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Integrated analysis of transcriptomic and metabolomic data reveals critical metabolic pathways involved in rotenoid biosynthesis in the medicinal plant Mirabilis himalaica

Mirabilis himalaica (Edgew.) Heimerl is among the most important genuine medicinal plants in Tibet. However, the biosynthesis mechanisms of the active compounds in this species are unclear, severely limiting its application. To clarify the molecular biosynthesis mechanism of the key representative a...

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Autores principales: Gu, Li, Zhang, Zhong-yi, Quan, Hong, Li, Ming-jie, Zhao, Fang-yu, Xu, Yuan-jiang, Liu, Jiang, Sai, Man, Zheng, Wei-lie, Lan, Xiao-zhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5948277/
https://www.ncbi.nlm.nih.gov/pubmed/29285563
http://dx.doi.org/10.1007/s00438-017-1409-y
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author Gu, Li
Zhang, Zhong-yi
Quan, Hong
Li, Ming-jie
Zhao, Fang-yu
Xu, Yuan-jiang
Liu, Jiang
Sai, Man
Zheng, Wei-lie
Lan, Xiao-zhong
author_facet Gu, Li
Zhang, Zhong-yi
Quan, Hong
Li, Ming-jie
Zhao, Fang-yu
Xu, Yuan-jiang
Liu, Jiang
Sai, Man
Zheng, Wei-lie
Lan, Xiao-zhong
author_sort Gu, Li
collection PubMed
description Mirabilis himalaica (Edgew.) Heimerl is among the most important genuine medicinal plants in Tibet. However, the biosynthesis mechanisms of the active compounds in this species are unclear, severely limiting its application. To clarify the molecular biosynthesis mechanism of the key representative active compounds, specifically rotenoid, which is of special medicinal value for M. himalaica, RNA sequencing and TOF-MS technologies were used to construct transcriptomic and metabolomic libraries from the roots, stems, and leaves of M. himalaica plants collected from their natural habitat. As a result, each of the transcriptomic libraries from the different tissues was sequenced, generating more than 10 Gb of clean data ultimately assembled into 147,142 unigenes. In the three tissues, metabolomic analysis identified 522 candidate compounds, of which 170 metabolites involved in 114 metabolic pathways were mapped to the KEGG. Of these genes, 61 encoding enzymes were identified to function at key steps of the pathways related to rotenoid biosynthesis, where 14 intermediate metabolites were also located. An integrated analysis of metabolic and transcriptomic data revealed that most of the intermediate metabolites and enzymes related to rotenoid biosynthesis were synthesized in the roots, stems and leaves of M. himalaica, which suggested that the use of non-medicinal tissues to extract compounds was feasible. In addition, the CHS and CHI genes were found to play important roles in rotenoid biosynthesis, especially, since CHS might be an important rate-limiting enzyme. This study provides a hypothetical basis for the screening of new active metabolites and the metabolic engineering of rotenoid in M. himalaica. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00438-017-1409-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-59482772018-05-17 Integrated analysis of transcriptomic and metabolomic data reveals critical metabolic pathways involved in rotenoid biosynthesis in the medicinal plant Mirabilis himalaica Gu, Li Zhang, Zhong-yi Quan, Hong Li, Ming-jie Zhao, Fang-yu Xu, Yuan-jiang Liu, Jiang Sai, Man Zheng, Wei-lie Lan, Xiao-zhong Mol Genet Genomics Original Article Mirabilis himalaica (Edgew.) Heimerl is among the most important genuine medicinal plants in Tibet. However, the biosynthesis mechanisms of the active compounds in this species are unclear, severely limiting its application. To clarify the molecular biosynthesis mechanism of the key representative active compounds, specifically rotenoid, which is of special medicinal value for M. himalaica, RNA sequencing and TOF-MS technologies were used to construct transcriptomic and metabolomic libraries from the roots, stems, and leaves of M. himalaica plants collected from their natural habitat. As a result, each of the transcriptomic libraries from the different tissues was sequenced, generating more than 10 Gb of clean data ultimately assembled into 147,142 unigenes. In the three tissues, metabolomic analysis identified 522 candidate compounds, of which 170 metabolites involved in 114 metabolic pathways were mapped to the KEGG. Of these genes, 61 encoding enzymes were identified to function at key steps of the pathways related to rotenoid biosynthesis, where 14 intermediate metabolites were also located. An integrated analysis of metabolic and transcriptomic data revealed that most of the intermediate metabolites and enzymes related to rotenoid biosynthesis were synthesized in the roots, stems and leaves of M. himalaica, which suggested that the use of non-medicinal tissues to extract compounds was feasible. In addition, the CHS and CHI genes were found to play important roles in rotenoid biosynthesis, especially, since CHS might be an important rate-limiting enzyme. This study provides a hypothetical basis for the screening of new active metabolites and the metabolic engineering of rotenoid in M. himalaica. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00438-017-1409-y) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2017-12-28 2018 /pmc/articles/PMC5948277/ /pubmed/29285563 http://dx.doi.org/10.1007/s00438-017-1409-y Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Gu, Li
Zhang, Zhong-yi
Quan, Hong
Li, Ming-jie
Zhao, Fang-yu
Xu, Yuan-jiang
Liu, Jiang
Sai, Man
Zheng, Wei-lie
Lan, Xiao-zhong
Integrated analysis of transcriptomic and metabolomic data reveals critical metabolic pathways involved in rotenoid biosynthesis in the medicinal plant Mirabilis himalaica
title Integrated analysis of transcriptomic and metabolomic data reveals critical metabolic pathways involved in rotenoid biosynthesis in the medicinal plant Mirabilis himalaica
title_full Integrated analysis of transcriptomic and metabolomic data reveals critical metabolic pathways involved in rotenoid biosynthesis in the medicinal plant Mirabilis himalaica
title_fullStr Integrated analysis of transcriptomic and metabolomic data reveals critical metabolic pathways involved in rotenoid biosynthesis in the medicinal plant Mirabilis himalaica
title_full_unstemmed Integrated analysis of transcriptomic and metabolomic data reveals critical metabolic pathways involved in rotenoid biosynthesis in the medicinal plant Mirabilis himalaica
title_short Integrated analysis of transcriptomic and metabolomic data reveals critical metabolic pathways involved in rotenoid biosynthesis in the medicinal plant Mirabilis himalaica
title_sort integrated analysis of transcriptomic and metabolomic data reveals critical metabolic pathways involved in rotenoid biosynthesis in the medicinal plant mirabilis himalaica
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5948277/
https://www.ncbi.nlm.nih.gov/pubmed/29285563
http://dx.doi.org/10.1007/s00438-017-1409-y
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