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An In-Vitro Optical Sensor Designed to Estimate Glycated Hemoglobin Levels

The purpose of this research was to design an optical sensor for evaluating glycated hemoglobin (HbA1c) percentages in hemoglobin. The A1c sensors available in the market use invasive methods, while our device offers the possibility of non-invasive monitoring of HbA1c levels in diabetic patients. A...

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Detalles Bibliográficos
Autores principales: Mandal, Sanghamitra, Manasreh, M. O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5948830/
https://www.ncbi.nlm.nih.gov/pubmed/29617292
http://dx.doi.org/10.3390/s18041084
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author Mandal, Sanghamitra
Manasreh, M. O.
author_facet Mandal, Sanghamitra
Manasreh, M. O.
author_sort Mandal, Sanghamitra
collection PubMed
description The purpose of this research was to design an optical sensor for evaluating glycated hemoglobin (HbA1c) percentages in hemoglobin. The A1c sensors available in the market use invasive methods, while our device offers the possibility of non-invasive monitoring of HbA1c levels in diabetic patients. A prototype is assembled using two light emitting diodes with peak emission wavelengths of 535 nm and 593 nm, a photodiode, and a microcontroller. The proposed sensor measures the transmitted intensity in the form of an output voltage. We devise an approach to estimate the percentage of HbA1c in hemoglobin for a given solution. This estimation is based on the relative change in absorbance due to change in path length and molar absorption coefficients of hemoglobin and HbA1c, at the two wavelengths. We calculate the molar absorption coefficient of HbA1c at 535 nm and 593 nm wavelengths using the sensor, which is performed by a multiple variable regression analysis algorithm fed through the microcontroller. Specifically, the sensor output voltage with respect to the sample concentration is fitted to an exponentially decaying equation model. We used a commercial chemical assay called Control FD Glycohemoglobin A1c with known percentage HbA1c levels to verify our device measurements.
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spelling pubmed-59488302018-05-17 An In-Vitro Optical Sensor Designed to Estimate Glycated Hemoglobin Levels Mandal, Sanghamitra Manasreh, M. O. Sensors (Basel) Article The purpose of this research was to design an optical sensor for evaluating glycated hemoglobin (HbA1c) percentages in hemoglobin. The A1c sensors available in the market use invasive methods, while our device offers the possibility of non-invasive monitoring of HbA1c levels in diabetic patients. A prototype is assembled using two light emitting diodes with peak emission wavelengths of 535 nm and 593 nm, a photodiode, and a microcontroller. The proposed sensor measures the transmitted intensity in the form of an output voltage. We devise an approach to estimate the percentage of HbA1c in hemoglobin for a given solution. This estimation is based on the relative change in absorbance due to change in path length and molar absorption coefficients of hemoglobin and HbA1c, at the two wavelengths. We calculate the molar absorption coefficient of HbA1c at 535 nm and 593 nm wavelengths using the sensor, which is performed by a multiple variable regression analysis algorithm fed through the microcontroller. Specifically, the sensor output voltage with respect to the sample concentration is fitted to an exponentially decaying equation model. We used a commercial chemical assay called Control FD Glycohemoglobin A1c with known percentage HbA1c levels to verify our device measurements. MDPI 2018-04-04 /pmc/articles/PMC5948830/ /pubmed/29617292 http://dx.doi.org/10.3390/s18041084 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mandal, Sanghamitra
Manasreh, M. O.
An In-Vitro Optical Sensor Designed to Estimate Glycated Hemoglobin Levels
title An In-Vitro Optical Sensor Designed to Estimate Glycated Hemoglobin Levels
title_full An In-Vitro Optical Sensor Designed to Estimate Glycated Hemoglobin Levels
title_fullStr An In-Vitro Optical Sensor Designed to Estimate Glycated Hemoglobin Levels
title_full_unstemmed An In-Vitro Optical Sensor Designed to Estimate Glycated Hemoglobin Levels
title_short An In-Vitro Optical Sensor Designed to Estimate Glycated Hemoglobin Levels
title_sort in-vitro optical sensor designed to estimate glycated hemoglobin levels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5948830/
https://www.ncbi.nlm.nih.gov/pubmed/29617292
http://dx.doi.org/10.3390/s18041084
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