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The impact of HLA-G, LILRB1 and LILRB2 gene polymorphisms on susceptibility to and severity of endometriosis

Endometriosis is a disease in which endometriotic tissue occurs outside the uterus. Its pathogenesis is still unknown. The most widespread hypothesis claims that ectopic endometrium appears as a result of retrograde menstruation and its insufficient elimination by immunocytes. Some reports have show...

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Autores principales: Bylińska, Aleksandra, Wilczyńska, Karolina, Malejczyk, Jacek, Milewski, Łukasz, Wagner, Marta, Jasek, Monika, Niepiekło-Miniewska, Wanda, Wiśniewski, Andrzej, Płoski, Rafał, Barcz, Ewa, Roszkowski, Piotr, Kamiński, Paweł, Malinowski, Andrzej, Wilczyński, Jacek R., Radwan, Paweł, Radwan, Michał, Kuśnierczyk, Piotr, Nowak, Izabela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5948266/
https://www.ncbi.nlm.nih.gov/pubmed/29234882
http://dx.doi.org/10.1007/s00438-017-1404-3
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author Bylińska, Aleksandra
Wilczyńska, Karolina
Malejczyk, Jacek
Milewski, Łukasz
Wagner, Marta
Jasek, Monika
Niepiekło-Miniewska, Wanda
Wiśniewski, Andrzej
Płoski, Rafał
Barcz, Ewa
Roszkowski, Piotr
Kamiński, Paweł
Malinowski, Andrzej
Wilczyński, Jacek R.
Radwan, Paweł
Radwan, Michał
Kuśnierczyk, Piotr
Nowak, Izabela
author_facet Bylińska, Aleksandra
Wilczyńska, Karolina
Malejczyk, Jacek
Milewski, Łukasz
Wagner, Marta
Jasek, Monika
Niepiekło-Miniewska, Wanda
Wiśniewski, Andrzej
Płoski, Rafał
Barcz, Ewa
Roszkowski, Piotr
Kamiński, Paweł
Malinowski, Andrzej
Wilczyński, Jacek R.
Radwan, Paweł
Radwan, Michał
Kuśnierczyk, Piotr
Nowak, Izabela
author_sort Bylińska, Aleksandra
collection PubMed
description Endometriosis is a disease in which endometriotic tissue occurs outside the uterus. Its pathogenesis is still unknown. The most widespread hypothesis claims that ectopic endometrium appears as a result of retrograde menstruation and its insufficient elimination by immunocytes. Some reports have shown expression of non-classical HLA-G molecules on ectopic endometrium. HLA-G is recognized by KIR2DL4, LILRB1 and LILRB2 receptors on natural killer (NK) and other cells. These receptors are polymorphic, which may affect their activity. In this study we investigated whether HLA-G, KIR2DL4, LILRB1 and LILRB2 polymorphisms may influence susceptibility to endometriosis and disease progression. We used polymerase chain reaction (PCR), PCR-restriction fragment length polymorphism (PCR-RFLP) and allelic discrimination methods with TaqMan SNP Genotyping Assays for typing of 276 patients with endometriosis and 314 healthy fertile women. The HLA-G rs1632947:GG genotype was associated with protection against the disease and its severe stages; HLA-G rs1233334:CT protected against progression; LILRB1 rs41308748:AA and LILRB2 rs383369:AG predisposed to the disease and its progression. No effect of KIR2DL4 polymorphism was observed. These results support the role of polymorphisms of HLA-G and its receptors LILRB1 and LILRB2 in susceptibility to endometriosis and its progression. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00438-017-1404-3) contains supplementary material, which is available to authorized users.
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spelling pubmed-59482662018-05-17 The impact of HLA-G, LILRB1 and LILRB2 gene polymorphisms on susceptibility to and severity of endometriosis Bylińska, Aleksandra Wilczyńska, Karolina Malejczyk, Jacek Milewski, Łukasz Wagner, Marta Jasek, Monika Niepiekło-Miniewska, Wanda Wiśniewski, Andrzej Płoski, Rafał Barcz, Ewa Roszkowski, Piotr Kamiński, Paweł Malinowski, Andrzej Wilczyński, Jacek R. Radwan, Paweł Radwan, Michał Kuśnierczyk, Piotr Nowak, Izabela Mol Genet Genomics Original Article Endometriosis is a disease in which endometriotic tissue occurs outside the uterus. Its pathogenesis is still unknown. The most widespread hypothesis claims that ectopic endometrium appears as a result of retrograde menstruation and its insufficient elimination by immunocytes. Some reports have shown expression of non-classical HLA-G molecules on ectopic endometrium. HLA-G is recognized by KIR2DL4, LILRB1 and LILRB2 receptors on natural killer (NK) and other cells. These receptors are polymorphic, which may affect their activity. In this study we investigated whether HLA-G, KIR2DL4, LILRB1 and LILRB2 polymorphisms may influence susceptibility to endometriosis and disease progression. We used polymerase chain reaction (PCR), PCR-restriction fragment length polymorphism (PCR-RFLP) and allelic discrimination methods with TaqMan SNP Genotyping Assays for typing of 276 patients with endometriosis and 314 healthy fertile women. The HLA-G rs1632947:GG genotype was associated with protection against the disease and its severe stages; HLA-G rs1233334:CT protected against progression; LILRB1 rs41308748:AA and LILRB2 rs383369:AG predisposed to the disease and its progression. No effect of KIR2DL4 polymorphism was observed. These results support the role of polymorphisms of HLA-G and its receptors LILRB1 and LILRB2 in susceptibility to endometriosis and its progression. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00438-017-1404-3) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2017-12-12 2018 /pmc/articles/PMC5948266/ /pubmed/29234882 http://dx.doi.org/10.1007/s00438-017-1404-3 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Bylińska, Aleksandra
Wilczyńska, Karolina
Malejczyk, Jacek
Milewski, Łukasz
Wagner, Marta
Jasek, Monika
Niepiekło-Miniewska, Wanda
Wiśniewski, Andrzej
Płoski, Rafał
Barcz, Ewa
Roszkowski, Piotr
Kamiński, Paweł
Malinowski, Andrzej
Wilczyński, Jacek R.
Radwan, Paweł
Radwan, Michał
Kuśnierczyk, Piotr
Nowak, Izabela
The impact of HLA-G, LILRB1 and LILRB2 gene polymorphisms on susceptibility to and severity of endometriosis
title The impact of HLA-G, LILRB1 and LILRB2 gene polymorphisms on susceptibility to and severity of endometriosis
title_full The impact of HLA-G, LILRB1 and LILRB2 gene polymorphisms on susceptibility to and severity of endometriosis
title_fullStr The impact of HLA-G, LILRB1 and LILRB2 gene polymorphisms on susceptibility to and severity of endometriosis
title_full_unstemmed The impact of HLA-G, LILRB1 and LILRB2 gene polymorphisms on susceptibility to and severity of endometriosis
title_short The impact of HLA-G, LILRB1 and LILRB2 gene polymorphisms on susceptibility to and severity of endometriosis
title_sort impact of hla-g, lilrb1 and lilrb2 gene polymorphisms on susceptibility to and severity of endometriosis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5948266/
https://www.ncbi.nlm.nih.gov/pubmed/29234882
http://dx.doi.org/10.1007/s00438-017-1404-3
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