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From Protein Features to Sensing Surfaces

Proteins play a major role in biosensors in which they provide catalytic activity and specificity in molecular recognition. However, the immobilization process is far from straightforward as it often affects the protein functionality. Extensive interaction of the protein with the surface or signific...

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Detalles Bibliográficos
Autor principal: Faccio, Greta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5948494/
https://www.ncbi.nlm.nih.gov/pubmed/29662030
http://dx.doi.org/10.3390/s18041204
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author Faccio, Greta
author_facet Faccio, Greta
author_sort Faccio, Greta
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description Proteins play a major role in biosensors in which they provide catalytic activity and specificity in molecular recognition. However, the immobilization process is far from straightforward as it often affects the protein functionality. Extensive interaction of the protein with the surface or significant surface crowding can lead to changes in the mobility and conformation of the protein structure. This review will provide insights as to how an analysis of the physico-chemical features of the protein surface before the immobilization process can help to identify the optimal immobilization approach. Such an analysis can help to preserve the functionality of the protein when on a biosensor surface.
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spelling pubmed-59484942018-05-17 From Protein Features to Sensing Surfaces Faccio, Greta Sensors (Basel) Review Proteins play a major role in biosensors in which they provide catalytic activity and specificity in molecular recognition. However, the immobilization process is far from straightforward as it often affects the protein functionality. Extensive interaction of the protein with the surface or significant surface crowding can lead to changes in the mobility and conformation of the protein structure. This review will provide insights as to how an analysis of the physico-chemical features of the protein surface before the immobilization process can help to identify the optimal immobilization approach. Such an analysis can help to preserve the functionality of the protein when on a biosensor surface. MDPI 2018-04-15 /pmc/articles/PMC5948494/ /pubmed/29662030 http://dx.doi.org/10.3390/s18041204 Text en © 2018 by the author. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Faccio, Greta
From Protein Features to Sensing Surfaces
title From Protein Features to Sensing Surfaces
title_full From Protein Features to Sensing Surfaces
title_fullStr From Protein Features to Sensing Surfaces
title_full_unstemmed From Protein Features to Sensing Surfaces
title_short From Protein Features to Sensing Surfaces
title_sort from protein features to sensing surfaces
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5948494/
https://www.ncbi.nlm.nih.gov/pubmed/29662030
http://dx.doi.org/10.3390/s18041204
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