Abdominal Pain scales version 1 (EAAPS-1 scale), Heart Rate (HR) and Respiratory Rate (RR), plasma lactate, the systemic inflammatory response syndrome (SIRS) score and serum amyloid A (SAA) concentration was assessed by a Spearman correlation test. RESULTS: A total of 30 horses were included in the study, 19 with acute abdominal disease diagnosed as large colon displacements, simple impactions of the pelvic flexure, spasmodic colics and enteritis and 11 healthy ones. sAA activity (24.5 median-fold, P < 0.0001) and salivary cortisol (1.7 median-fold, P < 0.01) were significantly higher in horses with acute abdomen than in healthy horses. sAA activity was significantly correlated with EAAPS-1 scale (r = 0.78, 95% confidence interval [CI] 0.38–0.89, P < 0.001) and SIRS score (r = 0.49, 95% CI 0.03–0.78, P < 0.05). Neither sAA nor salivary cortisol correlated with HR, RR, plasma lactate and SAA. CONCLUSIONS: Although this study should be considered as preliminary one, alpha-amylase measurements in saliva could be a biomarker of pain-induced stress in horses with acute abdominal disease. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12917-018-1482-4) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5946548 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-59465482018-05-17 Salivary alpha-amylase activity and cortisol in horses with acute abdominal disease: a pilot study Contreras-Aguilar, María Dolores Escribano, Damián Martín-Cuervo, María Tecles, Fernando Cerón, Jose Joaquín BMC Vet Res Research Article BACKGROUND: The aim of this study was to evaluate salivary alpha-amylase (sAA), considered a non-invasive biomarker for sympathetic nervous system (SNS) activity, and salivary cortisol as possible pain-induced stress biomarker, in horses with acute abdominal disease. Therefore, a prospective observational study was performed in which both biomarkers were analyzed in a group of horses with acute abdomen syndrome, and compared with a group of healthy control horses by an unpaired Student’s t-test. In addition, the possible relationship between both biomarkers, the score in Equine Acute Abdominal Pain scales version 1 (EAAPS-1 scale), Heart Rate (HR) and Respiratory Rate (RR), plasma lactate, the systemic inflammatory response syndrome (SIRS) score and serum amyloid A (SAA) concentration was assessed by a Spearman correlation test. RESULTS: A total of 30 horses were included in the study, 19 with acute abdominal disease diagnosed as large colon displacements, simple impactions of the pelvic flexure, spasmodic colics and enteritis and 11 healthy ones. sAA activity (24.5 median-fold, P < 0.0001) and salivary cortisol (1.7 median-fold, P < 0.01) were significantly higher in horses with acute abdomen than in healthy horses. sAA activity was significantly correlated with EAAPS-1 scale (r = 0.78, 95% confidence interval [CI] 0.38–0.89, P < 0.001) and SIRS score (r = 0.49, 95% CI 0.03–0.78, P < 0.05). Neither sAA nor salivary cortisol correlated with HR, RR, plasma lactate and SAA. CONCLUSIONS: Although this study should be considered as preliminary one, alpha-amylase measurements in saliva could be a biomarker of pain-induced stress in horses with acute abdominal disease. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12917-018-1482-4) contains supplementary material, which is available to authorized users. BioMed Central 2018-05-10 /pmc/articles/PMC5946548/ /pubmed/29747642 http://dx.doi.org/10.1186/s12917-018-1482-4 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Contreras-Aguilar, María Dolores Escribano, Damián Martín-Cuervo, María Tecles, Fernando Cerón, Jose Joaquín Salivary alpha-amylase activity and cortisol in horses with acute abdominal disease: a pilot study |
title | Salivary alpha-amylase activity and cortisol in horses with acute abdominal disease: a pilot study |
title_full | Salivary alpha-amylase activity and cortisol in horses with acute abdominal disease: a pilot study |
title_fullStr | Salivary alpha-amylase activity and cortisol in horses with acute abdominal disease: a pilot study |
title_full_unstemmed | Salivary alpha-amylase activity and cortisol in horses with acute abdominal disease: a pilot study |
title_short | Salivary alpha-amylase activity and cortisol in horses with acute abdominal disease: a pilot study |
title_sort | salivary alpha-amylase activity and cortisol in horses with acute abdominal disease: a pilot study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5946548/ https://www.ncbi.nlm.nih.gov/pubmed/29747642 http://dx.doi.org/10.1186/s12917-018-1482-4 |
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