Cargando…

Label-Free QCM Immunosensor for the Detection of Ochratoxin A

Ochratoxin A (OTA) is a potent mycotoxin that poses a risk in food and feed moieties and subject to worldwide regulation. Laboratory-based analytical methods are traditionally employed for reliable OTA quantification, but these methods cannot provide rapid and on-site analysis, where biosensors fill...

Descripción completa

Detalles Bibliográficos
Autores principales: Pirinçci, Şerife Şeyda, Ertekin, Özlem, Laguna, Duygu Ercan, Özen, Fehime Şeyma, Öztürk, Zafer Ziya, Öztürk, Selma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5948555/
https://www.ncbi.nlm.nih.gov/pubmed/29641432
http://dx.doi.org/10.3390/s18041161
_version_ 1783322576184934400
author Pirinçci, Şerife Şeyda
Ertekin, Özlem
Laguna, Duygu Ercan
Özen, Fehime Şeyma
Öztürk, Zafer Ziya
Öztürk, Selma
author_facet Pirinçci, Şerife Şeyda
Ertekin, Özlem
Laguna, Duygu Ercan
Özen, Fehime Şeyma
Öztürk, Zafer Ziya
Öztürk, Selma
author_sort Pirinçci, Şerife Şeyda
collection PubMed
description Ochratoxin A (OTA) is a potent mycotoxin that poses a risk in food and feed moieties and subject to worldwide regulation. Laboratory-based analytical methods are traditionally employed for reliable OTA quantification, but these methods cannot provide rapid and on-site analysis, where biosensors fill this gap. In this study a label-free quartz crystal microbalance (QCM)-based immunosensor for the detection of OTA, which is one of the most important small molecule contaminants, was developed by direct immobilization of OTA to amine-bearing sensor surfaces using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) chemistry. The protein-free sensor surface enabled regeneration of sensor surface with 50 mM NaOH and 1% SDS up to 13 times without loss of performance, which would disrupt a protein-containing sensor surface. We developed a QCM immunosensor using the developed sensor surface with a 17.2–200 ng/mL detection range which can be used for on-site detection of feedstuffs.
format Online
Article
Text
id pubmed-5948555
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-59485552018-05-17 Label-Free QCM Immunosensor for the Detection of Ochratoxin A Pirinçci, Şerife Şeyda Ertekin, Özlem Laguna, Duygu Ercan Özen, Fehime Şeyma Öztürk, Zafer Ziya Öztürk, Selma Sensors (Basel) Article Ochratoxin A (OTA) is a potent mycotoxin that poses a risk in food and feed moieties and subject to worldwide regulation. Laboratory-based analytical methods are traditionally employed for reliable OTA quantification, but these methods cannot provide rapid and on-site analysis, where biosensors fill this gap. In this study a label-free quartz crystal microbalance (QCM)-based immunosensor for the detection of OTA, which is one of the most important small molecule contaminants, was developed by direct immobilization of OTA to amine-bearing sensor surfaces using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) chemistry. The protein-free sensor surface enabled regeneration of sensor surface with 50 mM NaOH and 1% SDS up to 13 times without loss of performance, which would disrupt a protein-containing sensor surface. We developed a QCM immunosensor using the developed sensor surface with a 17.2–200 ng/mL detection range which can be used for on-site detection of feedstuffs. MDPI 2018-04-11 /pmc/articles/PMC5948555/ /pubmed/29641432 http://dx.doi.org/10.3390/s18041161 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pirinçci, Şerife Şeyda
Ertekin, Özlem
Laguna, Duygu Ercan
Özen, Fehime Şeyma
Öztürk, Zafer Ziya
Öztürk, Selma
Label-Free QCM Immunosensor for the Detection of Ochratoxin A
title Label-Free QCM Immunosensor for the Detection of Ochratoxin A
title_full Label-Free QCM Immunosensor for the Detection of Ochratoxin A
title_fullStr Label-Free QCM Immunosensor for the Detection of Ochratoxin A
title_full_unstemmed Label-Free QCM Immunosensor for the Detection of Ochratoxin A
title_short Label-Free QCM Immunosensor for the Detection of Ochratoxin A
title_sort label-free qcm immunosensor for the detection of ochratoxin a
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5948555/
https://www.ncbi.nlm.nih.gov/pubmed/29641432
http://dx.doi.org/10.3390/s18041161
work_keys_str_mv AT pirincciserifeseyda labelfreeqcmimmunosensorforthedetectionofochratoxina
AT ertekinozlem labelfreeqcmimmunosensorforthedetectionofochratoxina
AT lagunaduyguercan labelfreeqcmimmunosensorforthedetectionofochratoxina
AT ozenfehimeseyma labelfreeqcmimmunosensorforthedetectionofochratoxina
AT ozturkzaferziya labelfreeqcmimmunosensorforthedetectionofochratoxina
AT ozturkselma labelfreeqcmimmunosensorforthedetectionofochratoxina