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Development of microsatellite markers for a Chinese endemic plant, Torreya yunnanensis (Taxaceae)

PREMISE OF THE STUDY: Microsatellite markers were developed in Torreya yunnanensis (Taxaceae) to investigate the genetic diversity, phylogeography, and population structure of the species. METHODS AND RESULTS: Sixteen primer sets were identified using Illumina 2 × 100‐bp paired‐end sequencing and bi...

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Autores principales: Wang, Yu Peng, Cheng, Hai Xiang, Li, Jian Hui, Zhou, Shou Biao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5947606/
https://www.ncbi.nlm.nih.gov/pubmed/30131887
http://dx.doi.org/10.1002/aps3.1145
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author Wang, Yu Peng
Cheng, Hai Xiang
Li, Jian Hui
Zhou, Shou Biao
author_facet Wang, Yu Peng
Cheng, Hai Xiang
Li, Jian Hui
Zhou, Shou Biao
author_sort Wang, Yu Peng
collection PubMed
description PREMISE OF THE STUDY: Microsatellite markers were developed in Torreya yunnanensis (Taxaceae) to investigate the genetic diversity, phylogeography, and population structure of the species. METHODS AND RESULTS: Sixteen primer sets were identified using Illumina 2 × 100‐bp paired‐end sequencing and bioinformatic screening. Most primers also amplified in T. fargesii, T. grandis, T. jackii, and T. nucifera. CONCLUSIONS: These results indicate the utility of these microsatellite markers in T. yunnanensis for future studies of molecular ecology as well as their applicability across the genus.
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spelling pubmed-59476062018-08-21 Development of microsatellite markers for a Chinese endemic plant, Torreya yunnanensis (Taxaceae) Wang, Yu Peng Cheng, Hai Xiang Li, Jian Hui Zhou, Shou Biao Appl Plant Sci Primer Notes PREMISE OF THE STUDY: Microsatellite markers were developed in Torreya yunnanensis (Taxaceae) to investigate the genetic diversity, phylogeography, and population structure of the species. METHODS AND RESULTS: Sixteen primer sets were identified using Illumina 2 × 100‐bp paired‐end sequencing and bioinformatic screening. Most primers also amplified in T. fargesii, T. grandis, T. jackii, and T. nucifera. CONCLUSIONS: These results indicate the utility of these microsatellite markers in T. yunnanensis for future studies of molecular ecology as well as their applicability across the genus. John Wiley and Sons Inc. 2018-05-10 /pmc/articles/PMC5947606/ /pubmed/30131887 http://dx.doi.org/10.1002/aps3.1145 Text en © 2018 Wang et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Primer Notes
Wang, Yu Peng
Cheng, Hai Xiang
Li, Jian Hui
Zhou, Shou Biao
Development of microsatellite markers for a Chinese endemic plant, Torreya yunnanensis (Taxaceae)
title Development of microsatellite markers for a Chinese endemic plant, Torreya yunnanensis (Taxaceae)
title_full Development of microsatellite markers for a Chinese endemic plant, Torreya yunnanensis (Taxaceae)
title_fullStr Development of microsatellite markers for a Chinese endemic plant, Torreya yunnanensis (Taxaceae)
title_full_unstemmed Development of microsatellite markers for a Chinese endemic plant, Torreya yunnanensis (Taxaceae)
title_short Development of microsatellite markers for a Chinese endemic plant, Torreya yunnanensis (Taxaceae)
title_sort development of microsatellite markers for a chinese endemic plant, torreya yunnanensis (taxaceae)
topic Primer Notes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5947606/
https://www.ncbi.nlm.nih.gov/pubmed/30131887
http://dx.doi.org/10.1002/aps3.1145
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