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Label-Free QCM Immunosensor for the Detection of Ochratoxin A
Ochratoxin A (OTA) is a potent mycotoxin that poses a risk in food and feed moieties and subject to worldwide regulation. Laboratory-based analytical methods are traditionally employed for reliable OTA quantification, but these methods cannot provide rapid and on-site analysis, where biosensors fill...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5948555/ https://www.ncbi.nlm.nih.gov/pubmed/29641432 http://dx.doi.org/10.3390/s18041161 |
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author | Pirinçci, Şerife Şeyda Ertekin, Özlem Laguna, Duygu Ercan Özen, Fehime Şeyma Öztürk, Zafer Ziya Öztürk, Selma |
author_facet | Pirinçci, Şerife Şeyda Ertekin, Özlem Laguna, Duygu Ercan Özen, Fehime Şeyma Öztürk, Zafer Ziya Öztürk, Selma |
author_sort | Pirinçci, Şerife Şeyda |
collection | PubMed |
description | Ochratoxin A (OTA) is a potent mycotoxin that poses a risk in food and feed moieties and subject to worldwide regulation. Laboratory-based analytical methods are traditionally employed for reliable OTA quantification, but these methods cannot provide rapid and on-site analysis, where biosensors fill this gap. In this study a label-free quartz crystal microbalance (QCM)-based immunosensor for the detection of OTA, which is one of the most important small molecule contaminants, was developed by direct immobilization of OTA to amine-bearing sensor surfaces using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) chemistry. The protein-free sensor surface enabled regeneration of sensor surface with 50 mM NaOH and 1% SDS up to 13 times without loss of performance, which would disrupt a protein-containing sensor surface. We developed a QCM immunosensor using the developed sensor surface with a 17.2–200 ng/mL detection range which can be used for on-site detection of feedstuffs. |
format | Online Article Text |
id | pubmed-5948555 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-59485552018-05-17 Label-Free QCM Immunosensor for the Detection of Ochratoxin A Pirinçci, Şerife Şeyda Ertekin, Özlem Laguna, Duygu Ercan Özen, Fehime Şeyma Öztürk, Zafer Ziya Öztürk, Selma Sensors (Basel) Article Ochratoxin A (OTA) is a potent mycotoxin that poses a risk in food and feed moieties and subject to worldwide regulation. Laboratory-based analytical methods are traditionally employed for reliable OTA quantification, but these methods cannot provide rapid and on-site analysis, where biosensors fill this gap. In this study a label-free quartz crystal microbalance (QCM)-based immunosensor for the detection of OTA, which is one of the most important small molecule contaminants, was developed by direct immobilization of OTA to amine-bearing sensor surfaces using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) chemistry. The protein-free sensor surface enabled regeneration of sensor surface with 50 mM NaOH and 1% SDS up to 13 times without loss of performance, which would disrupt a protein-containing sensor surface. We developed a QCM immunosensor using the developed sensor surface with a 17.2–200 ng/mL detection range which can be used for on-site detection of feedstuffs. MDPI 2018-04-11 /pmc/articles/PMC5948555/ /pubmed/29641432 http://dx.doi.org/10.3390/s18041161 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pirinçci, Şerife Şeyda Ertekin, Özlem Laguna, Duygu Ercan Özen, Fehime Şeyma Öztürk, Zafer Ziya Öztürk, Selma Label-Free QCM Immunosensor for the Detection of Ochratoxin A |
title | Label-Free QCM Immunosensor for the Detection of Ochratoxin A |
title_full | Label-Free QCM Immunosensor for the Detection of Ochratoxin A |
title_fullStr | Label-Free QCM Immunosensor for the Detection of Ochratoxin A |
title_full_unstemmed | Label-Free QCM Immunosensor for the Detection of Ochratoxin A |
title_short | Label-Free QCM Immunosensor for the Detection of Ochratoxin A |
title_sort | label-free qcm immunosensor for the detection of ochratoxin a |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5948555/ https://www.ncbi.nlm.nih.gov/pubmed/29641432 http://dx.doi.org/10.3390/s18041161 |
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