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Random mutagenesis screen shows that Phytophthora capsici CRN83_152‐mediated cell death is not required for its virulence function(s)

With the increasing availability of plant pathogen genomes, secreted proteins that aid infection (effectors) have emerged as key factors that help to govern plant–microbe interactions. The conserved CRN (CRinkling and Necrosis) effector family was first described in oomycetes by their capacity to in...

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Autores principales: Amaro, Tiago M. M. M., Thilliez, Gaëtan J. A., Mcleod, Rory A., Huitema, Edgar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5947615/
https://www.ncbi.nlm.nih.gov/pubmed/28779542
http://dx.doi.org/10.1111/mpp.12590
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author Amaro, Tiago M. M. M.
Thilliez, Gaëtan J. A.
Mcleod, Rory A.
Huitema, Edgar
author_facet Amaro, Tiago M. M. M.
Thilliez, Gaëtan J. A.
Mcleod, Rory A.
Huitema, Edgar
author_sort Amaro, Tiago M. M. M.
collection PubMed
description With the increasing availability of plant pathogen genomes, secreted proteins that aid infection (effectors) have emerged as key factors that help to govern plant–microbe interactions. The conserved CRN (CRinkling and Necrosis) effector family was first described in oomycetes by their capacity to induce host cell death. Despite recent advances towards the elucidation of CRN virulence functions, the relevance of CRN‐induced cell death remains unclear. In planta over‐expression of PcCRN83_152, a CRN effector from Phytophthora capsici, causes host cell death and boosts P. capsici virulence. We used these features to ask whether PcCRN83_152‐induced cell death is linked to its virulence function. By randomly mutating this effector, we generated PcCRN83_152 variants with no cell death (NCD) phenotypes, which were subsequently tested for activity towards enhanced virulence. We showed that a subset of PcCRN83_152 NCD variants retained their ability to boost P. capsici virulence. Moreover, NCD variants were shown to have a suppressive effect on PcCRN83_152‐mediated cell death. Our work shows that PcCRN83_152‐induced cell death and virulence function can be separated. Moreover, if these findings hold true for other cell death‐inducing CRN effectors, this work, in turn, will provide a framework for studies aimed at unveiling the virulence functions of these effectors.
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spelling pubmed-59476152018-05-17 Random mutagenesis screen shows that Phytophthora capsici CRN83_152‐mediated cell death is not required for its virulence function(s) Amaro, Tiago M. M. M. Thilliez, Gaëtan J. A. Mcleod, Rory A. Huitema, Edgar Mol Plant Pathol Original Articles With the increasing availability of plant pathogen genomes, secreted proteins that aid infection (effectors) have emerged as key factors that help to govern plant–microbe interactions. The conserved CRN (CRinkling and Necrosis) effector family was first described in oomycetes by their capacity to induce host cell death. Despite recent advances towards the elucidation of CRN virulence functions, the relevance of CRN‐induced cell death remains unclear. In planta over‐expression of PcCRN83_152, a CRN effector from Phytophthora capsici, causes host cell death and boosts P. capsici virulence. We used these features to ask whether PcCRN83_152‐induced cell death is linked to its virulence function. By randomly mutating this effector, we generated PcCRN83_152 variants with no cell death (NCD) phenotypes, which were subsequently tested for activity towards enhanced virulence. We showed that a subset of PcCRN83_152 NCD variants retained their ability to boost P. capsici virulence. Moreover, NCD variants were shown to have a suppressive effect on PcCRN83_152‐mediated cell death. Our work shows that PcCRN83_152‐induced cell death and virulence function can be separated. Moreover, if these findings hold true for other cell death‐inducing CRN effectors, this work, in turn, will provide a framework for studies aimed at unveiling the virulence functions of these effectors. John Wiley and Sons Inc. 2017-10-24 /pmc/articles/PMC5947615/ /pubmed/28779542 http://dx.doi.org/10.1111/mpp.12590 Text en © 2017 THE AUTHORS. MOLECULAR PLANT PATHOLOGY PUBLISHED BY BRITISH SOCIETY FOR PLANT PATHOLOGY AND JOHN WILEY & SONS LTD This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Amaro, Tiago M. M. M.
Thilliez, Gaëtan J. A.
Mcleod, Rory A.
Huitema, Edgar
Random mutagenesis screen shows that Phytophthora capsici CRN83_152‐mediated cell death is not required for its virulence function(s)
title Random mutagenesis screen shows that Phytophthora capsici CRN83_152‐mediated cell death is not required for its virulence function(s)
title_full Random mutagenesis screen shows that Phytophthora capsici CRN83_152‐mediated cell death is not required for its virulence function(s)
title_fullStr Random mutagenesis screen shows that Phytophthora capsici CRN83_152‐mediated cell death is not required for its virulence function(s)
title_full_unstemmed Random mutagenesis screen shows that Phytophthora capsici CRN83_152‐mediated cell death is not required for its virulence function(s)
title_short Random mutagenesis screen shows that Phytophthora capsici CRN83_152‐mediated cell death is not required for its virulence function(s)
title_sort random mutagenesis screen shows that phytophthora capsici crn83_152‐mediated cell death is not required for its virulence function(s)
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5947615/
https://www.ncbi.nlm.nih.gov/pubmed/28779542
http://dx.doi.org/10.1111/mpp.12590
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