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NP30 stimulates Th17 differentiation through DC in Schistosomiasis Japonicum

The murine monoclonal anti‐idiotypic antibody, NP30, is a potential vaccine candidate against Schistosoma japonicum. Previous studies have revealed that NP30 has an immunoregulatory effect, but the underlying mechanism for this effect remains unknown. This study shows that NP30 induces dendritic cel...

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Detalles Bibliográficos
Autores principales: Xu, L., Xue, B., Zhou, L., Qiu, Z., Zhang, X., Xu, N., Tang, Q., Zhu, J., Guan, X., Feng, Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5947655/
https://www.ncbi.nlm.nih.gov/pubmed/29577333
http://dx.doi.org/10.1111/pim.12528
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author Xu, L.
Xue, B.
Zhou, L.
Qiu, Z.
Zhang, X.
Xu, N.
Tang, Q.
Zhu, J.
Guan, X.
Feng, Z.
author_facet Xu, L.
Xue, B.
Zhou, L.
Qiu, Z.
Zhang, X.
Xu, N.
Tang, Q.
Zhu, J.
Guan, X.
Feng, Z.
author_sort Xu, L.
collection PubMed
description The murine monoclonal anti‐idiotypic antibody, NP30, is a potential vaccine candidate against Schistosoma japonicum. Previous studies have revealed that NP30 has an immunoregulatory effect, but the underlying mechanism for this effect remains unknown. This study shows that NP30 induces dendritic cell (DC) maturation and increases the production of pro‐inflammatory cytokines. The expression of CD86 and MHC II was upregulated in DCs following stimulation with NP30 in vitro. Moreover, NP30 induced Th17 polarization by increasing the production of IL‐6 and TGF‐β. In vivo, Th17 differentiation was induced by the production of key pro‐inflammatory cytokines, including IL‐6and TGF‐β, from DCs of NP30‐immunized mice. These results indicate that NP30 promotes Th17 polarization through DC activation, preventing serious schistosomiasis.
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spelling pubmed-59476552018-05-17 NP30 stimulates Th17 differentiation through DC in Schistosomiasis Japonicum Xu, L. Xue, B. Zhou, L. Qiu, Z. Zhang, X. Xu, N. Tang, Q. Zhu, J. Guan, X. Feng, Z. Parasite Immunol Original Articles The murine monoclonal anti‐idiotypic antibody, NP30, is a potential vaccine candidate against Schistosoma japonicum. Previous studies have revealed that NP30 has an immunoregulatory effect, but the underlying mechanism for this effect remains unknown. This study shows that NP30 induces dendritic cell (DC) maturation and increases the production of pro‐inflammatory cytokines. The expression of CD86 and MHC II was upregulated in DCs following stimulation with NP30 in vitro. Moreover, NP30 induced Th17 polarization by increasing the production of IL‐6 and TGF‐β. In vivo, Th17 differentiation was induced by the production of key pro‐inflammatory cytokines, including IL‐6and TGF‐β, from DCs of NP30‐immunized mice. These results indicate that NP30 promotes Th17 polarization through DC activation, preventing serious schistosomiasis. John Wiley and Sons Inc. 2018-04-25 2018-05 /pmc/articles/PMC5947655/ /pubmed/29577333 http://dx.doi.org/10.1111/pim.12528 Text en © 2018 The Authors. Parasite Immunology Published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Xu, L.
Xue, B.
Zhou, L.
Qiu, Z.
Zhang, X.
Xu, N.
Tang, Q.
Zhu, J.
Guan, X.
Feng, Z.
NP30 stimulates Th17 differentiation through DC in Schistosomiasis Japonicum
title NP30 stimulates Th17 differentiation through DC in Schistosomiasis Japonicum
title_full NP30 stimulates Th17 differentiation through DC in Schistosomiasis Japonicum
title_fullStr NP30 stimulates Th17 differentiation through DC in Schistosomiasis Japonicum
title_full_unstemmed NP30 stimulates Th17 differentiation through DC in Schistosomiasis Japonicum
title_short NP30 stimulates Th17 differentiation through DC in Schistosomiasis Japonicum
title_sort np30 stimulates th17 differentiation through dc in schistosomiasis japonicum
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5947655/
https://www.ncbi.nlm.nih.gov/pubmed/29577333
http://dx.doi.org/10.1111/pim.12528
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