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The relationships between faecal egg counts and gut microbial composition in UK Thoroughbreds infected by cyathostomins

A growing body of evidence, particularly in humans and rodents, supports the existence of a complex network of interactions occurring between gastrointestinal (GI) helminth parasites and the gut commensal bacteria, with substantial effects on both host immunity and metabolic potential. However, litt...

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Autores principales: Peachey, L.E., Molena, R.A., Jenkins, T.P., Di Cesare, A., Traversa, D., Hodgkinson, J.E., Cantacessi, C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5946844/
https://www.ncbi.nlm.nih.gov/pubmed/29432771
http://dx.doi.org/10.1016/j.ijpara.2017.11.003
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author Peachey, L.E.
Molena, R.A.
Jenkins, T.P.
Di Cesare, A.
Traversa, D.
Hodgkinson, J.E.
Cantacessi, C.
author_facet Peachey, L.E.
Molena, R.A.
Jenkins, T.P.
Di Cesare, A.
Traversa, D.
Hodgkinson, J.E.
Cantacessi, C.
author_sort Peachey, L.E.
collection PubMed
description A growing body of evidence, particularly in humans and rodents, supports the existence of a complex network of interactions occurring between gastrointestinal (GI) helminth parasites and the gut commensal bacteria, with substantial effects on both host immunity and metabolic potential. However, little is known of the fundamental biology of such interactions in other animal species; nonetheless, given the considerable economic losses associated with GI parasites, particularly in livestock and equines, as well as the global threat of emerging anthelmintic resistance, further explorations of the complexities of host-helminth-microbiota interactions in these species are needed. This study characterises the composition of the equine gut commensal flora associated with the presence, in faecal samples, of low (Clow) and high (Chigh) numbers of eggs of an important group of GI parasites (i.e. the cyathostomins), prior to and following anthelmintic treatment. High-throughput sequencing of bacterial 16S rRNA amplicons and associated bioinformatics and statistical analyses of sequence data revealed strong clustering according to faecal egg counts (P = 0.003). A trend towards increased populations of Methanomicrobia (class) and Dehalobacterium (genus) was observed in Clow in comparison with Chigh. Anthelmintic treatment in Chigh was associated with a significant reduction of the bacterial Phylum TM7 14 days post-ivermectin administration, as well as a transient expansion of Adlercreutzia spp. at 2 days post-treatment. This study provides a first known insight into the discovery of the intimate mechanisms governing host-parasite-microbiota interactions in equines, and sets a basis for the development of novel, biology-based intervention strategies against equine GI helminths based on the manipulation of the commensal gut flora.
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spelling pubmed-59468442018-05-14 The relationships between faecal egg counts and gut microbial composition in UK Thoroughbreds infected by cyathostomins Peachey, L.E. Molena, R.A. Jenkins, T.P. Di Cesare, A. Traversa, D. Hodgkinson, J.E. Cantacessi, C. Int J Parasitol Article A growing body of evidence, particularly in humans and rodents, supports the existence of a complex network of interactions occurring between gastrointestinal (GI) helminth parasites and the gut commensal bacteria, with substantial effects on both host immunity and metabolic potential. However, little is known of the fundamental biology of such interactions in other animal species; nonetheless, given the considerable economic losses associated with GI parasites, particularly in livestock and equines, as well as the global threat of emerging anthelmintic resistance, further explorations of the complexities of host-helminth-microbiota interactions in these species are needed. This study characterises the composition of the equine gut commensal flora associated with the presence, in faecal samples, of low (Clow) and high (Chigh) numbers of eggs of an important group of GI parasites (i.e. the cyathostomins), prior to and following anthelmintic treatment. High-throughput sequencing of bacterial 16S rRNA amplicons and associated bioinformatics and statistical analyses of sequence data revealed strong clustering according to faecal egg counts (P = 0.003). A trend towards increased populations of Methanomicrobia (class) and Dehalobacterium (genus) was observed in Clow in comparison with Chigh. Anthelmintic treatment in Chigh was associated with a significant reduction of the bacterial Phylum TM7 14 days post-ivermectin administration, as well as a transient expansion of Adlercreutzia spp. at 2 days post-treatment. This study provides a first known insight into the discovery of the intimate mechanisms governing host-parasite-microbiota interactions in equines, and sets a basis for the development of novel, biology-based intervention strategies against equine GI helminths based on the manipulation of the commensal gut flora. Elsevier Science 2018-05 /pmc/articles/PMC5946844/ /pubmed/29432771 http://dx.doi.org/10.1016/j.ijpara.2017.11.003 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Peachey, L.E.
Molena, R.A.
Jenkins, T.P.
Di Cesare, A.
Traversa, D.
Hodgkinson, J.E.
Cantacessi, C.
The relationships between faecal egg counts and gut microbial composition in UK Thoroughbreds infected by cyathostomins
title The relationships between faecal egg counts and gut microbial composition in UK Thoroughbreds infected by cyathostomins
title_full The relationships between faecal egg counts and gut microbial composition in UK Thoroughbreds infected by cyathostomins
title_fullStr The relationships between faecal egg counts and gut microbial composition in UK Thoroughbreds infected by cyathostomins
title_full_unstemmed The relationships between faecal egg counts and gut microbial composition in UK Thoroughbreds infected by cyathostomins
title_short The relationships between faecal egg counts and gut microbial composition in UK Thoroughbreds infected by cyathostomins
title_sort relationships between faecal egg counts and gut microbial composition in uk thoroughbreds infected by cyathostomins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5946844/
https://www.ncbi.nlm.nih.gov/pubmed/29432771
http://dx.doi.org/10.1016/j.ijpara.2017.11.003
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