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The rice XA21 ectodomain fused to the Arabidopsis EFR cytoplasmic domain confers resistance to Xanthomonas oryzae pv. oryzae

Rice (Oryza sativa) plants expressing the XA21 cell-surface receptor kinase are resistant to Xanthomonas oryzae pv. oryzae (Xoo) infection. We previously demonstrated that expressing a chimeric protein containing the ELONGATION FACTOR Tu RECEPTOR (EFR) ectodomain and the XA21 endodomain (EFR:XA21) i...

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Autores principales: Thomas, Nicholas C., Oksenberg, Nir, Liu, Furong, Caddell, Daniel, Nalyvayko, Alina, Nguyen, Yen, Schwessinger, Benjamin, Ronald, Pamela C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5949059/
https://www.ncbi.nlm.nih.gov/pubmed/29761034
http://dx.doi.org/10.7717/peerj.4456
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author Thomas, Nicholas C.
Oksenberg, Nir
Liu, Furong
Caddell, Daniel
Nalyvayko, Alina
Nguyen, Yen
Schwessinger, Benjamin
Ronald, Pamela C.
author_facet Thomas, Nicholas C.
Oksenberg, Nir
Liu, Furong
Caddell, Daniel
Nalyvayko, Alina
Nguyen, Yen
Schwessinger, Benjamin
Ronald, Pamela C.
author_sort Thomas, Nicholas C.
collection PubMed
description Rice (Oryza sativa) plants expressing the XA21 cell-surface receptor kinase are resistant to Xanthomonas oryzae pv. oryzae (Xoo) infection. We previously demonstrated that expressing a chimeric protein containing the ELONGATION FACTOR Tu RECEPTOR (EFR) ectodomain and the XA21 endodomain (EFR:XA21) in rice does not confer robust resistance to Xoo. To test if the XA21 ectodomain is required for Xoo resistance, we produced transgenic rice lines expressing a chimeric protein consisting of the XA21 ectodomain and EFR endodomain (XA21:EFR) and inoculated these lines with Xoo. We also tested if the XA21:EFR rice plants respond to a synthetic sulfated 21 amino acid derivative (RaxX21-sY) of the activator of XA21-mediated immunity, RaxX. We found that five independently transformed XA21:EFR rice lines displayed resistance to Xoo as measured by lesion length analysis, and showed that five lines share characteristic markers of the XA21 defense response (generation of reactive oxygen species and defense response gene expression) after treatment with RaxX21-sY. Our results indicate that expression of the XA21:EFR chimeric receptor in rice confers resistance to Xoo. These results suggest that the endodomain of the EFR and XA21 immune receptors are interchangeable and the XA21 ectodomain is the key determinant conferring robust resistance to Xoo.
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spelling pubmed-59490592018-05-14 The rice XA21 ectodomain fused to the Arabidopsis EFR cytoplasmic domain confers resistance to Xanthomonas oryzae pv. oryzae Thomas, Nicholas C. Oksenberg, Nir Liu, Furong Caddell, Daniel Nalyvayko, Alina Nguyen, Yen Schwessinger, Benjamin Ronald, Pamela C. PeerJ Agricultural Science Rice (Oryza sativa) plants expressing the XA21 cell-surface receptor kinase are resistant to Xanthomonas oryzae pv. oryzae (Xoo) infection. We previously demonstrated that expressing a chimeric protein containing the ELONGATION FACTOR Tu RECEPTOR (EFR) ectodomain and the XA21 endodomain (EFR:XA21) in rice does not confer robust resistance to Xoo. To test if the XA21 ectodomain is required for Xoo resistance, we produced transgenic rice lines expressing a chimeric protein consisting of the XA21 ectodomain and EFR endodomain (XA21:EFR) and inoculated these lines with Xoo. We also tested if the XA21:EFR rice plants respond to a synthetic sulfated 21 amino acid derivative (RaxX21-sY) of the activator of XA21-mediated immunity, RaxX. We found that five independently transformed XA21:EFR rice lines displayed resistance to Xoo as measured by lesion length analysis, and showed that five lines share characteristic markers of the XA21 defense response (generation of reactive oxygen species and defense response gene expression) after treatment with RaxX21-sY. Our results indicate that expression of the XA21:EFR chimeric receptor in rice confers resistance to Xoo. These results suggest that the endodomain of the EFR and XA21 immune receptors are interchangeable and the XA21 ectodomain is the key determinant conferring robust resistance to Xoo. PeerJ Inc. 2018-05-09 /pmc/articles/PMC5949059/ /pubmed/29761034 http://dx.doi.org/10.7717/peerj.4456 Text en © 2018 Thomas et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Thomas, Nicholas C.
Oksenberg, Nir
Liu, Furong
Caddell, Daniel
Nalyvayko, Alina
Nguyen, Yen
Schwessinger, Benjamin
Ronald, Pamela C.
The rice XA21 ectodomain fused to the Arabidopsis EFR cytoplasmic domain confers resistance to Xanthomonas oryzae pv. oryzae
title The rice XA21 ectodomain fused to the Arabidopsis EFR cytoplasmic domain confers resistance to Xanthomonas oryzae pv. oryzae
title_full The rice XA21 ectodomain fused to the Arabidopsis EFR cytoplasmic domain confers resistance to Xanthomonas oryzae pv. oryzae
title_fullStr The rice XA21 ectodomain fused to the Arabidopsis EFR cytoplasmic domain confers resistance to Xanthomonas oryzae pv. oryzae
title_full_unstemmed The rice XA21 ectodomain fused to the Arabidopsis EFR cytoplasmic domain confers resistance to Xanthomonas oryzae pv. oryzae
title_short The rice XA21 ectodomain fused to the Arabidopsis EFR cytoplasmic domain confers resistance to Xanthomonas oryzae pv. oryzae
title_sort rice xa21 ectodomain fused to the arabidopsis efr cytoplasmic domain confers resistance to xanthomonas oryzae pv. oryzae
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5949059/
https://www.ncbi.nlm.nih.gov/pubmed/29761034
http://dx.doi.org/10.7717/peerj.4456
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